| Literature DB >> 25778491 |
Weijun Liu1, Zhenyong Zhang, Yongxue Zhang, Xinju Chen, Shikui Guo, Yi Lei, Yu Xu, Chao Ji, Zhigang Bi, Kunhua Wang.
Abstract
In the present study, we examined the mechanisms of oxaliplatin-induced drug resistance in human colorectal cancer cell lines HT29 and HCT116. Our results demonstrate a significant autophagy expression in CRC cells after an oxaliplatin treatment. Administration of oxaliplatin to human CRC cells significantly enhanced the expression of HMGB1, which regulated the autophagy response and negatively regulate the cell apoptosis. Moreover, a decreased oxaliplatin -induced autophagy response and an increased apoptosis level were detected in stable CRC cells harboring HMGB1 shRNA. Then we noted that HMGB1 significantly induced extracellular signal-regulated kinase (ERK)/Extracellular signal-regulated kinase kinase (MEK) phosphorylation. Taken together, these data suggest that HMGB1-mediated autophagy modulates sensitivity of colorectal cancer cells to oxaliplatin via MEK/ERK signaling pathway.Entities:
Keywords: 3-MA, 3-methyladenosine; 5-FU, 5-fluorouracil; CCK8, Cell Counting Kit-8; CRC, Colorectal cancer; ERK, extracellular signal-regulated kinase; HMGB1; HMGB1, High–mobility group box 1 protein; MEK, extracellular signal-regulated kinase kinase; MEK/ERK pathway; apoptosis; autophagy; colorectal cancer; oxaliplatin; shRNA, short hairpin RNA
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Year: 2015 PMID： 25778491 PMCID： PMC4622507 DOI： 10.1080/15384047.2015.1017691
Source DB: PubMed Journal: Cancer Biol Ther ISSN： 1538-4047 Impact factor: 4.742