Literature DB >> 25773356

Exploring the protein stability landscape: Bacillus subtilis lipase A as a model for detergent tolerance.

Alexander Fulton1, Victorine Josiane Frauenkron-Machedjou, Pia Skoczinski, Susanne Wilhelm, Leilei Zhu, Ulrich Schwaneberg, Karl-Erich Jaeger.   

Abstract

A systematic study was conducted with Bacillus subtilis lipase A (BSLA) to determine the effect of every single amino acid substitution on detergent tolerance. BSLA is a minimal α/β-hydrolase of 181 amino acids with a known crystal structure. It can be expressed in Escherichia coli and is biochemically well characterized. Site saturation mutagenesis resulted in a library of 3439 variants, each with a single amino acid exchange as confirmed by DNA sequencing. The library was tested against four detergents, namely SDS, CTAB, Tween 80, and sulfobetaine. Surface remodeling emerged as an effective engineering strategy to increase tolerance towards detergents. Amino acid residues that significantly affect the tolerance for each of the four detergents were identified. In summary, this systematic analysis provides an experimental dataset to help derive novel protein engineering strategies as well as to direct modeling efforts.
© 2015 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.

Entities:  

Keywords:  Bacillus subtilis lipase A; alpha beta hydrolases; detergent effects; directed evolution; noncovalent interactions

Mesh：

Substances：

Year:  2015        PMID： 25773356     DOI： 10.1002/cbic.201402664

Source DB:  PubMed          Journal:  Chembiochem        ISSN： 1439-4227            Impact factor:   3.164


  10 in total

1.  Enzymatic removal of protein fouling from self-assembled cellulosic nanofilms: experimental and modeling studies.

Authors:  Sagheer A Onaizi
Journal:  Eur Biophys J       Date:  2018-07-09       Impact factor: 1.733

2.  Contribution of single amino acid and codon substitutions to the production and secretion of a lipase by Bacillus subtilis.

Authors:  Pia Skoczinski; Kristina Volkenborn; Alexander Fulton; Anuseema Bhadauriya; Christina Nutschel; Holger Gohlke; Andreas Knapp; Karl-Erich Jaeger
Journal:  Microb Cell Fact       Date:  2017-09-25       Impact factor: 5.328

3.  A combination of mutational and computational scanning guides the design of an artificial ligand-binding controlled lipase.

Authors:  Marco Kaschner; Oliver Schillinger; Timo Fettweiss; Christina Nutschel; Frank Krause; Alexander Fulton; Birgit Strodel; Andreas Stadler; Karl-Erich Jaeger; Ulrich Krauss
Journal:  Sci Rep       Date:  2017-02-20       Impact factor: 4.379

4.  Can constraint network analysis guide the identification phase of KnowVolution? A case study on improved thermostability of an endo-β-glucanase.

Authors:  Francisca Contreras; Christina Nutschel; Laura Beust; Mehdi D Davari; Holger Gohlke; Ulrich Schwaneberg
Journal:  Comput Struct Biotechnol J       Date:  2020-12-28       Impact factor: 7.271

5.  Critical assessment of structure-based approaches to improve protein resistance in aqueous ionic liquids by enzyme-wide saturation mutagenesis.

Authors:  Till El Harrar; Mehdi D Davari; Karl-Erich Jaeger; Ulrich Schwaneberg; Holger Gohlke
Journal:  Comput Struct Biotechnol J       Date:  2021-12-16       Impact factor: 7.271

6.  Application of Rigidity Theory to the Thermostabilization of Lipase A from Bacillus subtilis.

Authors:  Prakash Chandra Rathi; Alexander Fulton; Karl-Erich Jaeger; Holger Gohlke
Journal:  PLoS Comput Biol       Date:  2016-03-22       Impact factor: 4.475

Review 7.  The Generation and Exploitation of Protein Mutability Landscapes for Enzyme Engineering.

Authors:  Jan-Ytzen van der Meer; Lieuwe Biewenga; Gerrit J Poelarends
Journal:  Chembiochem       Date:  2016-08-19       Impact factor: 3.164

8.  Tuning Enzyme Activity for Nonaqueous Solvents: Engineering an Enantioselective "Michaelase" for Catalysis in High Concentrations of Ethanol.

Authors:  Chao Guo; Lieuwe Biewenga; Max Lubberink; Ronald van Merkerk; Gerrit J Poelarends
Journal:  Chembiochem       Date:  2020-02-18       Impact factor: 3.164

9.  Deletion and Randomization of Structurally Variable Regions in B. subtilis Lipase A (BSLA) Alter Its Stability and Hydrolytic Performance Against Long Chain Fatty Acid Esters.

Authors:  Ronny Martínez; Claudia Bernal; Rodrigo Álvarez; Christopher Concha; Fernando Araya; Ricardo Cabrera; Gaurao V Dhoke; Mehdi D Davari
Journal:  Int J Mol Sci       Date:  2020-03-14       Impact factor: 5.923

Review 10.  High-throughput screening, next generation sequencing and machine learning: advanced methods in enzyme engineering.

Authors:  Rosario Vanella; Gordana Kovacevic; Vanni Doffini; Jaime Fernández de Santaella; Michael A Nash
Journal:  Chem Commun (Camb)       Date:  2022-02-17       Impact factor: 6.222
  10 in total

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