Fabrício Souza Silva1,2, Pedro Modesto Nascimento Menezes1, Pedro Guilherme Souza de Sá1, André Luís de Santana Oliveira3, Eric Alencar Araújo Souza1, Jackson Roberto Guedes da Silva Almeida1,4, Julianeli Tolentino de Lima1,4, Ana Paula Trovatti Uetanabaro5, Tânia Regina dos Santos Silva6, Edna Dória Peralta3, Angélica Maria Lucchese2,3. 1. a Núcleo de Estudos e Pesquisas em Plantas Medicinais, Colegiado de Ciências Farmacêuticas, Universidade Federal do Vale do São Francisco , Petrolina , Pernambuco , Brazil . 2. b Programa de Pós-Graduação em Biotecnologia, Departamento de Ciências Biológicas, Universidade Estadual de Feira de Santana , Feira de Santana , Bahia , Brazil . 3. c Laboratório de Química de Produtos Naturais e Sintéticos Bioativos, Departamento de Ciências Exatas , Universidade Estadual de Feira de Santana, Feira de Santana , Bahia , Brazil . 4. d Programa de Pós-Graduação em Recursos Naturais do Semi-Árido, Universidade Federal do Vale do São Francisco, Petrolina , Pernambuco , Brazil . 5. e Laboratório de Microbiologia da Agroindústria , Universidade Estadual de Santa Cruz , Ilhéus, Bahia , Brazil , and. 6. f Departamento de Ciências Biológicas , Universidade Estadual de Feira de Santana , Feira de Santana , Bahia , Brazil.
Abstract
CONTEXT: Lippia thymoides Mart. & Schauer (Verbenaceae) is used in folk medicine to treat wounds, fever, bronchitis, rheumatism, headaches, and weakness. OBJECTIVE: This study determinates the chemical composition of essential oils from L. thymoides, obtained at during each of the four seasons and correlates with pharmacological properties. MATERIALS AND METHODS: Essential oils were obtained by hydrodistillation and analyzed by gas chromatography coupled to mass spectroscopy (GC-MS). Antioxidant activity was determined by DPPH free radical scavenging and β-carotene bleaching methods. The antimicrobial assays were performed by minimum inhibitory concentration (MIC) and minimum microbicidal concentration (MMC) methods. Isolated rat aorta and uterus, and guinea-pig trachea were utilized to evaluate relaxant potential in pre-contracted smooth muscle. RESULTS AND DISCUSSION: Essential oils from leaves of L. thymoides had the sesquiterpene β-caryophyllene (17.22-26.27%) as the major constituent followed by borneol (4.45-7.36%), camphor (3.22-8.61%), camphene (2.64-5.66%), and germacrene D (4.72-6.18%). In vitro assays showed that these essential oils do not have antioxidant activity, have antimicrobial selectivity to Gram-positive bacteria Staphylococcus aureus (MIC = 0.004 mg/mL and MMC = 0.26-10.19 mg/mL) and Micrococcus luteus (MIC = 0.03 mg/mL and MMC = 8.43 mg/mL), relax isolated rat aorta (EC50 = 305-544 μg/mL, with endothelium; and EC50 = 150-283 μg/mL, without endothelium), and uterus (EC50 = 74-257 μg/mL), and minor potency, isolated guinea-pig trachea. CONCLUSIONS: Lippia thymoides is a source of natural products of pharmaceutical interest, being necessary additional studies to determine the substances involved in the biological activities.
CONTEXT: Lippia thymoides Mart. & Schauer (Verbenaceae) is used in folk medicine to treat wounds, fever, bronchitis, rheumatism, headaches, and weakness. OBJECTIVE: This study determinates the chemical composition of essential oils from L. thymoides, obtained at during each of the four seasons and correlates with pharmacological properties. MATERIALS AND METHODS:Essential oils were obtained by hydrodistillation and analyzed by gas chromatography coupled to mass spectroscopy (GC-MS). Antioxidant activity was determined by DPPH free radical scavenging and β-carotene bleaching methods. The antimicrobial assays were performed by minimum inhibitory concentration (MIC) and minimum microbicidal concentration (MMC) methods. Isolated rat aorta and uterus, and guinea-pig trachea were utilized to evaluate relaxant potential in pre-contracted smooth muscle. RESULTS AND DISCUSSION: Essential oils from leaves of L. thymoides had the sesquiterpene β-caryophyllene (17.22-26.27%) as the major constituent followed by borneol (4.45-7.36%), camphor (3.22-8.61%), camphene (2.64-5.66%), and germacrene D (4.72-6.18%). In vitro assays showed that these essential oils do not have antioxidant activity, have antimicrobial selectivity to Gram-positive bacteria Staphylococcus aureus (MIC = 0.004 mg/mL and MMC = 0.26-10.19 mg/mL) and Micrococcus luteus (MIC = 0.03 mg/mL and MMC = 8.43 mg/mL), relax isolated rat aorta (EC50 = 305-544 μg/mL, with endothelium; and EC50 = 150-283 μg/mL, without endothelium), and uterus (EC50 = 74-257 μg/mL), and minor potency, isolated guinea-pig trachea. CONCLUSIONS: Lippia thymoides is a source of natural products of pharmaceutical interest, being necessary additional studies to determine the substances involved in the biological activities.
Authors: Lidiane Diniz do Nascimento; Sebastião Gomes Silva; Márcia Moraes Cascaes; Kauê Santana da Costa; Pablo Luis Baia Figueiredo; Cristiane Maria Leal Costa; Eloisa Helena de Aguiar Andrade; Lênio José Guerreiro de Faria Journal: Molecules Date: 2021-04-30 Impact factor: 4.411
Authors: Sebastião Gomes Silva; Renato Araújo da Costa; Mozaniel Santana de Oliveira; Jorddy Neves da Cruz; Pablo Luis B Figueiredo; Davi do Socorro Barros Brasil; Lidiane Diniz Nascimento; Antônio Maia de Jesus Chaves Neto; Raul Nunes de Carvalho Junior; Eloisa Helena de Aguiar Andrade Journal: PLoS One Date: 2019-03-08 Impact factor: 3.240
Authors: Sebastião G Silva; Pablo Luis B Figueiredo; Lidiane D Nascimento; Wanessa A da Costa; José Guilherme S Maia; Eloisa Helena A Andrade Journal: Chem Cent J Date: 2018-11-12 Impact factor: 4.215