Eyyup Sabri Seyhanli1, Ugur Lok2, Umut Gulacti2, Hasan Buyukaslan3, Metin Atescelik4, Mustafa Yildiz4, Mehmet Ruhi Onur5, Mehmet Cagri Goktekin6, Suleyman Aydın7. 1. Department of Emergency, Sanliurfa Balikligol State Hospital Sanliurfa, Turkey. 2. Department of Emergency Medicine, Adiyaman University Medical Faculty Adiyaman, Turkey. 3. Department of Emergency Medicine, Harran University Medical Faculty Sanliurfa, Turkey. 4. Department of Emergency Medicine, Firat University Medical Faculty Elazig, Turkey. 5. Department of Radiology, Firat University Medical Faculty Elazig, Turkey. 6. Department of Emergency, Diyarbakir Education and Research Hospital Diyarbakir, Turkey. 7. Department of Clinical Biochemistry, Firat University Medical Faculty Elazig, Turkey.
Abstract
INTRODUCTION: Ghrelin is a novel brain-gut peptide hormone consisted of 28 amino-acid. In the plasma, it exists in two major molecular forms, acylated and des-acyled ghrelin, filtered in glomeruli or secreted by nephrons. Primary biological effects of hormones are regulating appetite, foods intake and energy metabolism. We investigated the changing and relationships between serum and urine ghrelin levels in acute stroke patients to provide more information whether diagnostic parameter. METHODS: Thirty acute stroke patients and thirty consecutive volunteers included in study prospectively. To analyze serum and urine ghrelin levels, at the time of diagnose, all of participant blood and fresh urine (1 ml serum, 2 ml urine respectively) samples were obtained. Serum ghrelin levels analyzed ELISA technique, and urine ghrelin levels studied by validation technique. To compare quantitative data student's t test, and for qualitative data chi-square and Fisher's Exact Chi-square test was used. P<0.05 was considered statistically significant. RESULTS: Urine acyl ghrelin levels found statistically significant between patient and control groups (P=0.001), but there were no statistically significant differences between both groups (P>0.05) in serum acyl gherelin, des-acyl ghrelin and urine des-acyle ghrelin levels. CONCLUSIONS: The results indicate that urine acyl ghrelin levels may be considered as a diagnostic parameter in acute ischemic stroke patients. Further studies delineating the mechanism of these observed results are warranted.
INTRODUCTION:Ghrelin is a novel brain-gut peptide hormone consisted of 28 amino-acid. In the plasma, it exists in two major molecular forms, acylated and des-acyled ghrelin, filtered in glomeruli or secreted by nephrons. Primary biological effects of hormones are regulating appetite, foods intake and energy metabolism. We investigated the changing and relationships between serum and urine ghrelin levels in acute strokepatients to provide more information whether diagnostic parameter. METHODS: Thirty acute strokepatients and thirty consecutive volunteers included in study prospectively. To analyze serum and urine ghrelin levels, at the time of diagnose, all of participant blood and fresh urine (1 ml serum, 2 ml urine respectively) samples were obtained. Serum ghrelin levels analyzed ELISA technique, and urine ghrelin levels studied by validation technique. To compare quantitative data student's t test, and for qualitative data chi-square and Fisher's Exact Chi-square test was used. P<0.05 was considered statistically significant. RESULTS: Urine acyl ghrelin levels found statistically significant between patient and control groups (P=0.001), but there were no statistically significant differences between both groups (P>0.05) in serum acyl gherelin, des-acyl ghrelin and urine des-acyle ghrelin levels. CONCLUSIONS: The results indicate that urine acyl ghrelin levels may be considered as a diagnostic parameter in acute ischemic strokepatients. Further studies delineating the mechanism of these observed results are warranted.
Authors: Ema Kantorova; Maria Chomova; Egon Kurca; Stefan Sivak; Kamil Zelenak; Pavol Kučera; Peter Galajda Journal: Neuro Endocrinol Lett Date: 2011 Impact factor: 0.765
Authors: S Krajewski; M Krajewska; L M Ellerby; K Welsh; Z Xie; Q L Deveraux; G S Salvesen; D E Bredesen; R E Rosenthal; G Fiskum; J C Reed Journal: Proc Natl Acad Sci U S A Date: 1999-05-11 Impact factor: 11.205