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Literature DB >> 2581474

Blot analyses of glycoconjugates: enzyme-hydrazide--a novel reagent for the detection of aldehydes.

Abstract

A procedure for the general staining of glycoproteins and other glycoconjugates on protein blots has been developed. Aldehydes are formed on the sugars of glycoconjugates by periodate oxidation which then react with hydrazide groups of enzyme-hydrazides, a novel reagent designed for aldehyde detection. The bound enzyme-hydrazide is demonstrated histochemically. The new assay is advantageous over periodic-acid Schiff staining of gels as its reagents and signals are stable and the process is simple and expedient, and provides greater sensitivity.

Entities: Chemical

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Year: 1985 PMID： 2581474 DOI： 10.1016/0003-2697(85)90395-1

Source DB: PubMed Journal: Anal Biochem ISSN： 0003-2697 Impact factor: 3.365

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Cited

12 in total

1. A major barley allergen associated with baker's asthma disease is a glycosylated monomeric inhibitor of insect alpha-amylase: cDNA cloning and chromosomal location of the gene.

Authors: M Mena; R Sanchez-Monge; L Gomez; G Salcedo; P Carbonero
Journal: Plant Mol Biol Date: 1992-11 Impact factor: 4.076

2. Preparation of the stabilized glycoenzymes by cross-linking their carbohydrate chains.

Authors: B Kozulić; I Leustek; B Pavlović; P Mildner; S Barbarić
Journal: Appl Biochem Biotechnol Date: 1987-10 Impact factor: 2.926

3. Identification and partial purification of ABGP205, an integral membrane glycoprotein from brain that binds ankyrin.

Authors: K J Treharne; D Rayner; A J Baines
Journal: Biochem J Date: 1988-07-15 Impact factor: 3.857

4. A glycoprotein multimer from Bacillus thuringiensis sporangia: dissociation into subunits and sugar composition.

Authors: M García-Patrone; J S Tandecarz
Journal: Mol Cell Biochem Date: 1995-04-12 Impact factor: 3.396

5. Metabolic regulation by C1q/TNF-related protein-13 (CTRP13): activation OF AMP-activated protein kinase and suppression of fatty acid-induced JNK signaling.

Authors: Zhikui Wei; Jonathan M Peterson; G William Wong
Journal: J Biol Chem Date: 2011-03-04 Impact factor: 5.157

6. Mammalian Otolin: a multimeric glycoprotein specific to the inner ear that interacts with otoconial matrix protein Otoconin-90 and Cerebellin-1.

Authors: Michael R Deans; Jonathan M Peterson; G William Wong
Journal: PLoS One Date: 2010-09-15 Impact factor: 3.240

Blot analyses of glycoconjugates: enzyme-hydrazide--a novel reagent for the detection of aldehydes.

1. A major barley allergen associated with baker's asthma disease is a glycosylated monomeric inhibitor of insect alpha-amylase: cDNA cloning and chromosomal location of the gene.

2. Preparation of the stabilized glycoenzymes by cross-linking their carbohydrate chains.

3. Identification and partial purification of ABGP205, an integral membrane glycoprotein from brain that binds ankyrin.

4. A glycoprotein multimer from Bacillus thuringiensis sporangia: dissociation into subunits and sugar composition.

5. Metabolic regulation by C1q/TNF-related protein-13 (CTRP13): activation OF AMP-activated protein kinase and suppression of fatty acid-induced JNK signaling.

6. Mammalian Otolin: a multimeric glycoprotein specific to the inner ear that interacts with otoconial matrix protein Otoconin-90 and Cerebellin-1.

7. C1q/tumor necrosis factor-related protein 11 (CTRP11), a novel adipose stroma-derived regulator of adipogenesis.

8. Biotin binding to avidin. Oligosaccharide side chain not required for ligand association.

9. Modifications of plasma fibronectin in cystic fibrosis patients.

10. Purification and properties of sialoadhesin, a sialic acid-binding receptor of murine tissue macrophages.