Literature DB >> 25762419

Whole-Exome Enrichment with the Roche NimbleGen SeqCap EZ Exome Library SR Platform.

Rui Chen1, Hogune Im1, Michael Snyder1.   

Abstract

Multiple platforms are available for whole-exome enrichment and sequencing (WES). This protocol is based on the Roche NimbleGen SeqCap EZ Exome Library SR platform, which enriches for ∼44 Mb of the human exonic regions. The SeqCap system uses 55- to 105-base DNA probes to capture known coding DNA sequences (CDS) from the NCBI Consensus CDS Database, RefSeq, and Sanger miRBase. The protocol can be performed at the benchside without the need for automation, and the resulting library can be used for targeted next-generation sequencing on an Illumina HiSeq 2000 sequencer.
© 2015 Cold Spring Harbor Laboratory Press.

Entities:  

Mesh：

Year:  2015        PMID： 25762419      PMCID： PMC4490009          DOI： 10.1101/pdb.prot084855

Source DB:  PubMed          Journal:  Cold Spring Harb Protoc        ISSN： 1559-6095


  3 in total

1.  Whole-Exome Enrichment with the Illumina TruSeq Exome Enrichment Platform.

Authors:  Rui Chen; Hogune Im; Michael Snyder
Journal:  Cold Spring Harb Protoc       Date:  2015-03-11

2.  Whole-Exome Enrichment with the Agilent SureSelect Human All Exon Platform.

Authors:  Rui Chen; Hogune Im; Michael Snyder
Journal:  Cold Spring Harb Protoc       Date:  2015-03-11

3.  Performance comparison of exome DNA sequencing technologies.

Authors:  Michael J Clark; Rui Chen; Hugo Y K Lam; Konrad J Karczewski; Rong Chen; Ghia Euskirchen; Atul J Butte; Michael Snyder
Journal:  Nat Biotechnol       Date:  2011-09-25       Impact factor: 68.164
  3 in total
  7 in total

1.  A new POT1 germline mutation-expanding the spectrum of POT1-associated cancers.

Authors:  Tremika Le-Shan Wilson; Namita Hattangady; Antonio Marcondes Lerario; Carmen Williams; Erika Koeppe; Shane Quinonez; Jenae Osborne; Kelly B Cha; Tobias Else
Journal:  Fam Cancer       Date:  2017-10       Impact factor: 2.375

2.  Patient-Derived Triple-Negative Breast Cancer Organoids Provide Robust Model Systems That Recapitulate Tumor Intrinsic Characteristics.

Authors:  Melissa Kramer; Suzanne Russo; Payal Naik; Sonam Bhatia; Gayatri Arun; Kyle Brophy; Peter Andrews; Cheng Fan; Charles M Perou; Jonathan Preall; Taehoon Ha; Dennis Plenker; David A Tuveson; Arvind Rishi; John E Wilkinson; W Richard McCombie; Karen Kostroff; David L Spector
Journal:  Cancer Res       Date:  2022-04-01       Impact factor: 13.312

3.  Whole-Exome Enrichment with the Illumina TruSeq Exome Enrichment Platform.

Authors:  Rui Chen; Hogune Im; Michael Snyder
Journal:  Cold Spring Harb Protoc       Date:  2015-03-11

4.  Whole-Exome Enrichment with the Agilent SureSelect Human All Exon Platform.

Authors:  Rui Chen; Hogune Im; Michael Snyder
Journal:  Cold Spring Harb Protoc       Date:  2015-03-11

5.  LIFE-Seq: a universal Large Integrated DNA Fragment Enrichment Sequencing strategy for deciphering the transgene integration of genetically modified organisms.

Authors:  Hanwen Zhang; Rong Li; Yongkun Guo; Yuchen Zhang; Dabing Zhang; Litao Yang
Journal:  Plant Biotechnol J       Date:  2022-02-01       Impact factor: 13.263

6.  A genetic risk factor for thrombophilia in a Han Chinese family.

Authors:  Guoping Sun; Yicong Jia; Jingye Meng; Minglin Ou; Peng Zhu; Shan Cong; Yadan Luo; Weiguo Sui; Yong Dai
Journal:  Mol Med Rep       Date:  2017-02-17       Impact factor: 2.952

7.  Novel variants in the KERA gene cause autosomal recessive cornea plana in a Chinese family: A case report.

Authors:  Chengzi Huang; Xigui Long; Can Peng; Pengsiyuan Lin; Hu Tan; Weigang Lv; Lingqian Wu
Journal:  Mol Med Rep       Date:  2019-04-11       Impact factor: 2.952
  7 in total

北京卡尤迪生物科技股份有限公司 © 2022-2023.