Makoto Komura1, Hiroko Komura2, Yushi Otani2, Keisuke Suzuki3, Ryosuke Satake3, Tetsuro Kodaka3, Kan Terawaki3, Hironobu Yonekawa3, Kenichi Ikebukuro3, Kazuto Hoshi4, Tsuyoshi Takato4, Yasuhiko Tabata5, Hiroaki Komuro2, Tadashi Iwanaka2. 1. The department of pediatric surgery, Graduate school of medicine, University of Tokyo, 7-3-1 Hongo, Bunkyou-ku, Tokyo 113-8655 Japan; Department of Pediatrics Surgery, Graduate school of medicine, Saitama Medical University, 38Morohongo, Moroyama-cho, Iruma-gun, Saitama 350-0495 Japan. Electronic address: komura-tky@umin.ac.jp. 2. The department of pediatric surgery, Graduate school of medicine, University of Tokyo, 7-3-1 Hongo, Bunkyou-ku, Tokyo 113-8655 Japan. 3. Department of Pediatrics Surgery, Graduate school of medicine, Saitama Medical University, 38Morohongo, Moroyama-cho, Iruma-gun, Saitama 350-0495 Japan. 4. Department of Tissue Engineering, Graduate school of medicine, University of Tokyo, 7-3-1 Hongo, Bunkyou-ku, Tokyo 113-8655 Japan. 5. Department of Biomaterials, Field of Tissue Engineering, Institute of Frontier Medical Science, Kyoto University, 53 Kawara-cho, Shogoin, Sakyo-ku, Kyoto 606-8507, Japan.
Abstract
PURPOSE: Our objective was to investigate the feasibility of engineering cartilage on the esophagus layer and outside the esophagus. Moreover, we investigated the feasibility of tracheoplasty with cartilage engineered on the esophagus in rabbits. METHODS: Chondrocytes were isolated from auricular cartilages. 1. Engineered cartilage formation by histological findings on/into the esophageal layer was compared with that of injectable scaffold and preformed scaffold with chondrocytes. 2. Chondrocytes adhered to gelatin+vicryl mesh™ and b-FGF, were implanted on the outer esophageal surface. Four weeks after seeding, we found that cartilage was implanted in the midposterior portion of the cervical trachea (n=5), and it was retrieved 8weeks after seeding. RESULTS: 1. A gelatin sponge incorporating β-TCP with vicryl mesh™ showed the best performance for fabricating engineered cartilage on the outer side of the esophagus. 2. Two of 5 rabbits died due to obstructed esophagus. Cartilage engineered outside the esophagus by a composite scaffold as the main material in the gelatin sponge, maintained the airway structure for up to 1month after implantation. Tracheal epithelial regeneration occurred in the internal lumen of this engineered cartilage. CONCLUSION: Tracheoplasty with cartilage engineered outside the esophagus may be useful for reconstructing airways.
PURPOSE: Our objective was to investigate the feasibility of engineering cartilage on the esophagus layer and outside the esophagus. Moreover, we investigated the feasibility of tracheoplasty with cartilage engineered on the esophagus in rabbits. METHODS: Chondrocytes were isolated from auricular cartilages. 1. Engineered cartilage formation by histological findings on/into the esophageal layer was compared with that of injectable scaffold and preformed scaffold with chondrocytes. 2. Chondrocytes adhered to gelatin+vicryl mesh™ and b-FGF, were implanted on the outer esophageal surface. Four weeks after seeding, we found that cartilage was implanted in the midposterior portion of the cervical trachea (n=5), and it was retrieved 8weeks after seeding. RESULTS: 1. A gelatin sponge incorporating β-TCP with vicryl mesh™ showed the best performance for fabricating engineered cartilage on the outer side of the esophagus. 2. Two of 5 rabbits died due to obstructed esophagus. Cartilage engineered outside the esophagus by a composite scaffold as the main material in the gelatin sponge, maintained the airway structure for up to 1month after implantation. Tracheal epithelial regeneration occurred in the internal lumen of this engineered cartilage. CONCLUSION: Tracheoplasty with cartilage engineered outside the esophagus may be useful for reconstructing airways.
Authors: Elizabeth F Maughan; Robert E Hynds; Toby J Proctor; Sam M Janes; Martin Elliott; Martin A Birchall; Mark W Lowdell; Paolo De Coppi Journal: Curr Stem Cell Rep Date: 2017-10-26