BACKGROUND: Very few studies have examined the diversity of human leukocyte antigens (HLA) in the Brazilian renal transplant candidates. METHODS: The frequencies of the HLA-A, HLA-B, and HLA-DRB1 alleles, haplotypes and phenotypes were studied in 522 patients with chronic renal failure, renal transplant candidates, registered at the Transplant Centers in north/northwestern Paraná State, southern Brazil. Patients were classified according to the ethnic group (319 whites [Caucasians], 134 mestizos [mixed race descendants of Europeans, Africans, and Amerindians; browns or "pardos"] and 69 blacks). The HLA typing was performed by the polymerase chain reaction sequence-specific oligonucleotide method (PCR-SSO), combined with Luminex technology. RESULTS: In the analysis of the total samples, 20 HLA-A, 32 HLA-B, and 13 HLA-DRB1 allele groups were identified. The most frequent allele groups for each HLA locus were HLA-A*02 (25.4%), HLA-B*44 (10.9%), and HLA-DRB1*13 (13.9%). The most frequent haplotypes were HLA-A*01-B*08-DRB1*03 (2.3%), A*02-B*44-DRB1*07 (1.2%), and A*03-B*07-DRB1*11 (1.0%). Significant differences (P < 0.05) were observed in the HLA-A*68, B*08, and B*58 allele frequencies among ethnic groups. CONCLUSIONS: This study provides the first data on the HLA-A, HLA-B, and HLA-DRB1 allele, phenotype and haplotype frequencies of renal transplant candidates in a population in southern Brazil.
BACKGROUND: Very few studies have examined the diversity of human leukocyte antigens (HLA) in the Brazilian renal transplant candidates. METHODS: The frequencies of the HLA-A, HLA-B, and HLA-DRB1 alleles, haplotypes and phenotypes were studied in 522 patients with chronic renal failure, renal transplant candidates, registered at the Transplant Centers in north/northwestern Paraná State, southern Brazil. Patients were classified according to the ethnic group (319 whites [Caucasians], 134 mestizos [mixed race descendants of Europeans, Africans, and Amerindians; browns or "pardos"] and 69 blacks). The HLA typing was performed by the polymerase chain reaction sequence-specific oligonucleotide method (PCR-SSO), combined with Luminex technology. RESULTS: In the analysis of the total samples, 20 HLA-A, 32 HLA-B, and 13 HLA-DRB1 allele groups were identified. The most frequent allele groups for each HLA locus were HLA-A*02 (25.4%), HLA-B*44 (10.9%), and HLA-DRB1*13 (13.9%). The most frequent haplotypes were HLA-A*01-B*08-DRB1*03 (2.3%), A*02-B*44-DRB1*07 (1.2%), and A*03-B*07-DRB1*11 (1.0%). Significant differences (P < 0.05) were observed in the HLA-A*68, B*08, and B*58 allele frequencies among ethnic groups. CONCLUSIONS: This study provides the first data on the HLA-A, HLA-B, and HLA-DRB1 allele, phenotype and haplotype frequencies of renal transplant candidates in a population in southern Brazil.
Authors: J J Chen; J A Hollenbach; E A Trachtenberg; J J Just; M Carrington; K S Rønningen; A Begovich; M C King; S McWeeney; S J Mack; H A Erlich; G Thomson Journal: Tissue Antigens Date: 1999-12
Authors: F Williams; A Meenagh; C Darke; A Acosta; A S Daar; C Gorodezky; M Hammond; E Nascimento; D Middleton Journal: Hum Immunol Date: 2001-06 Impact factor: 2.850
Authors: A Piazza; E Olivetti; R M Griffo; S Rendine; A Amoroso; M Barbanti; C Caruso; C Conighi; R Conte; B Favoino Journal: Gene Geogr Date: 1989 Aug-Dec
Authors: Damacio R Kaimen-Maciel; Edna Maria V Reiche; Sueli D Borelli; Helena K Morimoto; Fabiano C Melo; Josiane Lopes; Raffael F Dorigon; Christiane Cavalet; Elton M Yamaguchi; Thiago L Silveira; Waldir V Da Silva; Elizabeth R Comini-Frota; Doralina G Brum Souza; Eduardo A Donadi Journal: Mol Med Rep Date: 2009 Nov-Dec Impact factor: 2.952