Yukihiko Hiroshima1, Yong Zhang2, Nan Zhang2, Fuminari Uehara3, Ali Maawy4, Takashi Murakami3, Sumiyuki Mii3, Mako Yamamoto3, Shinji Miwa3, Shuya Yano3, Masashi Momiyama5, Ryutaro Mori5, Ryusei Matsuyama5, Takashi Chishima5, Kuniya Tanaka5, Yasushi Ichikawa5, Michael Bouvet4, Itaru Endo5, Robert M Hoffman6. 1. AntiCancer, Inc., San Diego, CA, U.S.A. Department of Surgery, University of California San Diego, San Diego, CA, U.S.A. Department of Gastroenterological Surgery, Yokohama City University Graduate School of Medicine, Yokohama, Japan. 2. AntiCancer, Inc., San Diego, CA, U.S.A. 3. AntiCancer, Inc., San Diego, CA, U.S.A. Department of Surgery, University of California San Diego, San Diego, CA, U.S.A. 4. Department of Surgery, University of California San Diego, San Diego, CA, U.S.A. 5. Department of Gastroenterological Surgery, Yokohama City University Graduate School of Medicine, Yokohama, Japan. 6. AntiCancer, Inc., San Diego, CA, U.S.A. Department of Surgery, University of California San Diego, San Diego, CA, U.S.A. all@anticancer.com.
Abstract
AIM: Soft-tissue sarcomas are a group of rare mesenchymal carcinomas that include approximately 50 histological types, and account for 1% of all adult cancer cases. The yearly incidence of soft-tissue sarcomas in the USA is approximately 11,280 cases, with an overall mortality of 3,900 deaths per year. MATERIALS AND METHODS: In this study, we established a patient-derived orthotopic xenograft (PDOX) from a patient with a soft-tissue sarcoma of the retroperitoneum in nude mice and compared it to a subcutaneous patient-derived model of the same tumor for histology. RESULTS: In the PDOX model, a bulky tumor grew in the left retroperitoneum in the same manner as the patient's tumor. Upon histological examination, the majority of the PDOX tissue section comprised sarcomatous high-grade spindle cells of varying sizes, similar to the original patient tumor. In contrast, the majority of the subcutaneously-implanted tumor comprised round to oval cells. CONCLUSION: These results indicate that the PDOX recapitulated the histology of the original tumor more than the subcutaneous model. Copyright
AIM: Soft-tissue sarcomas are a group of rare mesenchymal carcinomas that include approximately 50 histological types, and account for 1% of all adult cancer cases. The yearly incidence of soft-tissue sarcomas in the USA is approximately 11,280 cases, with an overall mortality of 3,900 deaths per year. MATERIALS AND METHODS: In this study, we established a patient-derived orthotopic xenograft (PDOX) from a patient with a soft-tissue sarcoma of the retroperitoneum in nude mice and compared it to a subcutaneous patient-derived model of the same tumor for histology. RESULTS: In the PDOX model, a bulky tumor grew in the left retroperitoneum in the same manner as the patient's tumor. Upon histological examination, the majority of the PDOX tissue section comprised sarcomatous high-grade spindle cells of varying sizes, similar to the original patienttumor. In contrast, the majority of the subcutaneously-implanted tumor comprised round to oval cells. CONCLUSION: These results indicate that the PDOX recapitulated the histology of the original tumor more than the subcutaneous model. Copyright
Authors: Kei Kawaguchi; Qinghong Han; Shukuan Li; Yuying Tan; Kentaro Igarashi; Tasuku Kiyuna; Kentaro Miyake; Masuyo Miyake; Bartosz Chmielowski; Scott D Nelson; Tara A Russell; Sarah M Dry; Yunfeng Li; Arun S Singh; Mark A Eckardt; Michiaki Unno; Fritz C Eilber; Robert M Hoffman Journal: Cell Cycle Date: 2018-03-19 Impact factor: 4.534
Authors: Kei Kawaguchi; Kentaro Igarashi; Takashi Murakami; Tasuku Kiyuna; Scott D Nelson; Sarah M Dry; Yunfeng Li; Tara A Russell; Arun S Singh; Bartosz Chmielowski; Michiaki Unno; Fritz C Eilber; Robert M Hoffman Journal: Cell Cycle Date: 2017-04-20 Impact factor: 4.534
Authors: Christopher G England; Dawei Jiang; Reinier Hernandez; Haiyan Sun; Hector F Valdovinos; Emily B Ehlerding; Jonathan W Engle; Yunan Yang; Peng Huang; Weibo Cai Journal: Mol Pharm Date: 2017-09-06 Impact factor: 4.939
Authors: Kentaro Igarashi; Kei Kawaguchi; Takashi Murakami; Tasuku Kiyuna; Kentaro Miyake; Scott D Nelson; Sarah M Dry; Yunfeng Li; Jane Yanagawa; Tara A Russell; Arun S Singh; Norio Yamamoto; Katsuhiro Hayashi; Hiroaki Kimura; Shinji Miwa; Hiroyuki Tsuchiya; Fritz C Eilber; Robert M Hoffman Journal: Cell Cycle Date: 2017-05-11 Impact factor: 4.534
Authors: Takashi Murakami; Tasuku Kiyuna; Kei Kawaguchi; Kentaro Igarashi; Arun S Singh; Yukihiko Hiroshima; Yong Zhang; Ming Zhao; Kentaro Miyake; Scott D Nelson; Sarah M Dry; Yunfeng Li; Jonathan C DeLong; Thinzar M Lwin; Takashi Chishima; Kuniya Tanaka; Michael Bouvet; Itaru Endo; Fritz C Eilber; Robert M Hoffman Journal: Cell Cycle Date: 2017-03-15 Impact factor: 4.534
Authors: Kentaro Igarashi; Kei Kawaguchi; Tasuku Kiyuna; Kentaro Miyake; Masuyo Miyake; Shukuan Li; Qinghong Han; Yuying Tan; Ming Zhao; Yunfeng Li; Scott D Nelson; Sarah M Dry; Arun S Singh; Irmina A Elliott; Tara A Russell; Mark A Eckardt; Norio Yamamoto; Katsuhiro Hayashi; Hiroaki Kimura; Shinji Miwa; Hiroyuki Tsuchiya; Fritz C Eilber; Robert M Hoffman Journal: Cell Cycle Date: 2018-04-10 Impact factor: 4.534
Authors: Kei Kawaguchi; Kentaro Igarashi; Tasuku Kiyuna; Kentaro Miyake; Masuyo Miyake; Takashi Murakami; Bartosz Chmielowski; Scott D Nelson; Tara A Russell; Sarah M Dry; Yunfeng Li; Arun S Singh; Michiaki Unno; Fritz C Eilber; Robert M Hoffman Journal: Cell Cycle Date: 2018-03-29 Impact factor: 4.534