| Literature DB >> 25799200 |
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Abstract
Entities:
Year: 2015 PMID: 25799200 PMCID: PMC4370458 DOI: 10.1371/journal.pone.0121469
Source DB: PubMed Journal: PLoS One ISSN: 1932-6203 Impact factor: 3.240
Number of tested chicken meat product samples, detected Salmonella isolates, resistant isolates, and resistance genes by year.
| Isolation year | 1996 | 1997 | 1998 | 1999 | 2000 | 2001 | 2002 | 2003 |
|---|---|---|---|---|---|---|---|---|
| No. of samples tested | 41 | 21 | 34 | 34 | 35 | 36 | 33 | 39 |
| No. of | 16 | 9 | 6 | 4 | 20 | 16 | 14 | 21 |
| (%) | (39.0%) | (42.9%) | (17.6%) | (11.8%) | (57.1%) | (44.4%) | (42.4%) | (53.8%) |
| No. of | 16 | 12 | 6 | 5 | 20 | 16 | 14 | 21 |
| No. of samples from which multiple | 0 | 3 | 0 | 1 | 0 | 0 | 0 | 0 |
|
| Corvallis (1) | Agona (1), | Corvallis (3), | Infantis (3), | Corvallis (1), | Haifa (2), | Infantis (12), | Cerro (1), |
| Haifa (1), | Corvallis (1), | Enteritidis (1), | Untypeable with O7 (1), | Infantis (18), | Infantis (8), | Yovokome (1), | Haifa (1), | |
| Infantis (12), | Haifa (1), | Infantis (1), | Untypeable (1) | Untypeable with O7 (1) | Virchow (2), | Untypeable with O7:y:- (1) | Infantis (14), | |
| Typhimurium (1), | Infantis (5), | Typhimurium (1) | Untypeable with O18: Z4, Z23: —(3), | Manhattan (2), | ||||
| Untypeable with O4:i: (1) | Typhimurium (2), | Untypeable with O7 (1) | Untypeable (3) | |||||
| Untypeable with O4:b:- (1), | ||||||||
| Untypeable with O8:z4,z24 (1) | ||||||||
| Untested isolates with antimicrobial susceptibility | - | - | - | - | - | - | - | - |
| No. of tested isolates | 16 | 12 | 6 | 5 | 20 | 16 | 14 | 21 |
| (No. of samples) | (16) | (9) | (6) | (4) | (20) | (16) | (14) | (21) |
| No. of resistant isolates | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
| (Percentage of tested isolates) | (0.0%) | (0.0%) | (0.0%) | (0.0%) | (0.0%) | (0.0%) | (0.0%) | (0.0%) |
| Detected resistance genes and serotypes | - | - | - | - | - | - | - | - |
| Isolation year | 2004 | 2005 | 2006 | 2007 | 2008 | 2009 | 2010 | Total |
| No. of samples tested | 35 | 40 | 40 | 40 | 157 | 88 | 106 | 779 |
| No. of | 13 | 22 | 13 | 22 | 76 | 48 | 55 | 355 |
| (%) | (37.1%) | (55.0%) | (32.5%) | (55.0%) | (48.4%) | (54.5%) | (51.9%) | (45.6%) |
| No. of | 13 | 25 | 14 | 24 | 81 | 50 | 61 | 378 |
| No. of samples from which multiple | 0 | 3 | 1 | 2 | 4 | 2 | 6 | 22 |
|
| Enteritidis (1), | Enteritidis (1), | Dunkwa (1), | Corvallis (1), | Enteritidis (1), | Enteritidis (1), | Agona (1), | Infantis (180), |
| Infantis (9), | Haifa (1), | Infantis (8), | Emek (1), | Eppendorf (2), | Infantis (14), | Infantis (20), | Schwarzengrund (70), | |
| Manhattan (1), | Corvallis (1), | Manhattan (3), | Enteritidis (1), | Infantis (33), | Manhattan (8), | Manhattan (19), | Manhattan (52), | |
| Yovokome (1), | Infantis (15), | Schwarzengrund (2) | Eppendorf (1), | Jamaica (1), | Schwarzengrund (21), | Schwarzengrund (18), | Untypeable (11), | |
| Untypeable (1) | Manhattan (2), | Infantis (8), | Manhattan (15), | Untypeable with O4 (3), | Virchow (1), | Corvallis (8), | ||
| Montevideo (1), | Manhattan (2), | Schwarzengrund (21), | Untypeable (3) | Untypeable with O4 (1), | Enteritidis (6), | |||
| Schwarzengrund (1), | Schwarzengrund (7), | Untypeable with O-untypeable:r:1,5 (3), | Untypeable (1) | Haifa (6), | ||||
| Typhimurium (1), | Typhimurium (1), | Unrecorded (5) | Typhimurium (6), | |||||
| Untypeable (2) | Untypeable with O4 (1), | Unrecorded (5), | ||||||
| Untypeable with O7 (1) | and other serotypes | |||||||
| Untested isolates with antimicrobial susceptibility | - | - | - | - | Unrecorded (5) | - | - | 5 |
| No. of tested isolates | 13 | 25 | 14 | 24 | 76 | 50 | 61 | 373 |
| (No. of samples) | (13) | (22) | (13) | (22) | (71) | (48) | (55) | (350) |
| No. of resistant isolates | 0 | 2 | 0 | 1 | 8 | 7 | 17 | 35 |
| (Percentage of tested isolates) | (0.0%) | (8.0%) | (0.0%) | (4.2%) | (10.5%) | (14.0%) | (27.9%) | (9.4%) |
| Detected resistance genes and serotypes | - | CMY-2 (Infantis, n = 1) | - | CMY-2 (Infantis, n = 1) | CMY-2 (Infantis, n = 5), | CMY-2 (Infantis, n = 6), | CMY-2 (Infantis, n = 11), | CMY-2 (Infantis, n = 24), |
| TEM-20 (Infantis, n = 1) | CMY-2 (O-untypeable:r:1,5, n = 1), | TEM-52 (Manhattan, n = 1) | CMY-2 (Manhattan, n = 1), | CTX-M-15 & TEM-1 (Manhattan, n = 2), | ||||
| TEM-52 (Infantis, n = 1), | TEM-52 (Manhattan, n = 1), | TEM-52 (Manhattan, n = 2), | ||||||
| CTX-M-2 (Manhattan, n = 1) | CTX-M-2 (Infantis, n = 1) | CMY-2 (Manhattan, n = 1), | ||||||
| CTX-M-15 & TEM-1 (Manhattan, n = 2), | CMY-2 (O-untypeable:r:1,5, n = 1), | |||||||
| SHV-12 (Manhattan, n = 1) | CTX-M-2 (Infantis, n = 1), | |||||||
| CTX-M-2 (Manhattan, n = 1), | ||||||||
| SHV-12 (Manhattan, n = 1), | ||||||||
| TEM-20 (Infantis, n = 1), | ||||||||
| TEM-52 (Infantis, n = 1) |
*Corvallis, Salmonella enterica subsp. enterica serovar Corvallis.
†One sample contained three serotypes, while other multi-Salmonella samples contained two serotypes.
‡Other serotypes, untypeable with O4 (5), untypeable with O7 (4), Eppendorf (3), untypeable with O18: Z4, Z23: —(3), untypeable with O-untypeable:r:1,5 (3), Virchow (3), Agona (2), Yovokome (2), Cerro (1), Dunkwa (1), Emek (1), Jamaica (1), Montevideo (1), untypeable with O4:b:- (1), untypeable with O4:i: (1), untypeable with O7:y:- (1), and untypeable with O8:z4,z24 (1).
§CMY-2, bla CMY-2.
Sequences of primers used in this study.
|
| Purpose | Primer Name | Nucleotide Sequence 5′–3′ | Reference |
|---|---|---|---|---|
|
| Detection | cmy-F | GACAGCCTCTTTCTCCACA | [22] |
| cmy-R | TGGAACGAAGGCTACGTA | [22] | ||
| Sequencing | CMY2-outF | GTTACAATGTGTGAGAAGCAGTC | This study | |
| CMY2-outR | ATGGGATTTTCCTTGCTGTA | This study | ||
| CMY2-R0 | CAGTATTTCGTGACCGGA | This study | ||
| CMY2-F3 | CTGGATTACGGTTCCGCA | This study | ||
|
| Detection and Sequencing | CTX-MU1 | ATGTGCAGYACCAGTAARGT | [23] |
| CTX-MU2 | TGGGTRAARTARGTSACCAGA | [23] | ||
| Sequencing |
| CTTCCAGAATAAGGAATCCC | [26] | |
|
| CGTCTAAGGCGATAAACAAA | [26] | ||
| CTX-outF2 | GCCAAGGGATAATACTAATAGAGG | This study | ||
| CTX-outR | GCGGAATGATAGAAAGAGATGAG | This study | ||
| CTX-F2 | ACAATACTGCCATGAATAAGCTG | This study | ||
| CTX-R0 | CAATCAGCTTATTCATGGCA | This study | ||
|
| Detection | MAb/F | GGGGAGCTCATAAAATTCTTGAAGAC | [24] |
| MAb/R | GGGGGATCCTTACCAATGCTTAATCA | [24] | ||
| Sequencing | MAb-F2 | AGCCCTCCCGTATCGTAGTT | This study | |
| MAb-F1 | GAGGACCGAAGGAGCTAACC | This study | ||
| MAb-outR | AACTACGATACGGGAGGGCT | This study | ||
|
| Detection | SHV-F | AGGATTGACTGCCTTTTTG | [25] |
| SHV-R | ATTTGCTGATTTCGCTCG | [25] | ||
| Sequencing | SHV-forw | CAAAACGCCGGGTTATTC | [27] | |
| SHV-rev | TTAGCGTTGCCAGTGCT | [27] | ||
| SHVseq-forw | GGATTGACTGCCTTTTTGC | [27] | ||
| SHVseq-rev | GCAAAAAGGCAGTCAATCC | [27] |