Iradj Ashrafi Tamai1, Taghi Zahraei Salehi2, Aghil Sharifzadeh3, Hojjatollah Shokri4, Ali Reza Khosravi3. 1. Bu Ali Sina University, Hamedan, Iran ; Research and Training Center of Imam Khomeini Hospital, Tehran, Iran. 2. Department of Microbiology and Immunology, Faculty of Veterinary Medicine, University of Tehran, Tehran, Iran. 3. Mycology Research Center, Faculty of Veterinary Medicine, University of Tehran, Tehran, Iran. 4. Faculty of Veterinary Medicine, Amol University of Special Modern Technologies, Amol, Iran.
Abstract
OBJECTIVES: Candidiasis infection caused by Candida albicans has been known as a major problem in patients with immune disorders. The objective of this study was to genotype the C. albicans isolates obtained from oral cavity of patients with positive human immunodeficiency virus (HIV(+)) with or/and without oropharyngeal candidiasis (OPC). MATERIALS AND METHODS: A total of 100 C. albicans isolates from Iranian HIV(+)patients were genotyped using specific PCR primers of the 25S rDNA and RPS genes. RESULTS: The frequencies of genotypes A, B and C which were achieved using 25S rDNA , were 66, 24 and 10 percent, respectively. In addition, genotypes D and E were not found in this study. Each C. albicans genotype was further classified into four subtypes (types 2, 3, 2/3 and 3/4) by PCR amplification targeting RPS sequence. CONCLUSION: In general, genotype A3 constituted the majority of understudy clinical isolates obtained from oral cavity of Iranian HIV(+) patients.
OBJECTIVES:Candidiasis infection caused by Candida albicans has been known as a major problem in patients with immune disorders. The objective of this study was to genotype the C. albicans isolates obtained from oral cavity of patients with positive human immunodeficiency virus (HIV(+)) with or/and without oropharyngeal candidiasis (OPC). MATERIALS AND METHODS: A total of 100 C. albicans isolates from Iranian HIV(+)patients were genotyped using specific PCR primers of the 25S rDNA and RPS genes. RESULTS: The frequencies of genotypes A, B and C which were achieved using 25S rDNA , were 66, 24 and 10 percent, respectively. In addition, genotypes D and E were not found in this study. Each C. albicans genotype was further classified into four subtypes (types 2, 3, 2/3 and 3/4) by PCR amplification targeting RPS sequence. CONCLUSION: In general, genotype A3 constituted the majority of understudy clinical isolates obtained from oral cavity of Iranian HIV(+) patients.
Authors: Patindoilba Marcel Sawadogo; Adama Zida; Issiaka Soulama; S Samuel Sermé; Kiswendsida Thierry Guiguemdé; Richard Junior; Ibrahim Sangaré; Sanata Bamba; Rasmata Ouédraogo-Traoré; Tinga Robert Guiguemdé Journal: Infect Drug Resist Date: 2019-12-16 Impact factor: 4.003