BACKGROUND: Several molecular biology-based genotyping techniques have been adapted for studying the molecular characteristics of Candida albicans strains, which constitute the majority of the etiologic agents in candidiasis. Recently, we reported a PCR system targeting 25S rDNA and ALT repeat sequences in the repetitive sequence (RPS) for genotyping of C. albicans. OBJECTIVE: To assess the potential of 25S rDNA and RPS-based genotyping for studying the molecular epidemiology of C. albicans, and define the genotypic relationship of C. albicans between invasive and non-invasive lesions in the same individual. METHODS: C. albicans strains were isolated from infected lesions and commensal sites, such as oral mucosa and/or feces, of patients with superficial candidiasis. The genomic DNAs were amplified by PCRs using P-I and P-II to determine the 25S rDNA- and RPS-based genotypes of the isolates. RESULTS: Genotype A:3 C. albicans constituted the majority of the isolates, followed by A:3/4 and B:3 C. albicans. There was usually one genotype of C. albicans per person. The genotypes of infected lesion isolates and non-infected oral mucosa and/or feces isolates were identical in the same individual, even in serially isolated C. albicans. CONCLUSION: The results indicate that our combined PCR technique using P-I and P-II is a potential tool for molecular typing of C. albicans, and reveal that the genotypes of isolates are identical in the same individual, independent of the infective and non-infective phases or the body location.
BACKGROUND: Several molecular biology-based genotyping techniques have been adapted for studying the molecular characteristics of Candida albicans strains, which constitute the majority of the etiologic agents in candidiasis. Recently, we reported a PCR system targeting 25S rDNA and ALT repeat sequences in the repetitive sequence (RPS) for genotyping of C. albicans. OBJECTIVE: To assess the potential of 25S rDNA and RPS-based genotyping for studying the molecular epidemiology of C. albicans, and define the genotypic relationship of C. albicans between invasive and non-invasive lesions in the same individual. METHODS:C. albicans strains were isolated from infected lesions and commensal sites, such as oral mucosa and/or feces, of patients with superficial candidiasis. The genomic DNAs were amplified by PCRs using P-I and P-II to determine the 25S rDNA- and RPS-based genotypes of the isolates. RESULTS: Genotype A:3 C. albicans constituted the majority of the isolates, followed by A:3/4 and B:3 C. albicans. There was usually one genotype of C. albicans per person. The genotypes of infected lesion isolates and non-infected oral mucosa and/or feces isolates were identical in the same individual, even in serially isolated C. albicans. CONCLUSION: The results indicate that our combined PCR technique using P-I and P-II is a potential tool for molecular typing of C. albicans, and reveal that the genotypes of isolates are identical in the same individual, independent of the infective and non-infective phases or the body location.
Authors: R H Jensen; H K Johansen; L M Søes; L E Lemming; F S Rosenvinge; L Nielsen; B Olesen; L Kristensen; E Dzajic; K M T Astvad; M C Arendrup Journal: Antimicrob Agents Chemother Date: 2015-12-28 Impact factor: 5.191
Authors: Katarína Mlynáriková; Ota Samek; Silvie Bernatová; Filip Růžička; Jan Ježek; Andrea Hároniková; Martin Šiler; Pavel Zemánek; Veronika Holá Journal: Sensors (Basel) Date: 2015-11-24 Impact factor: 3.576
Authors: Patindoilba Marcel Sawadogo; Adama Zida; Issiaka Soulama; S Samuel Sermé; Kiswendsida Thierry Guiguemdé; Richard Junior; Ibrahim Sangaré; Sanata Bamba; Rasmata Ouédraogo-Traoré; Tinga Robert Guiguemdé Journal: Infect Drug Resist Date: 2019-12-16 Impact factor: 4.003