BACKGROUND: The infection route of Helicobacter pylori has been recognized to be mainly intrafamilial, preferentially mother-to-child, especially in developed countries. To determine the transmission route, we examined whether multilocus sequence typing (MLST) was useful for analysis of intrafamilial infection. The possibility of intraspousal infection was also evaluated. MATERIALS AND METHODS: Clonal relationships between strains derived from 35 index Japanese pediatric patients, and their family members were analyzed by two genetic typing procedures, MLST and random amplified polymorphic DNA (RAPD) fingerprinting. RESULTS: Mostly coincident results were obtained by MLST and RAPD. By MLST, the allele of loci in the isolates mostly matched between the index child and both the father and mother for 9 (25.7%) of the 35 patients, between the index child and the mother for 25 (60.0%) of the 35 patients. CONCLUSIONS: MLST is useful for analyzing the infection route of H. pylori as a highly reproducible method. Intrafamilial, especially mother-to-children and sibling, infection is the dominant transmission route. Intraspousal infection is also thought to occur in about a quarter in the Japanese families.
BACKGROUND: The infection route of Helicobacter pylori has been recognized to be mainly intrafamilial, preferentially mother-to-child, especially in developed countries. To determine the transmission route, we examined whether multilocus sequence typing (MLST) was useful for analysis of intrafamilial infection. The possibility of intraspousal infection was also evaluated. MATERIALS AND METHODS: Clonal relationships between strains derived from 35 index Japanese pediatric patients, and their family members were analyzed by two genetic typing procedures, MLST and random amplified polymorphic DNA (RAPD) fingerprinting. RESULTS: Mostly coincident results were obtained by MLST and RAPD. By MLST, the allele of loci in the isolates mostly matched between the index child and both the father and mother for 9 (25.7%) of the 35 patients, between the index child and the mother for 25 (60.0%) of the 35 patients. CONCLUSIONS: MLST is useful for analyzing the infection route of H. pylori as a highly reproducible method. Intrafamilial, especially mother-to-children and sibling, infection is the dominant transmission route. Intraspousal infection is also thought to occur in about a quarter in the Japanese families.
Authors: Hashem B El-Serag; John Y Kao; Fasiha Kanwal; Mark Gilger; Frank LoVecchio; Steven F Moss; Sheila E Crowe; Adam Elfant; Thomas Haas; Ronald J Hapke; David Y Graham Journal: Clin Gastroenterol Hepatol Date: 2018-03-17 Impact factor: 11.382
Authors: Alejandra Mendoza-Cantú; Víctor Hugo Urrutia-Baca; Cynthia Sofía Urbina-Ríos; Myriam Angélica De la Garza-Ramos; Martha Elena García-Martínez; Hilda H H Torre-Martínez Journal: Biomed Res Int Date: 2017-11-01 Impact factor: 3.411
Authors: Mohammad A Abu-Lubad; Ghada F Helaly; Weliam J Haddadin; Dua'a A K Jarajreh; Amin A Aqel; Munir A Al-Zeer Journal: Int J Microbiol Date: 2022-03-23
Authors: Selma Maluf; João Victor Salgado; Dalila Nunes Cysne; Daniel Monte Freire Camelo; Johnny Ramos Nascimento; Bianca Vitória T Maluf; Luís Douglas Miranda Silva; Marta Regina de Castro Belfort; Lucilene Amorim Silva; Rosane Nassar Meireles Guerra; Natalino Salgado Filho; Flávia Raquel F Nascimento Journal: Front Immunol Date: 2020-09-10 Impact factor: 7.561