AIM: To investigate differences in microbes and short chain fatty acid (SCFA) levels in stool samples from Hispanic and non-Hispanic African American, American Indian, and White participants. METHODS: Stool samples from twenty participants were subjected to analysis for relative levels of viable bacteria and for SCFA levels. Additionally, the samples were subjected to 16S rRNA gene pyrosequencing for identification of bacteria present in the stool. We used a metagenome functional prediction technique to analyze genome copy numbers and estimate the abundance of butyrate kinase in all samples. RESULTS: We found that African Americans had significantly lower levels of acetate, butyrate, and total SCFAs than all other racial/ethnic groups. We also found that participant microbial profiles differed by racial/ethnic group. African Americans had significantly more Firmicutes than Whites, with enriched Ruminococcaceae. The Firmicutes/Bacteroidetes ratio was also significantly higher for African Americans than for Whites (P = 0.049). We found Clostridium levels to be significantly and inversely related to total SCFA levels (P = 0.019) and we found Bacteroides to be positively associated (P = 0.027) and Clostridium to be negatively associated (P = 0.012) with levels of butyrate. We also identified a correlation between copy number for a butyrate kinase predicted from 16S rRNA gene abundance and levels of butyrate in stool. CONCLUSION: The identified differences in gut flora and SCFA levels may relate to colorectal cancer mortality differentials and may be useful as targets for future clinical and behavioral interventions.
AIM: To investigate differences in microbes and short chain fatty acid (SCFA) levels in stool samples from Hispanic and non-Hispanic African American, American Indian, and White participants. METHODS: Stool samples from twenty participants were subjected to analysis for relative levels of viable bacteria and for SCFA levels. Additionally, the samples were subjected to 16S rRNA gene pyrosequencing for identification of bacteria present in the stool. We used a metagenome functional prediction technique to analyze genome copy numbers and estimate the abundance of butyrate kinase in all samples. RESULTS: We found that African Americans had significantly lower levels of acetate, butyrate, and total SCFAs than all other racial/ethnic groups. We also found that participant microbial profiles differed by racial/ethnic group. African Americans had significantly more Firmicutes than Whites, with enriched Ruminococcaceae. The Firmicutes/Bacteroidetes ratio was also significantly higher for African Americans than for Whites (P = 0.049). We found Clostridium levels to be significantly and inversely related to total SCFA levels (P = 0.019) and we found Bacteroides to be positively associated (P = 0.027) and Clostridium to be negatively associated (P = 0.012) with levels of butyrate. We also identified a correlation between copy number for a butyrate kinase predicted from 16S rRNA gene abundance and levels of butyrate in stool. CONCLUSION: The identified differences in gut flora and SCFA levels may relate to colorectal cancer mortality differentials and may be useful as targets for future clinical and behavioral interventions.
Authors: J S Mandel; T R Church; J H Bond; F Ederer; M S Geisser; S J Mongin; D C Snover; L M Schuman Journal: N Engl J Med Date: 2000-11-30 Impact factor: 91.245
Authors: Greg Yothers; Daniel J Sargent; Norman Wolmark; Richard M Goldberg; Michael J O'Connell; Jacqueline K Benedetti; Leonard B Saltz; James J Dignam; A William Blackstock Journal: J Natl Cancer Inst Date: 2011-10-12 Impact factor: 13.506
Authors: Julian R Marchesi; Bas E Dutilh; Neil Hall; Wilbert H M Peters; Rian Roelofs; Annemarie Boleij; Harold Tjalsma Journal: PLoS One Date: 2011-05-24 Impact factor: 3.240
Authors: Han Shuwen; Da Miao; Qi Quan; Wu Wei; Zhang Zhongshan; Zhang Chun; Yang Xi Journal: J Cancer Res Clin Oncol Date: 2019-08-10 Impact factor: 4.553
Authors: Naomi L Ward; Caleb D Phillips; Deanna D Nguyen; Nanda Kumar N Shanmugam; Yan Song; Richard Hodin; Hai Ning Shi; Bobby J Cherayil; Allan M Goldstein Journal: Inflamm Bowel Dis Date: 2016-10 Impact factor: 5.325
Authors: Velda J González-Mercado; Jean Lim; Lawrence Berk; Mary Esele; Carmen S Rodríguez; Gerardo Colón-Otero Journal: Curr Probl Cancer Date: 2020-01-27 Impact factor: 3.187
Authors: Tiffany L Carson; Fuchenchu Wang; Xiangqin Cui; Bradford E Jackson; William J Van Der Pol; Elliot J Lefkowitz; Casey Morrow; Monica L Baskin Journal: Psychosom Med Date: 2018-09 Impact factor: 4.312
Authors: Fernando F Anhê; Thibault V Varin; Mélanie Le Barz; Yves Desjardins; Emile Levy; Denis Roy; André Marette Journal: Curr Obes Rep Date: 2015-12