Literature DB >> 2582622

An improved pyrogallol red-molybdate method for determining total urinary protein.

J L Orsonneau1, P Douet, C Massoubre, P Lustenberger, S Bernard.   

Abstract

We adapted the pyrogallol red-molybdate method for total urinary protein to the Cobas Bio centrifugal analyzer. The method is simple, rapid, sensitive, and inexpensive. Addition of 25 mg of sodium dodecyl sulfate per liter to the reagent modifies protein reactivities so that the chromogenicity of human gamma globulins is the same as that of albumin. Results by this method and a comparison method that included gel filtration and a modified biuret reaction correlated well (r = 0.951).

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Year:  1989        PMID: 2582622

Source DB:  PubMed          Journal:  Clin Chem        ISSN: 0009-9147            Impact factor:   8.327


  30 in total

1.  Application of an improved biuret method to the determination of total protein in urine and cerebrospinal fluid without concentration step by use of Hitachi 7170 auto-analyzer.

Authors:  X Guobing; J Lili; Z Lihua; X Tiean
Journal:  J Clin Lab Anal       Date:  2001       Impact factor: 2.352

2.  -11 Mutation in the ampC promoter increasing resistance to beta-lactams in a clinical Escherichia coli strain.

Authors:  S Corvec; N Caroff; E Espaze; J Marraillac; A Reynaud
Journal:  Antimicrob Agents Chemother       Date:  2002-10       Impact factor: 5.191

3.  MCP-1 gene activation marks acute kidney injury.

Authors:  Raj Munshi; Ali Johnson; Edward D Siew; T Alp Ikizler; Lorraine B Ware; Mark M Wurfel; Jonathan Himmelfarb; Richard A Zager
Journal:  J Am Soc Nephrol       Date:  2010-11-11       Impact factor: 10.121

4.  Comparison of Urinary Total Proteins by Four Different Methods.

Authors:  Padma Yalamati; Madhu Latha Karra; Aparna V Bhongir
Journal:  Indian J Clin Biochem       Date:  2016-02-08

5.  Altered activity of plasma hemopexin in patients with minimal change disease in relapse.

Authors:  Winston W Bakker; Catharina M L van Dael; Leonie J W M Pierik; Joanna A E van Wijk; Jeroen Nauta; Theo Borghuis; Jola J Kapojos
Journal:  Pediatr Nephrol       Date:  2005-08-04       Impact factor: 3.714

6.  GlycoFibroTest is a highly performant liver fibrosis biomarker derived from DNA sequencer-based serum protein glycomics.

Authors:  Dieter Vanderschaeghe; Wouter Laroy; Erwin Sablon; Philippe Halfon; Annelies Van Hecke; Joris Delanghe; Nico Callewaert
Journal:  Mol Cell Proteomics       Date:  2009-01-29       Impact factor: 5.911

7.  Risk factors associated with secondary hyperparathyroidism in patients with chronic kidney disease.

Authors:  Yudan Wei; Jing Lin; Fan Yang; Xiujiang Li; Yue Hou; Ronghua Lu; Xiaonv Shi; Zhi Liu; Yujun Du
Journal:  Exp Ther Med       Date:  2016-06-08       Impact factor: 2.447

8.  Salivary antibodies, amylase and protein from children with early childhood caries.

Authors:  Débora Gonçalves de Farias; Ana Cristina Barreto Bezerra
Journal:  Clin Oral Investig       Date:  2003-08-12       Impact factor: 3.573

9.  Ferrotoxicity and its amelioration by endogenous vitamin D in experimental acute kidney injury.

Authors:  Chandrashekar Annamalai; Rajesh N Ganesh; Pragasam Viswanathan
Journal:  Exp Biol Med (Maywood)       Date:  2020-08-02

10.  Chronic administration of eicosapentaenoic acid and docosahexaenoic acid as ethyl esters reduced plasma cholesterol and changed the fatty acid composition in rat blood and organs.

Authors:  L Frøyland; H Vaagenes; D K Asiedu; A Garras; O Lie; R K Berge
Journal:  Lipids       Date:  1996-02       Impact factor: 1.880

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