Javier A Jo1, Jesung Park2, Paritosh Pande2, Sebina Shrestha2, Michael J Serafino2, J de Jesus Rico Jimenez2, Fred Clubb3, Brian Walton4, L Maximilian Buja5, Jennifer E Phipps6, Marc D Feldman6, Jessie Adame7, Brian E Applegate2. 1. Department of Biomedical Engineering, Texas A&M University, 5062 Emerging Technologies Building, 3120 TAMU, College Station, TX 77843-3120, USA javierjo@tamu.edu. 2. Department of Biomedical Engineering, Texas A&M University, 5062 Emerging Technologies Building, 3120 TAMU, College Station, TX 77843-3120, USA. 3. Department of Biomedical Engineering, Texas A&M University, 5062 Emerging Technologies Building, 3120 TAMU, College Station, TX 77843-3120, USA Department of Veterinary Pathobiology, Texas A&M University, College Station, TX, USA. 4. Department of Cardiology, Texas Heart Institute at St. Luke's Episcopal Hospital, Houston, TX, USA. 5. Department of Cardiovascular Pathology Research, Texas Heart Institute at St. Luke's Episcopal Hospital, Houston, TX, USA. 6. University of Texas Health Science Center San Antonio, San Antonio, TX, USA. 7. Autopsy and Pathology Services, Houston, TX, USA.
Abstract
AIMS: The aim of this study was to validate novel imaging technology for simultaneous morphological and biochemical endogenous optical imaging of coronary atherosclerotic plaque. METHODS AND RESULTS: Optical coherence tomography (OCT) generates high-resolution 3D images of plaque morphology and endogenous fluorescence lifetime imaging microscopy (FLIM) characterizes biochemical composition. Both imaging modalities rely on plaque's intrinsic optical characteristics, making contrast agents unnecessary. A multimodal OCT/FLIM system was utilized to generate luminal biochemical maps superimposed on high-resolution (7 µm axial and 13 µm lateral) structural volumetric images. Forty-seven fresh postmortem human coronary segments were imaged: pathological intimal thickening (PIT, n = 26), fibroatheroma (FA, n = 12), thin-cap FA (TCFA, n = 2), and fibrocalcific plaque (CA, n = 7), determined by histopathology. Multimodal images were evaluated, and each plaque identified as PIT, FA, TCFA, or CA based on expert OCT readers, and as having high-lipid (HL), high-collagen (HC), or low-collagen/low-lipid (LCL) luminal composition based on linear discriminant analysis of FLIM. Of 47 plaques, 89.4% (42/47) of the plaques were correctly identified based on OCT/FLIM evaluation using tissue histopathology and immunohistochemistry as the gold standard. Four of the misclassifications corresponded to confusing PIT with HL luminal composition for FA with HL cap. The other corresponded to confusing FA with a HC cap for FA with an LCL cap. CONCLUSION: We have demonstrated the feasibility of accurate simultaneous OCT/FLIM morphological and biochemical characterization of coronary plaques at spatial resolutions and acquisition speeds compatible with catheter-based intravascular imaging. The success of this pilot study sets up future development of a multimodal intravascular imaging system that will enable studies that could help improve our understanding of plaque pathogenesis. Published on behalf of the European Society of Cardiology. All rights reserved.
AIMS: The aim of this study was to validate novel imaging technology for simultaneous morphological and biochemical endogenous optical imaging of coronary atherosclerotic plaque. METHODS AND RESULTS: Optical coherence tomography (OCT) generates high-resolution 3D images of plaque morphology and endogenous fluorescence lifetime imaging microscopy (FLIM) characterizes biochemical composition. Both imaging modalities rely on plaque's intrinsic optical characteristics, making contrast agents unnecessary. A multimodal OCT/FLIM system was utilized to generate luminal biochemical maps superimposed on high-resolution (7 µm axial and 13 µm lateral) structural volumetric images. Forty-seven fresh postmortem human coronary segments were imaged: pathological intimal thickening (PIT, n = 26), fibroatheroma (FA, n = 12), thin-cap FA (TCFA, n = 2), and fibrocalcific plaque (CA, n = 7), determined by histopathology. Multimodal images were evaluated, and each plaque identified as PIT, FA, TCFA, or CA based on expert OCT readers, and as having high-lipid (HL), high-collagen (HC), or low-collagen/low-lipid (LCL) luminal composition based on linear discriminant analysis of FLIM. Of 47 plaques, 89.4% (42/47) of the plaques were correctly identified based on OCT/FLIM evaluation using tissue histopathology and immunohistochemistry as the gold standard. Four of the misclassifications corresponded to confusing PIT with HL luminal composition for FA with HL cap. The other corresponded to confusing FA with a HC cap for FA with an LCL cap. CONCLUSION: We have demonstrated the feasibility of accurate simultaneous OCT/FLIM morphological and biochemical characterization of coronary plaques at spatial resolutions and acquisition speeds compatible with catheter-based intravascular imaging. The success of this pilot study sets up future development of a multimodal intravascular imaging system that will enable studies that could help improve our understanding of plaque pathogenesis. Published on behalf of the European Society of Cardiology. All rights reserved.
Authors: Matthew O'Donnell; Elliot R McVeigh; H William Strauss; Atsushi Tanaka; Brett E Bouma; Guillermo J Tearney; Michael A Guttman; Ernest V Garcia Journal: J Nucl Med Date: 2010-05-01 Impact factor: 10.057
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Authors: David M Small; Jason S Jones; Irwin I Tendler; Paul E Miller; Andre Ghetti; Nozomi Nishimura Journal: Biomed Opt Express Date: 2017-12-13 Impact factor: 3.732
Authors: Jose J Rico-Jimenez; Michael J Serafino; Sebina Shrestha; Xi Chen; Wihan Kim; Jessie Adame; L Maximillan Buja; Deborah Vela; Brian E Applegate; Javier A Jo Journal: Atherosclerosis Date: 2019-04-19 Impact factor: 5.162
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