Ebru E Yörüker1, Emre Özgür1, Metin Keskin2, Nejat Dalay1, Stefan Holdenrieder3, Ugur Gezer4. 1. Department of Basic Oncology, Oncology Institute, Istanbul University, Istanbul, Turkey. 2. Department of General Surgery, Istanbul Medical Faculty, Istanbul University, Istanbul, Turkey. 3. Institute of Clinical Chemistry and Pharmacology, University of Bonn, Bonn, Germany. 4. Department of Basic Oncology, Oncology Institute, Istanbul University, Istanbul, Turkey ugurd@istanbul.edu.tr.
Abstract
UNLABELLED: Background /Aim: Studies evaluating the integrity of cell-fee DNA (cfDNA) in colorectal cancer (CRC) have led to inconsistent results. Herein, we analyzed the utility of two different DNA integrity indexes (ACTB(384)/ACTB(106) and ALU(247)/ALU(115)) to assess cfDNA fragmentation in Turkish CRC patients. The correlation of circulating nucleosomes (cNUC) with the fragment sizes was also evaluated. MATERIALS AND METHODS: Seventy two CRC patients and 42 CRC-free control individuals were enrolled in the study. cfDNA was analyzed by quantitative polymerase chain reaction (qPCR). RESULTS: While with ALU(247)/ALU(115)there was a small difference between the groups, an approximately 3-fold (median values=0.12 and 0.34) lower integrity was found in the patients using the ACTB(384)/ACTB(106) ratio (p=0.06). The correlation between cNUC and qPCR threshold cycles was much higher for shorter fragments. CONCLUSION: Lower DNA integrity and the predominance of mononuclesomes in serum reveal high fragmentation of cfDNA in CRC patients. Copyright
UNLABELLED: Background /Aim: Studies evaluating the integrity of cell-fee DNA (cfDNA) in colorectal cancer (CRC) have led to inconsistent results. Herein, we analyzed the utility of two different DNA integrity indexes (ACTB(384)/ACTB(106) and ALU(247)/ALU(115)) to assess cfDNA fragmentation in Turkish CRC patients. The correlation of circulating nucleosomes (cNUC) with the fragment sizes was also evaluated. MATERIALS AND METHODS: Seventy two CRC patients and 42 CRC-free control individuals were enrolled in the study. cfDNA was analyzed by quantitative polymerase chain reaction (qPCR). RESULTS: While with ALU(247)/ALU(115)there was a small difference between the groups, an approximately 3-fold (median values=0.12 and 0.34) lower integrity was found in the patients using the ACTB(384)/ACTB(106) ratio (p=0.06). The correlation between cNUC and qPCR threshold cycles was much higher for shorter fragments. CONCLUSION: Lower DNA integrity and the predominance of mononuclesomes in serum reveal high fragmentation of cfDNA in CRC patients. Copyright
Authors: Michail Galanopoulos; Nikolaos Tsoukalas; Ioannis S Papanikolaou; Maria Tolia; Maria Gazouli; Gerassimos J Mantzaris Journal: World J Gastrointest Oncol Date: 2017-04-15