Ming-Kun Diao1, Chu-Yi Liu2, Hong-Wei Liu1, Jin-Tao Li3, Fan Li2, Mohammadreza Mohammadzad Mehryar2, Yang-Junqi Wang2, Shao-Bing Zhan4, Yu-Bai Zhou2, Ru-Gang Zhong2, Yi Zeng5. 1. National Institute for Viral Disease Control and Prevention, Chinese Center for Disease Control and Prevention, and State Key Laboratory for Infectious Disease Prevention and Control, Beijing 100052, China; Beijing Key Laboratory of Environmental and Viral Oncology, College of Life Science and Bio-Engineering, Beijing University of Technology, Beijing 100124, China. 2. Beijing Key Laboratory of Environmental and Viral Oncology, College of Life Science and Bio-Engineering, Beijing University of Technology, Beijing 100124, China. 3. National Institute for Viral Disease Control and Prevention, Chinese Center for Disease Control and Prevention, and State Key Laboratory for Infectious Disease Prevention and Control, Beijing 100052, China; Beijing Key Laboratory of Environmental and Viral Oncology, College of Life Science and Bio-Engineering, Beijing University of Technology, Beijing 100124, China. Electronic address: ljt2000593@163.com. 4. National Institute for Viral Disease Control and Prevention, Chinese Center for Disease Control and Prevention, and State Key Laboratory for Infectious Disease Prevention and Control, Beijing 100052, China. 5. National Institute for Viral Disease Control and Prevention, Chinese Center for Disease Control and Prevention, and State Key Laboratory for Infectious Disease Prevention and Control, Beijing 100052, China; Beijing Key Laboratory of Environmental and Viral Oncology, College of Life Science and Bio-Engineering, Beijing University of Technology, Beijing 100124, China. Electronic address: zengyicdc1@gmail.com.
Abstract
AIMS: The integration preferences of human papillomavirus (HPV) have been intensively studied and contested over recent years. To disclose the integration preferences of high-risk HPV in cervical cancer, HPV transcriptional sites and features in different cervical cancer cell lines were identified. MAIN METHODS: In this study, three cervical cancer cell lines (CaSki, HeLa, and SiHa) were subjected for HPV genome status determination by amplification of papillomavirus oncogene transcripts (APOT) assay. The numbers of viral copies in human genomes and numbers of viral-human fusion mRNAs in three HPV-integrated cervical cancer cell lines were measured and analysed. KEY FINDINGS: The results revealed that the gene desert region 8q24 of the HPV type 18 integrated HeLa cell line and the 13q21-22 region of the HPV type 16 integrated CaSki and SiHa cell lines were hotspots for HPV integration, and the numbers of viral copies in the human genomes of the three cell lines that we detected were not in accordance with those reported in previous studies. SIGNIFICANCE: Integration of the HPV genome into the host cell chromosome suggests that persistent HPV infection is vital for malignant cell transformation and carcinogenesis. This study provides information to benefit health care professionals seeking more comprehensive and accurate diagnostics for HPV-related disease"? Please check, and amend as necessary.
AIMS: The integration preferences of human papillomavirus (HPV) have been intensively studied and contested over recent years. To disclose the integration preferences of high-risk HPV in cervical cancer, HPV transcriptional sites and features in different cervical cancer cell lines were identified. MAIN METHODS: In this study, three cervical cancer cell lines (CaSki, HeLa, and SiHa) were subjected for HPV genome status determination by amplification of papillomavirus oncogene transcripts (APOT) assay. The numbers of viral copies in human genomes and numbers of viral-human fusion mRNAs in three HPV-integrated cervical cancer cell lines were measured and analysed. KEY FINDINGS: The results revealed that the gene desert region 8q24 of the HPV type 18 integrated HeLa cell line and the 13q21-22 region of the HPV type 16 integrated CaSki and SiHa cell lines were hotspots for HPV integration, and the numbers of viral copies in the human genomes of the three cell lines that we detected were not in accordance with those reported in previous studies. SIGNIFICANCE: Integration of the HPV genome into the host cell chromosome suggests that persistent HPV infection is vital for malignant cell transformation and carcinogenesis. This study provides information to benefit health care professionals seeking more comprehensive and accurate diagnostics for HPV-related disease"? Please check, and amend as necessary.
Authors: John Charles Rotondo; Lucia Oton-Gonzalez; Chiara Mazziotta; Carmen Lanzillotti; Maria Rosa Iaquinta; Mauro Tognon; Fernanda Martini Journal: Front Microbiol Date: 2020-11-19 Impact factor: 5.640
Authors: Sen Ding; Steven Y Qian; Yang Zhang; Wenlei Wu; Gensheng Lu; Yan Lu; Xiujing Feng; Li Li; Pingping Shen Journal: Sci Rep Date: 2015-09-03 Impact factor: 4.379
Authors: Maria Pujantell; Roger Badia; Iván Galván-Femenía; Edurne Garcia-Vidal; Rafael de Cid; Carmen Alcalde; Antonio Tarrats; Marta Piñol; Francesc Garcia; Ana M Chamorro; Boris Revollo; Sebastian Videla; David Parés; Javier Corral; Cristina Tural; Guillem Sirera; José A Esté; Ester Ballana; Eva Riveira-Muñoz Journal: Sci Rep Date: 2019-12-27 Impact factor: 4.379