Literature DB >> 2573822

Analysis of expression of hybrid yeast genes containing ARS elements.

D Kipling1, S E Kearsey.   

Abstract

In an attempt to devise a new assay for ARS-binding proteins we have inserted the HO ARS between the upstream activation site and the TATA region of the yeast CYC1 promoter. A marked reduction in promoter activity is observed. Inactivation of the HO ARS element by point mutation does not restore promoter activity to its original level, although a modest activation is seen. We have also inserted the HO ARS into the intron of the yeast actin gene; although there is no apparent deleterious effect on transcription, the activity of the ARS is abolished in this new environment.

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Year:  1989        PMID： 2573822     DOI： 10.1007/BF00332420

Source DB:  PubMed          Journal:  Mol Gen Genet        ISSN： 0026-8925


  23 in total

1.  Cloning of large segments of exogenous DNA into yeast by means of artificial chromosome vectors.

Authors:  D T Burke; G F Carle; M V Olson
Journal:  Science       Date:  1987-05-15       Impact factor: 47.728

2.  Chromosomal ARS and CEN elements bind specifically to the yeast nuclear scaffold.

Authors:  B B Amati; S M Gasser
Journal:  Cell       Date:  1988-09-23       Impact factor: 41.582

Review 3.  Yeast chromosome replication and segregation.

Authors:  C S Newlon
Journal:  Microbiol Rev       Date:  1988-12

4.  Negative control of oriC plasmid replication by transcription of the oriC region.

Authors:  M Tanaka; S Hiraga
Journal:  Mol Gen Genet       Date:  1985

5.  Molecular analysis of a cell lineage.

Authors:  K Nasmyth
Journal:  Nature       Date:  1983-04-21       Impact factor: 49.962

6.  A bacterial repressor protein or a yeast transcriptional terminator can block upstream activation of a yeast gene.

Authors:  R Brent; M Ptashne
Journal:  Nature       Date:  1984 Dec 13-19       Impact factor: 49.962

7.  Activation of a cryptic TACTAAC box in the Saccharomyces cerevisiae actin intron.

Authors:  A Cellini; R Parker; J McMahon; C Guthrie; J Rossi
Journal:  Mol Cell Biol       Date:  1986-05       Impact factor: 4.272

8.  Plasmids pEMBLY: new single-stranded shuttle vectors for the recovery and analysis of yeast DNA sequences.

Authors:  C Baldari; G Cesareni
Journal:  Gene       Date:  1985       Impact factor: 3.688

9.  Size and position of intervening sequences are critical for the splicing efficiency of pre-mRNA in the yeast Saccharomyces cerevisiae.

Authors:  F J Klinz; D Gallwitz
Journal:  Nucleic Acids Res       Date:  1985-06-11       Impact factor: 16.971

10.  Transcription interferes with elements important for chromosome maintenance in Saccharomyces cerevisiae.

Authors:  M Snyder; R J Sapolsky; R W Davis
Journal:  Mol Cell Biol       Date:  1988-05       Impact factor: 4.272
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  4 in total

1.  Identification of an origin of bidirectional DNA replication in the ubiquitously expressed mammalian CAD gene.

Authors:  R E Kelly; M L DeRose; B W Draper; G M Wahl
Journal:  Mol Cell Biol       Date:  1995-08       Impact factor: 4.272

2.  Transcription through the yeast origin of replication ARS1 ends at the ABFI binding site and affects extrachromosomal maintenance of minichromosomes.

Authors:  S Tanaka; D Halter; M Livingstone-Zatchej; B Reszel; F Thoma
Journal:  Nucleic Acids Res       Date:  1994-09-25       Impact factor: 16.971

3.  Transcription inhibits the replication of autonomously replicating plasmids in human cells.

Authors:  S B Haase; S S Heinzel; M P Calos
Journal:  Mol Cell Biol       Date:  1994-04       Impact factor: 4.272

4.  rar mutations which increase artificial chromosome stability in Saccharomyces cerevisiae identify transcription and recombination proteins.

Authors:  D Kipling; C Tambini; S E Kearsey
Journal:  Nucleic Acids Res       Date:  1991-04-11       Impact factor: 16.971
  4 in total

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