| Literature DB >> 2564112 |
F Spielberg1, C M Kabeya, R W Ryder, N K Kifuani, J Harris, T R Bender, W L Heyward, T C Quinn.
Abstract
Five rapid, visually read assays for detection of antibody against human immunodeficiency virus (HIV) were evaluated on fresh serum samples from 4000 prospective blood donors at Mama Yemo Hospital, Kinshasa, Zaïre. The sensitivity of the assays, based on 214 specimens positive by western blot, ranged from 84.6% to 99.1%. The specificity, based on 3664 samples negative by enzyme-linked immunosorbent assay (ELISA) or western blot, ranged from 92.7% to 98.8%. Three readers scored each test result independently; disagreement about test interpretation occurred in 1.2-8.3% of the specimens. There was no correlation between assay performance and assay principle (agglutination or dot immunobinding) or antigen source (viral lysate or recombinant). Assays such as these can be readily implemented in a developing country transfusion centre, where blood screening by ELISA is not practicable.Entities:
Keywords: Acquired Immunodeficiency Syndrome; Africa; Africa South Of The Sahara; Americas; Developed Countries; Developing Countries; Diseases; Economic Factors; Error Sources; Evaluation; Evaluation Methodology; Examinations And Diagnoses; False Negative Reactions; False Positive Reactions; French Speaking Africa; Government Agencies; Hiv Infections; Laboratory Examinations And Diagnoses; Laboratory Procedures; Measurement; Middle Africa; North America; Northern America; Organizations; Reliability; Research Methodology; Screening; Technology; United States; Usaid; Viral Diseases; Zaire
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Year: 1989 PMID： 2564112 DOI： 10.1016/s0140-6736(89)91610-3
Source DB: PubMed Journal: Lancet ISSN： 0140-6736 Impact factor: 79.321