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Literature DB >> 2581556

A new, rapid, method for preparation of dispersed pancreatic acini.

R Bruzzone, P A Halban, A Gjinovci, E R Trimble.

Abstract

A new method for the preparation of pancreatic acini is described. The method is simple and much more rapid than previously described techniques, the time required for preparation of pancreatic acini being 20 min from removal of the pancreas. Acini prepared with this method perform in a superior manner when stimulated by either caerulein or secretin. Thus this new technique would be ideal for use in binding and secretion studies.

Entities: Disease Gene

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Year: 1985 PMID： 2581556 PMCID： PMC1144753 DOI： 10.1042/bj2260621

Source DB: PubMed Journal: Biochem J ISSN： 0264-6021 Impact factor: 3.857

15 in total

1. Isolation of pancreatic islets of Langerhans by filtration on nylon mesh.

Authors: R E Offord; P A Halban
Journal: Biochem Biophys Res Commun Date: 1978-06-29 Impact factor: 3.575

Review 2. Preparation of isolated rat liver cells.

Authors: P O Seglen
Journal: Methods Cell Biol Date: 1976 Impact factor: 1.441

3. Interaction of cholecystokinin with specific membrane receptors on pancreatic acinar cells.

Authors: R T Jensen; G F Lemp; J D Gardner
Journal: Proc Natl Acad Sci U S A Date: 1980-04 Impact factor: 11.205

4. Structural and functional characterization of isolated pancreatic exocrine cells.

Authors: A Amsterdam; J D Jamieson
Journal: Proc Natl Acad Sci U S A Date: 1972-10 Impact factor: 11.205

5. Method for the isolation of intact islets of Langerhans from the rat pancreas.

Authors: P E Lacy; M Kostianovsky
Journal: Diabetes Date: 1967-01 Impact factor: 9.461

Review 6. Identification and characterization of receptors for secretagogues on pancreatic acinar cells.

Authors: R T Jensen; J D Gardner
Journal: Fed Proc Date: 1981-08

7. Kinetics of amylase release by dispersed acini prepared from guinea pig pancreas.

Authors: S R Peikin; A J Rottman; S Batzri; J D Gardner
Journal: Am J Physiol Date: 1978-12

8. Calcium uptake into acini from rat pancreas: evidence for intracellular ATP-dependent calcium sequestration.

Authors: H Wakasugi; T Kimura; W Haase; A Kribben; R Kaufmann; I Schulz
Journal: J Membr Biol Date: 1982 Impact factor: 1.843

9. Glucose-insulin interactions on exocrine secretion from the perfused rat pancreas.

Authors: R Bruzzone; E R Trimble; A Gjinovci; A E Renold
Journal: Gastroenterology Date: 1984-12 Impact factor: 22.682

10. Studies on dispersed pancreatic exocrine cells. I. Dissociation technique and morphologic characteristics of separated cells.

Authors: A Amsterdam; J D Jamieson
Journal: J Cell Biol Date: 1974-12 Impact factor: 10.539

33 in total

10. Agonist-sensitive and -insensitive intracellular Ca2+ pools. Separate Ca(2+)-releasing mechanisms revealed by manoalide and benzohydroquinone.

Authors: S Muallem; P Loessberg; G Sachs; L A Wheeler
Journal: Biochem J Date: 1991-10-15 Impact factor: 3.857

A new, rapid, method for preparation of dispersed pancreatic acini.

1. Isolation of pancreatic islets of Langerhans by filtration on nylon mesh.

Review 2. Preparation of isolated rat liver cells.

3. Interaction of cholecystokinin with specific membrane receptors on pancreatic acinar cells.

4. Structural and functional characterization of isolated pancreatic exocrine cells.

5. Method for the isolation of intact islets of Langerhans from the rat pancreas.

Review 6. Identification and characterization of receptors for secretagogues on pancreatic acinar cells.

7. Kinetics of amylase release by dispersed acini prepared from guinea pig pancreas.

8. Calcium uptake into acini from rat pancreas: evidence for intracellular ATP-dependent calcium sequestration.

9. Glucose-insulin interactions on exocrine secretion from the perfused rat pancreas.

10. Studies on dispersed pancreatic exocrine cells. I. Dissociation technique and morphologic characteristics of separated cells.

1. Actin coating of secretory granules during regulated exocytosis correlates with the release of rab3D.

2. Multiple isoforms of the ryanodine receptor are expressed in rat pancreatic acinar cells.

3. On the protective mechanisms of nitric oxide in acute pancreatitis.

4. Caerulein and carbamoylcholine stimulate pancreatic amylase release at resting cytosolic free Ca2+.

5. Gap junctional coupling modulates secretion of exocrine pancreas.

6. Two components of hormone-evoked calcium release from intracellular stores of pancreatic acinar cells.

7. The action of the cholecystokinin-A receptor antagonist, devazepide, on the digestive system of the chicken.

8. A membrane lipid imbalance plays a role in the phenotypic expression of cystic fibrosis in cftr(-/-) mice.

9. Mammalian Sec23p homologue is restricted to the endoplasmic reticulum transitional cytoplasm.

10. Agonist-sensitive and -insensitive intracellular Ca2+ pools. Separate Ca(2+)-releasing mechanisms revealed by manoalide and benzohydroquinone.