INTRODUCTION: Ticks (Acari: Ixodida) are vectors of many pathogens i. a: Babesia microti [Bm] and Toxoplasma gondii [Tg]. In Poland, Ixodes ricinus [Ir] is the main vector of both pathogens. This tick species and pathogens transmitted by them are a significant threat to human and animal health. OBJECTIVES OF THE STUDY: Detection of the protozoans Bm and Tg in ticks collected in the Tarnogórski district area. MATERIAL AND METHODS: The ticks were collected from vegetation and pets in the spring period of their activity on the territory of the Tarnogórski district. The parasites were preserved in 70% ethanol. DNA was isolated by ammonia method. Bm was detected by nested-PCR using specific primers for the 18S rRNA sequence. To detect the Tg a commercial kit was used. The PCR products were separated on 2% ethidium bromide stained agarose gels and visualised under UV light. RESULTS: It was showed that all collected ticks belonged to the species Ir. Bm was detected in 50.87% and Tg in 64.91% of all examined ticks. Co-existence of these both protozoans in 36.84% of total examined ticks was noted. CONCLUSIONS: The study showed a high risk of exposure to Bm and Tg in the studied area. Ticks Ir play an important role in the transmission of Bm in this region. Demonstrating a high percentage of ticks collected from animals infected with Tg may indicate their important role in the transmission of this pathogen, but it requires a further studies.
INTRODUCTION: Ticks (Acari: Ixodida) are vectors of many pathogens i. a: Babesia microti [Bm] and Toxoplasma gondii [Tg]. In Poland, Ixodes ricinus [Ir] is the main vector of both pathogens. This tick species and pathogens transmitted by them are a significant threat to human and animal health. OBJECTIVES OF THE STUDY: Detection of the protozoans Bm and Tg in ticks collected in the Tarnogórski district area. MATERIAL AND METHODS: The ticks were collected from vegetation and pets in the spring period of their activity on the territory of the Tarnogórski district. The parasites were preserved in 70% ethanol. DNA was isolated by ammonia method. Bm was detected by nested-PCR using specific primers for the 18S rRNA sequence. To detect the Tg a commercial kit was used. The PCR products were separated on 2% ethidium bromide stained agarose gels and visualised under UV light. RESULTS: It was showed that all collected ticks belonged to the species Ir. Bm was detected in 50.87% and Tg in 64.91% of all examined ticks. Co-existence of these both protozoans in 36.84% of total examined ticks was noted. CONCLUSIONS: The study showed a high risk of exposure to Bm and Tg in the studied area. Ticks Ir play an important role in the transmission of Bm in this region. Demonstrating a high percentage of ticks collected from animals infected with Tg may indicate their important role in the transmission of this pathogen, but it requires a further studies.
Authors: Piotr Cuber; Aleksandra Urbanek; Aleksandra Naczk; Piotr Stepnowski; Marek Gołębiowski Journal: Exp Appl Acarol Date: 2016-03-14 Impact factor: 2.132
Authors: Samuel Cronhjort; Peter Wilhelmsson; Linda Karlsson; Johanna Thelaus; Andreas Sjödin; Pia Forsberg; Per-Eric Lindgren Journal: Infect Ecol Epidemiol Date: 2019-11-02
Authors: Tal Azagi; Ryanne I Jaarsma; Arieke Docters van Leeuwen; Manoj Fonville; Miriam Maas; Frits F J Franssen; Marja Kik; Jolianne M Rijks; Margriet G Montizaan; Margit Groenevelt; Mark Hoyer; Helen J Esser; Aleksandra I Krawczyk; David Modrý; Hein Sprong; Samiye Demir Journal: Pathogens Date: 2021-03-24