Warning: Undefined array key "mm" in /www/wwwroot/www.ai-bt.com/si.php on line 10 Deprecated: trim(): Passing null to parameter #1 ($string) of type string is deprecated in /www/wwwroot/www.ai-bt.com/si.php on line 10 Real-time deformability cytometry: on-the-fly cell mechanical phenotyping.

Literature DB >> 25643151

Real-time deformability cytometry: on-the-fly cell mechanical phenotyping.

Oliver Otto¹, Philipp Rosendahl¹, Alexander Mietke¹, Stefan Golfier¹, Christoph Herold¹, Daniel Klaue¹, Salvatore Girardo¹, Stefano Pagliara², Andrew Ekpenyong³, Angela Jacobi¹, Manja Wobus⁴, Nicole Töpfner⁵, Ulrich F Keyser², Jörg Mansfeld¹, Elisabeth Fischer-Friedrich⁶, Jochen Guck⁷.

Abstract

We introduce real-time deformability cytometry (RT-DC) for continuous cell mechanical characterization of large populations (>100,000 cells) with analysis rates greater than 100 cells/s. RT-DC is sensitive to cytoskeletal alterations and can distinguish cell-cycle phases, track stem cell differentiation into distinct lineages and identify cell populations in whole blood by their mechanical fingerprints. This technique adds a new marker-free dimension to flow cytometry with diverse applications in biology, biotechnology and medicine.

Mesh：

Substances：

Year: 2015 PMID： 25643151 DOI： 10.1038/nmeth.3281

Source DB: PubMed Journal: Nat Methods ISSN： 1548-7091 Impact factor: 28.547

26 in total

Review 1. Mitotic spindle assembly and chromosome segregation: refocusing on microtubule dynamics.

Authors: Susan L Kline-Smith; Claire E Walczak
Journal: Mol Cell Date: 2004-08-13 Impact factor: 17.970

2. Sorting cells by size, shape and deformability.

Authors: Jason P Beech; Stefan H Holm; Karl Adolfsson; Jonas O Tegenfeldt
Journal: Lab Chip Date: 2012-02-10 Impact factor: 6.799

Review 3. The actin cytoskeleton in spindle assembly and positioning.

Authors: Patricia Kunda; Buzz Baum
Journal: Trends Cell Biol Date: 2009-03-13 Impact factor: 20.808

4. Viscoelasticity as a biomarker for high-throughput flow cytometry.

Authors: Tobias Sawetzki; Charles D Eggleton; Sanjay A Desai; David W M Marr
Journal: Biophys J Date: 2013-11-19 Impact factor: 4.033

5. The regulatory role of cell mechanics for migration of differentiating myeloid cells.

Authors: Franziska Lautenschläger; Stephan Paschke; Stefan Schinkinger; Arlette Bruel; Michael Beil; Jochen Guck
Journal: Proc Natl Acad Sci U S A Date: 2009-08-26 Impact factor: 11.205

6. Deformation of red blood cells in capillaries.

Authors: R Skalak; P I Branemark
Journal: Science Date: 1969-05-09 Impact factor: 47.728

7. Pinched-flow hydrodynamic stretching of single-cells.

Authors: Jaideep S Dudani; Daniel R Gossett; Henry T K Tse; Dino Di Carlo
Journal: Lab Chip Date: 2013-09-21 Impact factor: 6.799

8. Quantitative diagnosis of malignant pleural effusions by single-cell mechanophenotyping.

Authors: Henry T K Tse; Daniel R Gossett; Yo Sup Moon; Mahdokht Masaeli; Marie Sohsman; Yong Ying; Kimberly Mislick; Ryan P Adams; Jianyu Rao; Dino Di Carlo
Journal: Sci Transl Med Date: 2013-11-20 Impact factor: 17.956

9. Protective effect of methylcellulose and other polymers on insect cells subjected to laminar shear stress.

Authors: S Goldblum; Y K Bae; W F Hink; J Chalmers
Journal: Biotechnol Prog Date: 1990 Sep-Oct

Review 10. Mechanics, malignancy, and metastasis: the force journey of a tumor cell.

Authors: Sanjay Kumar; Valerie M Weaver
Journal: Cancer Metastasis Rev Date: 2009-06 Impact factor: 9.264

163 in total

1. Cell Mechanics: Combining Speed with Precision.

Authors: Hans M Wyss
Journal: Biophys J Date: 2015-11-17 Impact factor: 4.033

2. Microconstriction arrays for high-throughput quantitative measurements of cell mechanical properties.

Authors: Janina R Lange; Julian Steinwachs; Thorsten Kolb; Lena A Lautscham; Irina Harder; Graeme Whyte; Ben Fabry
Journal: Biophys J Date: 2015-07-07 Impact factor: 4.033

3. High-throughput and label-free parasitemia quantification and stage differentiation for malaria-infected red blood cells.

Authors: Xiaonan Yang; Zhuofa Chen; Jun Miao; Liwang Cui; Weihua Guan
Journal: Biosens Bioelectron Date: 2017-07-08 Impact factor: 10.618

Real-time deformability cytometry: on-the-fly cell mechanical phenotyping.

Review 1. Mitotic spindle assembly and chromosome segregation: refocusing on microtubule dynamics.

2. Sorting cells by size, shape and deformability.

Review 3. The actin cytoskeleton in spindle assembly and positioning.

4. Viscoelasticity as a biomarker for high-throughput flow cytometry.

5. The regulatory role of cell mechanics for migration of differentiating myeloid cells.

6. Deformation of red blood cells in capillaries.

7. Pinched-flow hydrodynamic stretching of single-cells.

8. Quantitative diagnosis of malignant pleural effusions by single-cell mechanophenotyping.

9. Protective effect of methylcellulose and other polymers on insect cells subjected to laminar shear stress.

Review 10. Mechanics, malignancy, and metastasis: the force journey of a tumor cell.

1. Cell Mechanics: Combining Speed with Precision.

2. Microconstriction arrays for high-throughput quantitative measurements of cell mechanical properties.

3. High-throughput and label-free parasitemia quantification and stage differentiation for malaria-infected red blood cells.

4. Myosin II Activity Softens Cells in Suspension.

5. FACS-style detection for real-time cell viscoelastic cytometry.

6. High-Throughput Microfluidic Characterization of Erythrocyte Shapes and Mechanical Variability.

7. Lab-on-a-chip based mechanical actuators and sensors for single-cell and organoid culture studies.

8. Forces, Flows, Fluorescence, and μFluidics.

9. Unbiased High-Precision Cell Mechanical Measurements with Microconstrictions.

10. Time-stretch microscopy on a DVD for high-throughput imaging cell-based assay.