Literature DB >> 2167350

Characterization of a nucleic acid probe for the diagnosis of human coronavirus 229E infections.

S Myint1, D Harmsen, T Raabe, S G Siddell.   

Abstract

A cDNA copy of the HCV229E nucleocapsid protein gene was isolated and characterized. Sequence analysis predicts a nucleocapsid polypeptide of 389 amino acids with a molecular weight (mol. wt.) of 43,450. Single strand RNA probes derived from the cDNA copy hybridize specifically to HCV229E RNA and approximately 50 pg of intracellular viral RNA can be readily detected. The application of nucleic acid hybridization as a routine procedure for the diagnosis of HCV229E infection is discussed.

Entities:  

Mesh：

Substances：

Year:  1990        PMID： 2167350      PMCID： PMC7167089          DOI： 10.1002/jmv.1890310216

Source DB:  PubMed          Journal:  J Med Virol        ISSN： 0146-6615            Impact factor:   2.327


  34 in total

1.  A new virus isolated from the human respiratory tract.

Authors:  D Hamre; J J Procknow
Journal:  Proc Soc Exp Biol Med       Date:  1966-01

2.  Cleavage of structural proteins during the assembly of the head of bacteriophage T4.

Authors:  U K Laemmli
Journal:  Nature       Date:  1970-08-15       Impact factor: 49.962

3.  A simple and very efficient method for generating cDNA libraries.

Authors:  U Gubler; B J Hoffman
Journal:  Gene       Date:  1983-11       Impact factor: 3.688

4.  High yield growth and purification of human parainfluenza type 3 virus and initial analysis of viral structural proteins.

Authors:  L E Guskey; G Bergtrom
Journal:  J Gen Virol       Date:  1981-05       Impact factor: 3.891

5.  Automation of the computer handling of gel reading data produced by the shotgun method of DNA sequencing.

Authors:  R Staden
Journal:  Nucleic Acids Res       Date:  1982-08-11       Impact factor: 16.971

6.  Clones of MRC-C cells may be superior to the parent line for the culture of 229E-like strains of human respiratory coronavirus.

Authors:  R J Phillpotts
Journal:  J Virol Methods       Date:  1983-05       Impact factor: 2.014

7.  Two antigenic groups of human coronaviruses detected by using enzyme-linked immunosorbent assay.

Authors:  M R Macnaughton; M H Madge; S E Reed
Journal:  Infect Immun       Date:  1981-09       Impact factor: 3.441

8.  The 5'-end sequence of the murine coronavirus genome: implications for multiple fusion sites in leader-primed transcription.

Authors:  C K Shieh; L H Soe; S Makino; M F Chang; S A Stohlman; M M Lai
Journal:  Virology       Date:  1987-02       Impact factor: 3.616

9.  Diagnosis of human coronavirus infections in children using enzyme-linked immunosorbent assay.

Authors:  M R Macnaughton; D Flowers; D Isaacs
Journal:  J Med Virol       Date:  1983       Impact factor: 2.327

10.  Characterization of leader-related small RNAs in coronavirus-infected cells: further evidence for leader-primed mechanism of transcription.

Authors:  R S Baric; S A Stohlman; M K Razavi; M M Lai
Journal:  Virus Res       Date:  1985-07       Impact factor: 3.303
View more
  9 in total

1.  Detection of rhinovirus in sinus brushings of patients with acute community-acquired sinusitis by reverse transcription-PCR.

Authors:  A Pitkäranta; E Arruda; H Malmberg; F G Hayden
Journal:  J Clin Microbiol       Date:  1997-07       Impact factor: 5.948

2.  Frequency and natural history of rhinovirus infections in adults during autumn.

Authors:  E Arruda; A Pitkäranta; T J Witek; C A Doyle; F G Hayden
Journal:  J Clin Microbiol       Date:  1997-11       Impact factor: 5.948

3.  Murine coronavirus nonstructural protein ns2 is not essential for virus replication in transformed cells.

Authors:  B Schwarz; E Routledge; S G Siddell
Journal:  J Virol       Date:  1990-10       Impact factor: 5.103

4.  Characterization of a human coronavirus (strain 229E) 3C-like proteinase activity.

Authors:  J Ziebuhr; J Herold; S G Siddell
Journal:  J Virol       Date:  1995-07       Impact factor: 5.103

5.  Human coronavirus 229E encodes a single ORF4 protein between the spike and the envelope genes.

Authors:  Ronald Dijkman; Maarten F Jebbink; Berry Wilbrink; Krzysztof Pyrc; Hans L Zaaijer; Philip D Minor; Sally Franklin; Ben Berkhout; Volker Thiel; Lia van der Hoek
Journal:  Virol J       Date:  2006-12-28       Impact factor: 4.099

6.  Antigen detection in human respiratory Coronavirus infections by monoclonal time-resolved fluoroimmunoassay.

Authors:  J C Hierholzer; P E Halonen; P G Bingham; R A Coombs; Y O Stone
Journal:  Clin Diagn Virol       Date:  1994-06

7.  Polymerase chain reaction-based detection of rhinovirus, respiratory syncytial virus, and coronavirus in otitis media with effusion.

Authors:  A Pitkäranta; J Jero; E Arruda; A Virolainen; F G Hayden
Journal:  J Pediatr       Date:  1998-09       Impact factor: 4.406

8.  Detection of human coronavirus 229E-specific antibodies using recombinant fusion proteins.

Authors:  A Pohl-Koppe; T Raabe; S G Siddell; V ter Meulen
Journal:  J Virol Methods       Date:  1995-10       Impact factor: 2.014

Review 9.  A view from the Common Cold Unit.

Authors:  D A Tyrrell
Journal:  Antiviral Res       Date:  1992-06       Impact factor: 5.970
  9 in total

北京卡尤迪生物科技股份有限公司 © 2022-2023.