| Literature DB >> 25704597 |
A C Ganime1, J P G Leite2, A de Abreu Corrêa3, F G Melgaço2, F A Carvalho-Costa2, M P Miagostovich2.
Abstract
The monitoring of virus contamination on fomites, especially at hospitals has been used for a more effective evaluation of the microbiological quality of surfaces. Swab sampling is the method used currently, although the use of an internal control process (ICP) has not yet been assessed. The aim of this study is to determine the recovery rate of murine norovirus 1 (MNV-1) and bacteriophage PP7 on different surfaces in order to assess their potential use as an ICP. For this purpose both viruses were spiked experimentally both on porous and non-porous formic as well as on rubberized surfaces. Quantitative PCR (qPCR) showed a variable efficiency with a percentage recovery ranging from 0.6 to 77% according to viruses and surfaces. A global analysis suggested that MNV-1 could be used as a potential ICP for the swab sampling method.Entities:
Keywords: Bacteriophage PP7; Fomites; Internal control process; MNV-1; Swab
Mesh：
Year: 2015 PMID： 25704597 DOI： 10.1016/j.jviromet.2015.02.009
Source DB: PubMed Journal: J Virol Methods ISSN： 0166-0934 Impact factor: 2.014