Literature DB >> 25664797

Crystallization and preliminary X-ray crystallographic studies of dipeptidyl peptidase 11 from Porphyromonas gingivalis.

Yasumitsu Sakamoto1, Yoshiyuki Suzuki2, Ippei Iizuka1, Chika Tateoka1, Saori Roppongi1, Mayu Fujimoto1, Hiroaki Gouda3, Takamasa Nonaka1, Wataru Ogasawara2, Nobutada Tanaka3.   

Abstract

Dipeptidyl peptidase 11 from Porphyromonas gingivalis (PgDPP11) preferentially cleaves substrate peptides with Asp and Glu at the P1 position [NH2-P2-P1(Asp/Glu)-P1'-P2'...]. For crystallographic studies, PgDPP11 was overproduced in Escherichia coli, purified and crystallized using the hanging-drop vapour-diffusion method. X-ray diffraction data to 1.82 Å resolution were collected from an orthorhombic crystal form belonging to space group C2221, with unit-cell parameters a = 99.33, b = 103.60, c = 177.33 Å. Structural analysis by the multi-wavelength anomalous diffraction method is in progress.

Entities:  

Keywords:  DPP11; Porphyromonas gingivalis; dipeptidyl peptidase

Mesh:

Substances:

Year:  2015        PMID: 25664797      PMCID: PMC4321477          DOI: 10.1107/S2053230X15000424

Source DB:  PubMed          Journal:  Acta Crystallogr F Struct Biol Commun        ISSN: 2053-230X            Impact factor:   1.056


  22 in total

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Authors:  A Banbula; J Yen; A Oleksy; P Mak; M Bugno; J Travis; J Potempa
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3.  Preferential utilization of dipeptides by Porphyromonas gingivalis.

Authors:  N Takahashi; T Sato
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4.  Solvent content of protein crystals.

Authors:  B W Matthews
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5.  Metabolic pathways for cytotoxic end product formation from glutamate- and aspartate-containing peptides by Porphyromonas gingivalis.

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Journal:  J Bacteriol       Date:  2000-09       Impact factor: 3.490

6.  Selenomethionyl proteins produced for analysis by multiwavelength anomalous diffraction (MAD): a vehicle for direct determination of three-dimensional structure.

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  1 in total

1.  Structural and mutational analyses of dipeptidyl peptidase 11 from Porphyromonas gingivalis reveal the molecular basis for strict substrate specificity.

Authors:  Yasumitsu Sakamoto; Yoshiyuki Suzuki; Ippei Iizuka; Chika Tateoka; Saori Roppongi; Mayu Fujimoto; Koji Inaka; Hiroaki Tanaka; Mitsugu Yamada; Kazunori Ohta; Hiroaki Gouda; Takamasa Nonaka; Wataru Ogasawara; Nobutada Tanaka
Journal:  Sci Rep       Date:  2015-06-09       Impact factor: 4.379

  1 in total

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