Neha Chaturvedi1, Sneha Bhandari1, Praveen K Bharti1, Sanjay K Basak2, Mrigendra P Singh3, Neeru Singh4. 1. National Institute for Research in Tribal Health (NIRTH) formerly known as Regional Medical Research Center for Tribals (RMRCT), ICMR, Nagpur Road, Garha, Jabalpur-482003, Madhya Pradesh, India. 2. District Malaria office & Govt. Maharani Hospital and Associated Medical College Jagdalpur, Chhattisgarh, India. 3. National Institute of Malaria Research Field Unit, Jabalpur, RMRCT Campus, Nagpur Road, Garha, Jabalpur, Madhya Prades, India. 4. National Institute for Research in Tribal Health (NIRTH) formerly known as Regional Medical Research Center for Tribals (RMRCT), ICMR, Nagpur Road, Garha, Jabalpur-482003, Madhya Pradesh, India neeru.singh@gmail.com.
Abstract
BACKGROUND: We report the first evidence of sympatric distribution of Plasmodium ovale curtisi and P. ovale wallikeri from India. METHODS: Fingerprick blood samples were collected from fever cases in district Bastar, Chhattisgarh State for malaria screening by microscopy and PCR. RESULTS: Two cases of mono infection of P. ovale, and a fatal case of cerebral malaria with a mixed infection of P. vivax, P. falciparum and P. ovale were confirmed by PCR. Sequencing analysis revealed the presence of P. ovale curtisi and P. ovale wallikeri. CONCLUSIONS: This study highlights the need of molecular diagnosis of malaria cases in forested areas for treatment and control.
BACKGROUND: We report the first evidence of sympatric distribution of Plasmodium ovale curtisi and P. ovale wallikeri from India. METHODS: Fingerprick blood samples were collected from fever cases in district Bastar, Chhattisgarh State for malaria screening by microscopy and PCR. RESULTS: Two cases of mono infection of P. ovale, and a fatal case of cerebral malaria with a mixed infection of P. vivax, P. falciparum and P. ovale were confirmed by PCR. Sequencing analysis revealed the presence of P. ovale curtisi and P. ovale wallikeri. CONCLUSIONS: This study highlights the need of molecular diagnosis of malaria cases in forested areas for treatment and control.