BACKGROUND: miR-23a, which participates in invasion of pancreatic ductal adenocarcinoma cells into the mesothelial barrier, is a critical regulator in many cancers. It, however, is still unknown whether miR-23a regulates pancreatic cell proliferation and apoptosis or not. AIMS: We sought to investigate the role of miR-23a in regulation of pancreatic cell proliferation and apoptosis. METHODS: miRNA, mRNA, and protein expressions were determined by qRT-PCR and Western blot, respectively. Dual-luciferase reporter assay was used in detection for binding ability of miR-23a to APAF1. Ectopic miR-23a and APAF 1 were introduced to pancreatic cells, and their roles in proliferation and apoptosis were detected by MTT, colony formation, and apoptosis assays, respectively. RESULTS: Up-regulation of miR-23a and down-regulation of APAF 1 were found in pancreatic ductal cancer, respectively. miR-23a significantly inhibited the luciferase activity by targeting APAF 1 3'UTR. Ectopic miR-23a significantly suppressed the APAF 1 gene expression in pancreatic cancer cells. Similar to siAPAF1, miR-23a significantly promoted pancreatic cancer cell proliferation and repressed apoptosis. Furthermore, miR-23a inhibitor and exogenous APAF 1 could recover the effects. CONCLUSIONS: It is suggested that miR-23a, acting as an oncogenic regulator by directly targeting APAF 1 in pancreatic cancer, is a useful potential biomarker in diagnosis and treatment of pancreatic cancer.
BACKGROUND:miR-23a, which participates in invasion of pancreatic ductal adenocarcinoma cells into the mesothelial barrier, is a critical regulator in many cancers. It, however, is still unknown whether miR-23a regulates pancreatic cell proliferation and apoptosis or not. AIMS: We sought to investigate the role of miR-23a in regulation of pancreatic cell proliferation and apoptosis. METHODS: miRNA, mRNA, and protein expressions were determined by qRT-PCR and Western blot, respectively. Dual-luciferase reporter assay was used in detection for binding ability of miR-23a to APAF1. Ectopic miR-23a and APAF 1 were introduced to pancreatic cells, and their roles in proliferation and apoptosis were detected by MTT, colony formation, and apoptosis assays, respectively. RESULTS: Up-regulation of miR-23a and down-regulation of APAF 1 were found in pancreatic ductal cancer, respectively. miR-23a significantly inhibited the luciferase activity by targeting APAF 1 3'UTR. Ectopic miR-23a significantly suppressed the APAF 1 gene expression in pancreatic cancer cells. Similar to siAPAF1, miR-23a significantly promoted pancreatic cancer cell proliferation and repressed apoptosis. Furthermore, miR-23a inhibitor and exogenous APAF 1 could recover the effects. CONCLUSIONS: It is suggested that miR-23a, acting as an oncogenic regulator by directly targeting APAF 1 in pancreatic cancer, is a useful potential biomarker in diagnosis and treatment of pancreatic cancer.
Authors: Jason W Hoskins; Jinping Jia; Marta Flandez; Hemang Parikh; Wenming Xiao; Irene Collins; Mickey A Emmanuel; Abdisamad Ibrahim; John Powell; Lizhi Zhang; Nuria Malats; William R Bamlet; Gloria M Petersen; Francisco X Real; Laufey T Amundadottir Journal: Carcinogenesis Date: 2014-09-18 Impact factor: 4.944
Authors: Wei-Neng Fu; Francesco Bertoni; Stephen M Kelsey; Suzanne M McElwaine; Finbarr E Cotter; Adrian C Newland; Li Jia Journal: Oncogene Date: 2003-01-23 Impact factor: 9.867
Authors: Shuangli Mi; Jun Lu; Miao Sun; Zejuan Li; Hao Zhang; Mary Beth Neilly; Yungui Wang; Zhijian Qian; Jie Jin; Yanming Zhang; Stefan K Bohlander; Michelle M Le Beau; Richard A Larson; Todd R Golub; Janet D Rowley; Jianjun Chen Journal: Proc Natl Acad Sci U S A Date: 2007-12-04 Impact factor: 11.205
Authors: Jennifer Permuth-Wey; Y Ann Chen; Kate Fisher; Susan McCarthy; Xiaotao Qu; Mark C Lloyd; Agnieszka Kasprzak; Michelle Fournier; Vonetta L Williams; Kavita M Ghia; Sean J Yoder; Laura Hall; Christina Georgeades; Funmilayo Olaoye; Kazim Husain; Gregory M Springett; Dung-Tsa Chen; Timothy Yeatman; Barbara Ann Centeno; Jason Klapman; Domenico Coppola; Mokenge Malafa Journal: PLoS One Date: 2015-01-21 Impact factor: 3.240