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Literature DB >> 2584959

Detection of human coronavirus 229E in nasal washings using RNA:RNA hybridisation.

Abstract

A method is described for the detection of human coronavirus 229E (HCV 229E) in nasal washings using RNA:RNA filter hybridisation. Volunteers were inoculated with HCV 229E, and daily nasal washings were collected. These washings were then examined for the presence of viral RNA using a single-stranded RNA probe. Nucleic acid hybridisation is shown to be a sensitive technique for the diagnosis of HCV 229E infections.

Entities: CellLine Disease Species

Mesh：

Substances：
RNA Probes

Year: 1989 PMID： 2584959 PMCID： PMC7166451 DOI： 10.1002/jmv.1890290113

Source DB: PubMed Journal: J Med Virol ISSN： 0146-6615 Impact factor: 2.327

9 in total

1. Effect of specific humoral immunity and some non-specific factors on resistance of volunteers to respiratory coronavirus infection.

Authors: K A Callow
Journal: J Hyg (Lond) Date: 1985-08

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3. A simple and very efficient method for generating cDNA libraries.

Authors: U Gubler; B J Hoffman
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4. Clones of MRC-C cells may be superior to the parent line for the culture of 229E-like strains of human respiratory coronavirus.

Authors: R J Phillpotts
Journal: J Virol Methods Date: 1983-05 Impact factor: 2.014

5. Epidemiology of coronavirus respiratory infections.

Authors: D Isaacs; D Flowers; J R Clarke; H B Valman; M R MacNaughton
Journal: Arch Dis Child Date: 1983-07 Impact factor: 3.791

6. Diagnosis of human coronavirus infection by immunofluorescence: method and application to respiratory disease in hospitalized children.

Authors: K McIntosh; J McQuillin; S E Reed; P S Gardner
Journal: J Med Virol Date: 1978 Impact factor: 2.327

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Authors: H Riski; T Hovi
Journal: J Med Virol Date: 1980 Impact factor: 2.327

8. Coronaviridae.

Authors: S G Siddell; R Anderson; D Cavanagh; K Fujiwara; H D Klenk; M R Macnaughton; M Pensaert; S A Stohlman; L Sturman; B A van der Zeijst
Journal: Intervirology Date: 1983 Impact factor: 1.763

Review 9. Human enteric coronaviruses. Brief review.

Authors: M R Macnaughton; H A Davies
Journal: Arch Virol Date: 1981 Impact factor: 2.574

9 in total

7 in total

Detection of human coronavirus 229E in nasal washings using RNA:RNA hybridisation.

1. Effect of specific humoral immunity and some non-specific factors on resistance of volunteers to respiratory coronavirus infection.

2. Coronavirus infection in acute lower respiratory tract disease of infants.

3. A simple and very efficient method for generating cDNA libraries.

4. Clones of MRC-C cells may be superior to the parent line for the culture of 229E-like strains of human respiratory coronavirus.

5. Epidemiology of coronavirus respiratory infections.

6. Diagnosis of human coronavirus infection by immunofluorescence: method and application to respiratory disease in hospitalized children.

7. Coronavirus infections of man associated with diseases other than the common cold.

8. Coronaviridae.

Review 9. Human enteric coronaviruses. Brief review.

1. Antigen detection in human respiratory Coronavirus infections by monoclonal time-resolved fluoroimmunoassay.

2. Characterization of a nucleic acid probe for the diagnosis of human coronavirus 229E infections.

3. Coronaviruses in the limelight.

4. Detection of rhinovirus RNA in nasal epithelial cells by in situ hybridization.

5. An adult model of exclusive viral wheeze: inflammation in the upper and lower respiratory tracts.

6. Detection of human coronavirus 229E-specific antibodies using recombinant fusion proteins.

7. Coronavirus 229E-related pneumonia in immunocompromised patients.