Enantioseparations of Chiral Ruthenium(II) Polypyridyl Complexes Using HPLC with Macrocyclic Glycopeptide Chiral Stationary Phases (CSPs).

A high performance liquid chromatographic method using macrocyclic glycopeptide chiral stationary phases (CSPs) was used to separate enantiomers of seven ruthenium(II) polypyridyl complexes. Among the five different CSPs, the Chirobiotic T2 was most effective and baseline separated all complexes. All complexes show the same elution order with the Δ-enantiomer being retained longer than the Λ-enantiomer. The mobile phase composition, including organic modifier type, organic modifier percent, salt type, and salt concentration, produced significant effects on the enantioresolution.