OBJECTIVES: This study was designed to evaluate the effects of chronic cyclosporine A (CsA) treatment and acute renal ischaemia/reperfusion (I/R) on the kidney and liver in thymoquinone (TQ)-treated rats. METHODS: In the CsA study, adult male rats were divided into control, CsA (25 mg/kg per day), TQ (10 mg/kg per day) and CsA + TQ groups, and rat treatment was for 28 days. In the I/R study, adult male rats were divided into sham-operated, I/R (renal ischaemia for 60 min followed by 60 min reperfusion) and TQ + I/R (TQ 10 mg/kg, 24 h and 1 h before ischaemia) groups. KEY FINDINGS: CsA treatment and renal I/R caused kidney and liver dysfunction as evaluated by histopathological changes and biochemical parameters. TQ treatment reduced elevated serum indices back to control levels and ameliorated CsA-induced kidney and liver histopathological changes. In renal and hepatic tissues, CsA and renal I/R induced significant increases in malondialdehyde levels with significant decreases in reduced glutathione levels and superoxide dismutase activities. Such changes in oxidative stress markers were counteracted by TQ treatment. CONCLUSIONS: Kidney and liver injury due to CsA or renal I/R can be significantly reduced by TQ, which resets the oxidant/antioxidant balance of the affected organs through scavenging free radicals and antilipoperoxidative effects.
OBJECTIVES: This study was designed to evaluate the effects of chronic cyclosporine A (CsA) treatment and acute renal ischaemia/reperfusion (I/R) on the kidney and liver in thymoquinone (TQ)-treated rats. METHODS: In the CsA study, adult male rats were divided into control, CsA (25 mg/kg per day), TQ (10 mg/kg per day) and CsA + TQ groups, and rat treatment was for 28 days. In the I/R study, adult male rats were divided into sham-operated, I/R (renal ischaemia for 60 min followed by 60 min reperfusion) and TQ + I/R (TQ 10 mg/kg, 24 h and 1 h before ischaemia) groups. KEY FINDINGS:CsA treatment and renal I/R caused kidney and liver dysfunction as evaluated by histopathological changes and biochemical parameters. TQ treatment reduced elevated serum indices back to control levels and ameliorated CsA-induced kidney and liver histopathological changes. In renal and hepatic tissues, CsA and renal I/R induced significant increases in malondialdehyde levels with significant decreases in reduced glutathione levels and superoxide dismutase activities. Such changes in oxidative stress markers were counteracted by TQ treatment. CONCLUSIONS: Kidney and liver injury due to CsA or renal I/R can be significantly reduced by TQ, which resets the oxidant/antioxidant balance of the affected organs through scavenging free radicals and antilipoperoxidative effects.
Authors: Basma Salama; Khalid J Alzahrani; Khalid S Alghamdi; Osama Al-Amer; Khalid E Hassan; Mohamed A Elhefny; Alaa Jameel A Albarakati; Fahad Alharthi; Hussam A Althagafi; Hassan Al Sberi; Hatem K Amin; Maha S Lokman; Khalaf F Alsharif; Ashraf Albrakati; Ahmed E Abdel Moneim; Rami B Kassab; Ayah S Fathalla Journal: Biol Trace Elem Res Date: 2022-08-26 Impact factor: 4.081
Authors: Şaban Cem Sezen; Aysegul Kucuk; Abdullah Özer; Yiğit Kılıç; Barış Mardin; Metin Alkan; Fatmanur Duruk Erkent; Mustafa Arslan; Yusuf Ünal; Gürsel Levent Oktar; Murat Tosun Journal: Drug Des Devel Ther Date: 2018-05-22 Impact factor: 4.319