Literature DB >> 25797787

Cell membrane integrity and distinguishing between metabolically active and inactive cells as a means of improving viability PCR.

Francesc Codony1, Gemma Agustí2, Anna Allué-Guardia3.   

Abstract

Viability PCR uses cell membrane integrity to differentiate live cells from dead. Our new approach improves viability PCR by enabling it to also discriminate between cells with an intact cell membrane and the ability to actively maintain bacterial homeostasis and cells that have an intact membrane but are metabolically inactive.
Copyright © 2015 Elsevier Ltd. All rights reserved.

Keywords:  Ethidium monoazide; Propidium monoazide; Viability PCR

Mesh：

Substances：

Year:  2015        PMID： 25797787     DOI： 10.1016/j.mcp.2015.03.003

Source DB:  PubMed          Journal:  Mol Cell Probes        ISSN： 0890-8508            Impact factor:   2.365


  9 in total

1.  False-Positive Viability PCR Results: An Association with Microtubes.

Authors:  Gemma Agustí; Mariana Fittipaldi; Francesc Codony
Journal:  Curr Microbiol       Date:  2017-02-07       Impact factor: 2.188

2.  Changes in physiological states of Salmonella Typhimurium measured by qPCR with PMA and DyeTox13 Green Azide after pasteurization and UV treatment.

Authors:  Liyan Li; Jing Fu; Sungwoo Bae
Journal:  Appl Microbiol Biotechnol       Date:  2022-03-09       Impact factor: 4.813

Review 3.  Determination of viable legionellae in engineered water systems: Do we find what we are looking for?

Authors:  Alexander K T Kirschner
Journal:  Water Res       Date:  2016-02-12       Impact factor: 11.236

4.  Improved sample treatment protocol for accurate detection of live Salmonella spp. in food samples by viability PCR.

Authors:  Mai Dinh Thanh; Gemma Agustí; Anneluise Mader; Bernd Appel; Francesc Codony
Journal:  PLoS One       Date:  2017-12-12       Impact factor: 3.240

5.  Monitoring Viable Cells of the Biological Control Agent Lactobacillus plantarum PM411 in Aerial Plant Surfaces by Means of a Strain-Specific Viability Quantitative PCR Method.

Authors:  Núria Daranas; Anna Bonaterra; Jesús Francés; Jordi Cabrefiga; Emilio Montesinos; Esther Badosa
Journal:  Appl Environ Microbiol       Date:  2018-05-01       Impact factor: 4.792

6.  Screening and identification of BP100 peptide conjugates active against Xylella fastidiosa using a viability-qPCR method.

Authors:  Aina Baró; Esther Badosa; Laura Montesinos; Lidia Feliu; Marta Planas; Emilio Montesinos; Anna Bonaterra
Journal:  BMC Microbiol       Date:  2020-07-29       Impact factor: 3.605

7.  Detection and Quantification Methods for Viable but Non-culturable (VBNC) Cells in Process Wash Water of Fresh-Cut Produce: Industrial Validation.

Authors:  Pilar Truchado; Maria I Gil; Mar Larrosa; Ana Allende
Journal:  Front Microbiol       Date:  2020-05-04       Impact factor: 5.640

8.  Droplet Digital PCR Is an Improved Alternative Method for High-Quality Enumeration of Viable Probiotic Strains.

Authors:  Sarah J Z Hansen; Peipei Tang; Anthony Kiefer; Kevin Galles; Connie Wong; Wesley Morovic
Journal:  Front Microbiol       Date:  2020-01-22       Impact factor: 5.640

9.  Absolute Enumeration of Probiotic Strains Lactobacillus acidophilus NCFM® and Bifidobacterium animalis subsp. lactis Bl-04 ® via Chip-Based Digital PCR.

Authors:  Sarah J Z Hansen; Wesley Morovic; Martha DeMeules; Buffy Stahl; Connie W Sindelar
Journal:  Front Microbiol       Date:  2018-04-11       Impact factor: 5.640
  9 in total

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