Denise C Andia1, Aline C Planello2, Danielle Portinho2, Rodrigo A da Silva2, Cristiane R Salmon3, Enilson A Sallum3, Francisco H Nociti Junior3, Ana P de Souza2. 1. Department of Morphology, Division of Histology, School of Dentistry at Piracicaba, University of Campinas, Av. Limeira 901, 13414-018, Piracicaba, São Paulo-SP, Brazil. denise@andia.com.br. 2. Department of Morphology, Division of Histology, School of Dentistry at Piracicaba, University of Campinas, Av. Limeira 901, 13414-018, Piracicaba, São Paulo-SP, Brazil. 3. Department of Prosthodontics and Periodontics, Division of Periodontics, School of Dentistry at Piracicaba, University of Campinas, Piracicaba, São Paulo-SP, Brazil.
Abstract
OBJECTIVES: DNA methylation plays a critical role in the regulation of the transcription of the suppressors of cytokine signaling (SOCS) 1 and SOCS3, which are modulators in the inflammation. We hypothesized that the methylation status of SOCS1, SOCS3, and long interspersed nuclear element (LINE)-1 in gingival tissues previously inflamed would be similar to that found in gingival tissues without clinical inflammation in the period studied. MATERIALS AND METHODS: Laser capture microdissection was performed to isolate epithelial and connective gingival tissues. The groups were comprised by ten patients without history of periodontitis and absence of clinical signs of inflammation in the gingiva during the study (healthy group) and ten patients with history of periodontitis, presenting inflammation in the gingival tissue at the first examination of the study (controlled chronic periodontitis group). The gingival biopsies from the controlled chronic periodontitis group were collected after controlling the inflammation. DNA methylation patterns were analyzed using methylation-specific high-resolution melting and combined bisulfite restriction analysis. RESULTS: DNA methylation levels for SOCS1 and SOCS3 did not differ between groups or tissues; likewise, no differences were observed in total LINE-1 methylation or at specific loci. CONCLUSION: At 3 months following control of inflammation in gingival tissues, the methylation profile of SOCS1, SOCS3, and LINE-1 is similar between connective and epithelial tissues from patients that were previously affected or not by chronic inflammation. CLINICAL RELEVANCE: Clinical results of a successful treatment are observed after inflammation control and the molecular findings illustrate local and general methylation patterns in recovering tissues toward health conditions and might help to understand events that are occurring in oral cells.
OBJECTIVES: DNA methylation plays a critical role in the regulation of the transcription of the suppressors of cytokine signaling (SOCS) 1 and SOCS3, which are modulators in the inflammation. We hypothesized that the methylation status of SOCS1, SOCS3, and long interspersed nuclear element (LINE)-1 in gingival tissues previously inflamed would be similar to that found in gingival tissues without clinical inflammation in the period studied. MATERIALS AND METHODS: Laser capture microdissection was performed to isolate epithelial and connective gingival tissues. The groups were comprised by ten patients without history of periodontitis and absence of clinical signs of inflammation in the gingiva during the study (healthy group) and ten patients with history of periodontitis, presenting inflammation in the gingival tissue at the first examination of the study (controlled chronic periodontitis group). The gingival biopsies from the controlled chronic periodontitis group were collected after controlling the inflammation. DNA methylation patterns were analyzed using methylation-specific high-resolution melting and combined bisulfite restriction analysis. RESULTS: DNA methylation levels for SOCS1 and SOCS3 did not differ between groups or tissues; likewise, no differences were observed in total LINE-1 methylation or at specific loci. CONCLUSION: At 3 months following control of inflammation in gingival tissues, the methylation profile of SOCS1, SOCS3, and LINE-1 is similar between connective and epithelial tissues from patients that were previously affected or not by chronic inflammation. CLINICAL RELEVANCE: Clinical results of a successful treatment are observed after inflammation control and the molecular findings illustrate local and general methylation patterns in recovering tissues toward health conditions and might help to understand events that are occurring in oral cells.
Entities:
Keywords:
DNA methylation; Inflammation; Long interspersed nuclear elements; Suppressor of cytokine Signaling proteins
Authors: Sharon Veenbergen; Miranda B Bennink; Alsya J Affandi; Natacha Bessis; Jérôme Biton; Onno J Arntz; Wim B van den Berg; Fons A J van de Loo Journal: Ann Rheum Dis Date: 2011-08-27 Impact factor: 19.103
Authors: Peter K K Wong; Paul J Egan; Ben A Croker; Kristy O'Donnell; Natalie A Sims; Sarah Drake; Hiu Kiu; Edward J McManus; Warren S Alexander; Andrew W Roberts; Ian P Wicks Journal: J Clin Invest Date: 2006-05-18 Impact factor: 14.808
Authors: Florença Abdanur Stefani; Michelle Beatriz Viana; Ana Carolina Dupim; João Artur Ricieri Brito; Ricardo Santiago Gomez; José Eustáquio da Costa; Paula Rocha Moreira Journal: Immunobiology Date: 2012-12-13 Impact factor: 3.144
Authors: P Isomäki; T Alanärä; P Isohanni; A Lagerstedt; M Korpela; T Moilanen; T Visakorpi; O Silvennoinen Journal: Rheumatology (Oxford) Date: 2007-08-27 Impact factor: 7.580
Authors: Shaoping Zhang; Silvana P Barros; Antonio J Moretti; Ning Yu; Jing Zhou; John S Preisser; Mihai D Niculescu; Steven Offenbacher Journal: J Periodontol Date: 2013-01-31 Impact factor: 6.993
Authors: Ismael Khouly; Simon Pardiñas López; Silvia María Díaz Prado; Luca Ferrantino; Josephine Kalm; Lena Larsson; Farah Asa'ad Journal: Int J Environ Res Public Health Date: 2022-01-17 Impact factor: 3.390