Hao Cai1, Gang Cao2,3, Hong-Yan Zhang4. 1. School of Pharmacy, Nanjing University of Chinese Medicine, Nanjing, 210023, China. 2. School of Pharmacy, Nanjing University of Chinese Medicine, Nanjing, 210023, China. caogang33@163.com. 3. Research Center of Traditional Chinese Medicine Processing Technology, Zhejiang Chinese Medical University, Hangzhou, 310053, China. caogang33@163.com. 4. Zhejiang Cancer Hospital, Hangzhou, 310022, China.
Abstract
OBJECTIVE: To investigate the chemical transformation of volatile compounds in sulfur-fumigated Radix Angelicae Sinensis. METHODS: A comprehensive two-dimensional gas chromatography (GC×GC) and high-resolution time-of-flight mass spectrometry (HR-TOF/MS) with colorized fuzzy difference (CFD) method was used to investigate the effect of sulfur-fumigation on the volatile components from Radix Angelicae Sinensis. RESULTS: Twenty-five compounds that were found in sun-dried samples disappeared in sulfur-fumigated samples. Seventeen volatile components including two sulfur-containing compounds were newly generated for the first time in volatile oils of sulfur-fumigated Radix Angelicae Sinensis. CONCLUSION: The strategy can be successfully applied to rapidly and holistically discriminate sun-dried and sulfur-fumigated Radix Angelicae Sinensis. GC×GC-HR-TOF/MS based CFD is a powerful and feasible approach for the global quality evaluation of Radix Angelicae Sinensis as well as other herbal medicines.
OBJECTIVE: To investigate the chemical transformation of volatile compounds in sulfur-fumigated Radix Angelicae Sinensis. METHODS: A comprehensive two-dimensional gas chromatography (GC×GC) and high-resolution time-of-flight mass spectrometry (HR-TOF/MS) with colorized fuzzy difference (CFD) method was used to investigate the effect of sulfur-fumigation on the volatile components from Radix Angelicae Sinensis. RESULTS: Twenty-five compounds that were found in sun-dried samples disappeared in sulfur-fumigated samples. Seventeen volatile components including two sulfur-containing compounds were newly generated for the first time in volatile oils of sulfur-fumigated Radix Angelicae Sinensis. CONCLUSION: The strategy can be successfully applied to rapidly and holistically discriminate sun-dried and sulfur-fumigated Radix Angelicae Sinensis. GC×GC-HR-TOF/MS based CFD is a powerful and feasible approach for the global quality evaluation of Radix Angelicae Sinensis as well as other herbal medicines.
Entities:
Keywords:
Radix Angelicae Sinensis; colorized fuzzy difference; comprehensive two-dimensional gas chromatography and high-resolution time-of-flight mass spectrometry; volatile components
Authors: Qing-Feng Xie; Jian-Hui Xie; Tina T X Dong; Ji-Yan Su; Da-Ke Cai; Jian-Ping Chen; Liang-Feng Liu; Yu-Cui Li; Xiao-Ping Lai; Karl W K Tsim; Zi-Ren Su Journal: J Ethnopharmacol Date: 2012-10-02 Impact factor: 4.360
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