| Literature DB >> 25669885 |
Barbara Di Fiore1, Norman E Davey2,3, Anja Hagting1, Daisuke Izawa1, Jörg Mansfeld4, Toby J Gibson3, Jonathon Pines1.
Abstract
The anaphase-promoting complex or cyclosome (APC/C) is the ubiquitin ligase that regulates mitosis by targeting specific proteins for degradation at specific times under the control of the spindle assembly checkpoint (SAC). How the APC/C recognizes its different substrates is a key problem in the control of cell division. Here, we have identified the ABBA motif in cyclin A, BUBR1, BUB1, and Acm1, and we show that it binds to the APC/C coactivator CDC20. The ABBA motif in cyclin A is required for its proper degradation in prometaphase through competing with BUBR1 for the same site on CDC20. Moreover, the ABBA motifs in BUBR1 and BUB1 are necessary for the SAC to work at full strength and to recruit CDC20 to kinetochores. Thus, we have identified a conserved motif integral to the proper control of mitosis that connects APC/C substrate recognition with the SAC.Entities:
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Year: 2015 PMID： 25669885 PMCID： PMC4713905 DOI： 10.1016/j.devcel.2015.01.003
Source DB: PubMed Journal: Dev Cell ISSN： 1534-5807 Impact factor: 12.270