BACKGROUND: Antimicrobial photodynamic inactivation with fullerenes bearing cationic charges may overcome resistant microbes. METHODS & RESULTS: We synthesized C60-fullerene (LC16) bearing decaquaternary chain and deca-tertiary-amino groups that facilitates electron-transfer reactions via the photoexcited fullerene. Addition of the harmless salt, potassium iodide (10 mM) potentiated the ultraviolet A (UVA) or white light-mediated killing of Gram-negative bacteria Acinetobacter baumannii, Gram-positive methicillin-resistant Staphylococcus aureus and fungal yeast Candida albicans by 1-2+ logs. Mouse model infected with bioluminescent Acinetobacter baumannii gave increased loss of bioluminescence when iodide (10 mM) was combined with LC16 and UVA/white light. CONCLUSION: The mechanism may involve photoinduced electron reduction of (1)(C60>)* or (3)(C60>)* by iodide producing I· or I2 followed by subsequent intermolecular electron-transfer events of (C60>)-· to produce reactive radicals.
BACKGROUND: Antimicrobial photodynamic inactivation with fullerenes bearing cationic charges may overcome resistant microbes. METHODS & RESULTS: We synthesized C60-fullerene (LC16) bearing decaquaternary chain and deca-tertiary-amino groups that facilitates electron-transfer reactions via the photoexcited fullerene. Addition of the harmless salt, potassium iodide (10 mM) potentiated the ultraviolet A (UVA) or white light-mediated killing of Gram-negative bacteria Acinetobacter baumannii, Gram-positive methicillin-resistant Staphylococcus aureus and fungal yeastCandida albicans by 1-2+ logs. Mouse model infected with bioluminescent Acinetobacter baumannii gave increased loss of bioluminescence when iodide (10 mM) was combined with LC16 and UVA/white light. CONCLUSION: The mechanism may involve photoinduced electron reduction of (1)(C60>)* or (3)(C60>)* by iodide producing I· or I2 followed by subsequent intermolecular electron-transfer events of (C60>)-· to produce reactive radicals.
Entities:
Keywords:
antimicrobial photodynamic therapy; bioluminescent bacteria; decacationic fullerene; decatertiary-amine chain; in vivo infection model; iodide potentiation
Authors: A Minnock; D I Vernon; J Schofield; J Griffiths; J H Parish; S B Brown Journal: Antimicrob Agents Chemother Date: 2000-03 Impact factor: 5.191
Authors: Domingo Mariano Adolfo Vera; Mark H Haynes; Anthony R Ball; Tianhong Dai; Christos Astrakas; Michael J Kelso; Michael R Hamblin; George P Tegos Journal: Photochem Photobiol Date: 2012-02-13 Impact factor: 3.421
Authors: Xavier Ragàs; Tianhong Dai; George P Tegos; Montserrat Agut; Santi Nonell; Michael R Hamblin Journal: Lasers Surg Med Date: 2010-07 Impact factor: 4.025
Authors: Felipe F Sperandio; Sulbha K Sharma; Min Wang; Seaho Jeon; Ying-Ying Huang; Tianhong Dai; Suhasini Nayka; Suzana C O M de Sousa; Long Y Chiang; Michael R Hamblin Journal: Nanomedicine Date: 2012-10-29 Impact factor: 5.307
Authors: Maximiliano L Agazzi; Javier E Durantini; Ezequiel D Quiroga; M Gabriela Alvarez; Edgardo N Durantini Journal: Photochem Photobiol Sci Date: 2021-03-04 Impact factor: 3.982