Jung-Ae Kim1,2, Fatih Karadeniz2, Byul-Nim Ahn3, Myeong Sook Kwon1, Ok-Ju Mun1, Min Joo Bae1, Youngwan Seo4,5, Mihyang Kim1, Sang-Hyeon Lee6, Yuck Yong Kim7, Jang Mi-Soon8, Chang-Suk Kong1. 1. Department of Food and Nutrition, College of Medical and Life Science, Silla University, Busan 617-736, Republic of Korea. 2. Marine Biotechnology Center for Pharmaceuticals and Foods, Silla University, Busan 617-736, Republic of Korea. 3. Department of Organic Material Science and Engineering, Pusan National University, Busan, Republic of Korea. 4. Marine Environment and Bioscience, Korea Maritime University, Busan 606-791, Republic of Korea. 5. Ocean Science & Technology School, Korea Marine University, Busan 606-791, Republic of Korea. 6. Bioscience and Biotechnology Department, Graduate School, Silla University, Busan 617-736, Republic of Korea. 7. IS Food Co., Marine Bio-industry Department Center, Busan 619-912, Republic of Korea. 8. National Fisheries Research & Development Institute, Food and Safety Research Center, Busan 619-705, Republic of Korea.
Abstract
BACKGROUND: Health problems related to the lack of bone formation are a major problem for ageing populations in the modern world. As a part of the ongoing trend to develop natural substances that attenuate bone loss in osteoporosis, the effects of the edible brown alga Sargassum thunbergii and its active contents on adipogenic differentiation in 3T3-L1 fibroblasts and osteoblast differentiation in MC3T3-E1 pre-osteoblasts were evaluated. RESULTS: Treatment with S. thunbergii significantly reduced lipid accumulation and expression of adipogenic differentiation markers such as peroxisome proliferator-activated receptor γ, CCAAT/enhancer-binding protein α and sterol regulatory element binding protein 1c. In addition, S. thunbergii successfully enhanced osteoblast differentiation as indicated by increased alkaline phosphatase activity along raised levels of osteoblastogenesis indicators, namely bone morphogenetic protein-2, osteocalcin and collagen type I. Two compounds, sargaquinoic and sargahydroquinoic acid, were isolated from active extract and shown to be active by means of osteogenesis inducement. CONCLUSION: S. thunbergii could be a source for functional food ingredients for improved treatment of osteoporosis and obesity.
BACKGROUND: Health problems related to the lack of bone formation are a major problem for ageing populations in the modern world. As a part of the ongoing trend to develop natural substances that attenuate bone loss in osteoporosis, the effects of the edible brown alga Sargassum thunbergii and its active contents on adipogenic differentiation in 3T3-L1 fibroblasts and osteoblast differentiation in MC3T3-E1 pre-osteoblasts were evaluated. RESULTS: Treatment with S. thunbergii significantly reduced lipid accumulation and expression of adipogenic differentiation markers such as peroxisome proliferator-activated receptor γ, CCAAT/enhancer-binding protein α and sterol regulatory element binding protein 1c. In addition, S. thunbergii successfully enhanced osteoblast differentiation as indicated by increased alkaline phosphatase activity along raised levels of osteoblastogenesis indicators, namely bone morphogenetic protein-2, osteocalcin and collagen type I. Two compounds, sargaquinoic and sargahydroquinoic acid, were isolated from active extract and shown to be active by means of osteogenesis inducement. CONCLUSION:S. thunbergii could be a source for functional food ingredients for improved treatment of osteoporosis and obesity.