Literature DB >> 24834985

Unconserved substrate-binding sites direct the stereoselectivity of medium-chain alcohol dehydrogenase.

Shanshan Wang1, Yao Nie, Yan Xu, Rongzhen Zhang, Tzu-Ping Ko, Chun-Hsiang Huang, Hsiu-Chien Chan, Rey-Ting Guo, Rong Xiao.   

Abstract

Structure-guided design of substrate-binding pocket inversed the stereoselectivity of an NADH-dependent medium-chain alcohol dehydrogenase (MDR) from Prelog to anti-Prelog. The pocket-forming amino acids, especially the unconserved residues as hotspots, play critical roles in directing MDRs' stereoselectivity.

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Year:  2014        PMID： 24834985     DOI： 10.1039/c4cc01752h

Source DB:  PubMed          Journal:  Chem Commun (Camb)        ISSN： 1359-7345            Impact factor:   6.222


  5 in total

1.  Crystallization and preliminary crystallographic analysis of acetophenone reductase from Geotrichum candidum NBRC 4597.

Authors:  Yosuke Sugiyama; Miki Senda; Toshiya Senda; Tomoko Matsuda
Journal:  Acta Crystallogr F Struct Biol Commun       Date:  2015-02-19       Impact factor: 1.056

2.  Activity prediction of substrates in NADH-dependent carbonyl reductase by docking requires catalytic constraints and charge parameterization of catalytic zinc environment.

Authors:  Gaurao V Dhoke; Christoph Loderer; Mehdi D Davari; Marion Ansorge-Schumacher; Ulrich Schwaneberg; Marco Bocola
Journal:  J Comput Aided Mol Des       Date:  2015-11-03       Impact factor: 3.686

Review 3.  Alcohol Dehydrogenases with anti-Prelog Stereopreference in Synthesis of Enantiopure Alcohols.

Authors:  Musa M Musa
Journal:  ChemistryOpen       Date:  2022-02-22       Impact factor: 2.630

Review 4.  Redesigning Enzymes for Biocatalysis: Exploiting Structural Understanding for Improved Selectivity.

Authors:  Yaoyu Ding; Gustavo Perez-Ortiz; Jessica Peate; Sarah M Barry
Journal:  Front Mol Biosci       Date:  2022-07-22

5.  Reversal of Regioselectivity in Zinc-Dependent Medium-Chain Alcohol Dehydrogenase from Rhodococcus erythropolis toward Octanone Derivatives.

Authors:  Gaurao V Dhoke; Yunus Ensari; Dinc Yasat Hacibaloglu; Anna Gärtner; Anna Joëlle Ruff; Marco Bocola; Mehdi D Davari
Journal:  Chembiochem       Date:  2020-06-30       Impact factor: 3.164
  5 in total

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