| Literature DB >> 25800832 |
Ryan M Nottingham1, Suzanne R Pfeffer.
Abstract
Mammalian cells encode a diverse set of Rab GTPases and their corresponding regulators. In vitro biochemical screening has proven invaluable in assigning particular Rabs as substrates for their cognate GTPase-activating proteins. However, in vitro activity does not always reflect substrate specificity in cells. This method describes a functional test of GAP activity in cells or extracts that takes into account the presence of other factors or conditions that might change observed in vitro specificity.Entities:
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Year: 2015 PMID： 25800832 PMCID： PMC4710138 DOI： 10.1007/978-1-4939-2569-8_5
Source DB: PubMed Journal: Methods Mol Biol ISSN： 1064-3745