Li Ma1, Pan Sun1, Fangzhao Lin2, Hongjie Wang3, Xia Rong4, Yudong Dai5, Jianqiang Liu6, Liqiong Qian7, Min Fang8, Na Su1, Wei Xiao1, Shengliang Ye1, Changqing Li1. 1. Department of Blood Biochemistry, Institute of Blood Transfusion, Chinese Academy of Medical Sciences, Chengdu, China. 2. Department of Blood Biochemistry, Institute of Blood Transfusion, Chinese Academy of Medical Sciences, Chengdu, China lfangzhao@126.com. 3. Department of Blood Components, Blood Centre of the Beijing Red Cross, Beijing, China. 4. Department of Blood Components, Guangzhou Blood Centre, Guangzhou, China. 5. Department of Blood Components, Blood Centre of the Nanjing Red Cross, Nanjing, China. 6. Department of Blood Components, Shanxi Blood Centre, Xian, China. 7. Department of Blood Components, Deyang Central Blood Station, Deyang, China. 8. Department of Blood Components, Suzhou Central Blood Station, Suzhou, China.
Abstract
OBJECTIVE: To investigate the prevalence of hepatitis E virus (HEV) antibodies and RNA in voluntary blood donors, in China. METHODS: Serum samples from blood donors were collected sequentially at six blood donation centres in five geographical regions of China. Donors were stratified between two groups according to their serum alanine aminotransferase (ALT) concentration (≤ 40 U/l and >40 U/l. Enzyme-linked immunosorbent assay and reverse transcription-polymerase chain reaction were used to quantify anti-HEV antibodies and RNA, respectively. RESULTS: In total, 21.1% (172/816) of donors were positive for anti-HEV immunoglobulin (Ig) G (elevated ALT samples group 18.9% [85/450]; normal ALT group 23.8% [87/366]), and 0.5% (four of 816) donors were positive for anti-HEV IgM (elevated ALT group 0.9% [four of 450]; normal ALT group 0% [none of 366]). The rate of anti-HEV IgG seropositivity was significantly higher in male than in female donors. No serum sample tested positive for HEV RNA. CONCLUSION: The prevalence of anti-HEV antibodies in blood donors in China is high, regardless of ALT concentration.
OBJECTIVE: To investigate the prevalence of hepatitis E virus (HEV) antibodies and RNA in voluntary blood donors, in China. METHODS: Serum samples from blood donors were collected sequentially at six blood donation centres in five geographical regions of China. Donors were stratified between two groups according to their serum alanine aminotransferase (ALT) concentration (≤ 40 U/l and >40 U/l. Enzyme-linked immunosorbent assay and reverse transcription-polymerase chain reaction were used to quantify anti-HEV antibodies and RNA, respectively. RESULTS: In total, 21.1% (172/816) of donors were positive for anti-HEV immunoglobulin (Ig) G (elevated ALT samples group 18.9% [85/450]; normal ALT group 23.8% [87/366]), and 0.5% (four of 816) donors were positive for anti-HEV IgM (elevated ALT group 0.9% [four of 450]; normal ALT group 0% [none of 366]). The rate of anti-HEV IgG seropositivity was significantly higher in male than in female donors. No serum sample tested positive for HEV RNA. CONCLUSION: The prevalence of anti-HEV antibodies in blood donors in China is high, regardless of ALT concentration.
Authors: Ashish C Shrestha; Robert L P Flower; Clive R Seed; Manita Rajkarnikar; Shrawan K Shrestha; Uru Thapa; Veronica C Hoad; Helen M Faddy Journal: BMC Infect Dis Date: 2016-11-25 Impact factor: 3.090
Authors: Reem A Al Dossary; Awatif N Alnafie; Salma Ali Aljaroodi; Jawad Ur Rahman; Basavaraj C Hunasemarada; Khaled R Alkharsah Journal: J Multidiscip Healthc Date: 2021-08-27