Literature DB >> 25760708

Crystallization and preliminary crystallographic analysis of acetophenone reductase from Geotrichum candidum NBRC 4597.

Yosuke Sugiyama1, Miki Senda2, Toshiya Senda2, Tomoko Matsuda1.   

Abstract

Acetophenone reductase (APRD) from Geotrichum candidium NBRC 4597 was crystallized by the hanging-drop vapour-diffusion method using PEG 3350 as a precipitant. The crystal belonged to space group P6522, with unit-cell parameters a = b = 104.5, c = 273.7 Å, and diffracted to 2.6 Å resolution. Phasing using the single-wavelength anomalous diffraction method was successful. Model building and crystallographic refinement are in progress.

Entities:  

Keywords:  acetophenone reductase; asymmetric reduction; enantioselectivity

Mesh：

Substances：

Year:  2015        PMID： 25760708      PMCID： PMC4356309          DOI： 10.1107/S2053230X15002265

Source DB:  PubMed          Journal:  Acta Crystallogr F Struct Biol Commun        ISSN： 2053-230X            Impact factor:   1.056


  12 in total

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