D Fonmarty1, S Cherrière1, H Fleury2, S Eimer3, C Majoufre-Lefebvre4, V Castetbon1, E de Monès5. 1. Service d'oto-rhino-laryngologie, CHU de Bordeaux, Hôpital Pellegrin, place Amélie-Raba-Léon, 33056 Bordeaux cedex, France. 2. Laboratoire de virologie, CHU de Bordeaux, Hôpital Pellegrin, place Amélie-Raba-Léon, 33056 Bordeaux cedex, France. 3. Laboratoire d'anatomie-pathologique, CHU de Bordeaux, Hôpital Pellegrin, place Amélie-Raba-Léon, 33056 Bordeaux cedex, France. 4. Service de chirurgie maxillo-faciale, CHU de Bordeaux, Hôpital Pellegrin, place Amélie-Raba-Léon, 33056 Bordeaux cedex, France. 5. Service d'oto-rhino-laryngologie, CHU de Bordeaux, Hôpital Pellegrin, place Amélie-Raba-Léon, 33056 Bordeaux cedex, France. Electronic address: erwan.de-mones-del-pujol@chu-bordeaux.fr.
Abstract
OBJECTIVE: The diagnosis of HPV-related oropharyngeal cancer in clinical practice is based on p16 immunohistochemistry and PCR detection of viral DNA (HPV-PCR). The primary objective of this study was to evaluate the concordance between these 2 diagnostic tests. The secondary objective was to study the clinical characteristics of these patients. MATERIALS AND METHODS: This single-centre prospective study was conducted between February 2010 and July 2012. Immunohistochemical analysis of p16 and HPV-PCR were performed on tumour biopsies. Concordance was evaluated according to Cohen's kappa coefficient and was interpreted according to the Landis and Koch scale. The patients' clinical data were analysed as a function of the diagnostic test results. RESULTS: Seventy-one patients were included in this study. The prevalence of HPV was 43.7% according to p16 and 31% according to HPV-PCR. The concordance study revealed a kappa coefficient of 0.615. A tumour of the tonsil or base of the tongue was detected in 100% of p16+/HPV-PCR+ cases. Smoking and alcohol abuse were significantly less frequent among HPV+ patients regardless of the method of detection. These patients were older and presented tumours with a lower grade of histological differentiation. CONCLUSION: p16 immunohistochemistry or HPV-PCR used alone appear to be insufficient. These results confirm the high prevalence of HPV-related oropharyngeal squamous cell carcinoma (OSCC) and the previously reported specific clinical and histological features, apart from age. It appears essential for future clinical trials to be stratified according to smoking and tumour HPV status, defined by means of reliable virological tests targeting E6/E7 mRNA and no longer a simple positive response to the p16 marker, as is frequently the case at the present time. New tests suitable for use in routine practice therefore need to be developed.
OBJECTIVE: The diagnosis of HPV-related oropharyngeal cancer in clinical practice is based on p16 immunohistochemistry and PCR detection of viral DNA (HPV-PCR). The primary objective of this study was to evaluate the concordance between these 2 diagnostic tests. The secondary objective was to study the clinical characteristics of these patients. MATERIALS AND METHODS: This single-centre prospective study was conducted between February 2010 and July 2012. Immunohistochemical analysis of p16 and HPV-PCR were performed on tumour biopsies. Concordance was evaluated according to Cohen's kappa coefficient and was interpreted according to the Landis and Koch scale. The patients' clinical data were analysed as a function of the diagnostic test results. RESULTS: Seventy-one patients were included in this study. The prevalence of HPV was 43.7% according to p16 and 31% according to HPV-PCR. The concordance study revealed a kappa coefficient of 0.615. A tumour of the tonsil or base of the tongue was detected in 100% of p16+/HPV-PCR+ cases. Smoking and alcohol abuse were significantly less frequent among HPV+ patients regardless of the method of detection. These patients were older and presented tumours with a lower grade of histological differentiation. CONCLUSION:p16 immunohistochemistry or HPV-PCR used alone appear to be insufficient. These results confirm the high prevalence of HPV-related oropharyngeal squamous cell carcinoma (OSCC) and the previously reported specific clinical and histological features, apart from age. It appears essential for future clinical trials to be stratified according to smoking and tumour HPV status, defined by means of reliable virological tests targeting E6/E7 mRNA and no longer a simple positive response to the p16 marker, as is frequently the case at the present time. New tests suitable for use in routine practice therefore need to be developed.
Authors: Paula Roberta Aguiar Pastrez; Vânia Sammartino Mariano; Allini Mafra da Costa; Estela Maria Silva; Cristovam Scapulatempo-Neto; Denise Peixoto Guimarães; Gilberto Fava; Said Abdala Zemi Neto; Emily Montosa Nunes; Laura Sichero; Luisa Lina Villa; Kari Juhani Syrjanen; Adhemar Longatto-Filho Journal: J Cancer Date: 2017-04-09 Impact factor: 4.207
Authors: Ryan Langdon; Rebecca Richmond; Hannah R Elliott; Tom Dudding; Nabila Kazmi; Chris Penfold; Kate Ingarfield; Karen Ho; Andrew Bretherick; Chris Haley; Yanni Zeng; Rosie M Walker; Michael Pawlita; Tim Waterboer; Tom Gaunt; George Davey Smith; Matthew Suderman; Steve Thomas; Andy Ness; Caroline Relton Journal: Clin Epigenetics Date: 2020-06-29 Impact factor: 6.551
Authors: Rachel F Shenker; Nelson H May; Joshua D Waltonen; Jae Paul Yang; Stacey S O'Neill; Bart A Frizzell; Kathryn M Greven; Ryan T Hughes Journal: Head Neck Pathol Date: 2021-02-22