Jonathan W Riess1, Robert West2, Michelle Dean3, Alex C Klimowicz3, Joel W Neal4, Chuong Hoang5, Heather A Wakelee4. 1. Department of Medicine, Division of Hematology/Oncology, University of California Davis Comprehensive Cancer Center, University of California Davis School of Medicine, Sacramento, CA, U.S.A. Department of Medicine, Division of Oncology, Stanford University School of Medicine, Stanford, CA, U.S.A. jonathan.riess@udmc.ucdavis.edu. 2. Department of Pathology, Division of Thoracic Surgery, Stanford University School of Medicine, Stanford, CA, U.S.A. 3. Tom Baker Cancer Centre, Translational Research Laboratories, Alberta Health Services and Southern Alberta Cancer Research Institute, Alberta, Canada. 4. Department of Medicine, Division of Oncology, Stanford University School of Medicine, Stanford, CA, U.S.A. 5. Department of Surgery, Division of Thoracic Surgery, Stanford University School of Medicine, Stanford, CA, U.S.A.
Abstract
BACKGROUND/AIM: Thymic epithelial tumors (TET) are rare. Wingless and INT (WNT), NOTCH and sonic hedgehog pathway interactions between thymocytes and thymic stroma are important to thymus and T-cell development. We analyzed a thymoma tissue microarray (TMA) for glioma associated oncogene homolog 1 (GLI1), NOTCH1 and catenin (cadherin-associated protein, beta 1) (CTNNB1) expression as surrogate markers of sonic hedgehog, NOTCH and WNT pathway activity. MATERIALS AND METHODS: GLI1, NOTCH1 and CTNNB1 expression were assayed in a tissue microarray of 68 TET and eight benign thymus by fluorescent immunohistochemistry (AQUA) as surrogates for activity of the sonic hedgehog, NOTCH and WNT pathways respectively. RESULTS: No difference in tumor GLI1 (mean 201 vs. 211, p=0.31), CTNNB1 (mean 222 vs. 306, p=0.66) or NOTCH1 expression (mean 317 vs. 325, p=0.82) was noted between thymic tumor and benign thymus. CONCLUSION: No evidence for preferential expression of GLI1, NOTCH1 or CTNNB1 was noted. High-throughput immunofluorescence using AQUA technology can help overcome limitations of small sample size and tissue heterogeneity when analyzing protein expression in thymic tumors. Copyright
BACKGROUND/AIM: Thymic epithelial tumors (TET) are rare. Wingless and INT (WNT), NOTCH and sonic hedgehog pathway interactions between thymocytes and thymic stroma are important to thymus and T-cell development. We analyzed a thymoma tissue microarray (TMA) for glioma associated oncogene homolog 1 (GLI1), NOTCH1 and catenin (cadherin-associated protein, beta 1) (CTNNB1) expression as surrogate markers of sonic hedgehog, NOTCH and WNT pathway activity. MATERIALS AND METHODS:GLI1, NOTCH1 and CTNNB1 expression were assayed in a tissue microarray of 68 TET and eight benign thymus by fluorescent immunohistochemistry (AQUA) as surrogates for activity of the sonic hedgehog, NOTCH and WNT pathways respectively. RESULTS: No difference in tumorGLI1 (mean 201 vs. 211, p=0.31), CTNNB1 (mean 222 vs. 306, p=0.66) or NOTCH1 expression (mean 317 vs. 325, p=0.82) was noted between thymic tumor and benign thymus. CONCLUSION: No evidence for preferential expression of GLI1, NOTCH1 or CTNNB1 was noted. High-throughput immunofluorescence using AQUA technology can help overcome limitations of small sample size and tissue heterogeneity when analyzing protein expression in thymic tumors. Copyright
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