Ann R Strom1, Dennis E Cortés2,3, Sara M Thomasy1, Philip H Kass4, Mark J Mannis2, Christopher J Murphy1,2. 1. Department of Veterinary Surgical and Radiological Sciences, School of Veterinary Medicine, University of California, Davis, CA, 95616, USA. 2. Department of Ophthalmology & Vision Science, UC Davis Eye Center, School of Medicine, University of California, Davis, CA, 95616, USA. 3. Department of Ophthalmology, Pontificia Universidad Católica de Chile, Santiago, Chile. 4. Department of Population Health and Reproduction, School of Veterinary Medicine and School of Medicine, University of California, Davis, CA, 95616, USA.
Abstract
OBJECTIVE: To obtain normative data for the normal laboratory beagle cornea using high-resolution in vivo confocal microscopy (IVCM). ANIMALS STUDIED: Sixteen eyes of eight healthy young female intact beagles. PROCEDURES: The central cornea was imaged using IVCM. Mixed effects linear regression was used for statistical analysis. RESULTS: in vivo confocal microscopy allowed detailed visualization and quantification of epithelial cells (superficial epithelial cell diameter: 43.25 ± 6.64 μm, basal cell diameter: 4.43 ± 0.67 μm), and nerve fibers (subepithelial nerve fiber diameter: 2.38 ± 0.69 μm, anterior stromal nerve fiber diameter: 16.93 ± 4.55 μm). Keratocyte density (anterior stroma 993.38 ± 134.24 cells/mm(2) , posterior stroma 789.38 ± 87.13 cells/mm(2) ) and endothelial cell density (2815.18 ± 212.59 cells/mm(2) ) were also measured. CONCLUSION: High-resolution IVCM provides detailed noninvasive evaluation of the cornea in the normal laboratory beagle.
OBJECTIVE: To obtain normative data for the normal laboratory beagle cornea using high-resolution in vivo confocal microscopy (IVCM). ANIMALS STUDIED: Sixteen eyes of eight healthy young female intact beagles. PROCEDURES: The central cornea was imaged using IVCM. Mixed effects linear regression was used for statistical analysis. RESULTS: in vivo confocal microscopy allowed detailed visualization and quantification of epithelial cells (superficial epithelial cell diameter: 43.25 ± 6.64 μm, basal cell diameter: 4.43 ± 0.67 μm), and nerve fibers (subepithelial nerve fiber diameter: 2.38 ± 0.69 μm, anterior stromal nerve fiber diameter: 16.93 ± 4.55 μm). Keratocyte density (anterior stroma 993.38 ± 134.24 cells/mm(2) , posterior stroma 789.38 ± 87.13 cells/mm(2) ) and endothelial cell density (2815.18 ± 212.59 cells/mm(2) ) were also measured. CONCLUSION: High-resolution IVCM provides detailed noninvasive evaluation of the cornea in the normal laboratory beagle.
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