Prevalence of Helicobacter pylori in Patients With Dyspepsia.

BACKGROUND: The prevalence rate of gastric infections caused by Helicobacter pylori is different in between as well as within various countries, including Iran.
OBJECTIVES: This study was aimed to evaluate the prevalence of H. pylori in Iranian adult patients with dyspepsia. PATIENTS AND METHODS: A total of 548 patients with dyspepsia referred to Namazi Hospital, a referral center for gastrointestinal diseases in Shiraz, south of Iran, were evaluated in a cross-sectional study. The diagnosis of dyspepsia was based on clinical findings. Upper gastrointestinal endoscopy was performed for all the patients and two antral biopsies were taken from all the included ones. The patients were categorized based on the endoscopic findings, to four groups: normal,abnormal nonulcerative,ulcerative, andcombination of 2 and 3.Two positive test results (Gram staining, rapid urease test, and culture) was considered as a positive-definite H. pylori infection for each patient.
RESULTS: From the 548 included patients (238 males and 310 females), H. pylori was detected in 170 (31.2%). The mean age was 40.38 ± 15.39 years old. H. pylori infection was detected in 26.1% of male and 34.8% of female patients and its prevalence increased with age. Eighty three (48.8%) patients were positive for H. pylori infection, of which, 12.4% had normal and 36.4% had abnormal nonulcerative endoscopic findings; 17.1% of patients were ulcerative and 34.1% had ulcer with or without concurrent abnormality.
CONCLUSIONS: Findings from this study showed a lower prevalence of H. pylori infection than other studies and its prevalence increased with age. There was no association between sex and infection. The most common endoscopic abnormality in H. pylori-positive patients was ulcerative lesion.

1. Background

Helicobacter pylori is a Gram-negative bacteria with strong association with upper gastrointestinal diseases such as peptic ulcer diseases (gastric ulcer, duodenal ulcer) and malignancies (gastric cancer, lymphoma), reported worldwide and in Iran (1-3). In developing countries, H. pylori is typically acquired in early childhood and may persist for a lifetime if left untreated (4, 5). In adults, the H. pylori infection rate increases with age (6, 7). Oral-oral and fecal-oral routes are the most common modes of H. pylori transmission in developing countries. Less common modes of transmission are zoonotic and iatrogenic (such as endoscopic or through dental procedures) transmissions (8, 9).

In several previous studies, there were differences in the prevalence rate of H. pylori infection between countries (1, 10-14). It can be due to diverse contributing factors including socioeconomic status, geographical or living conditions, and ethnicity or location of each population (1). Dyspepsia is a medical condition characterized by chronic or recurrent pain in the upper abdomen, which is considered as one of the most common causes of patients’ referrals to gastroenterology centers (15).

The definition of dyspepsia has been elusive with variety of proposed descriptions (16). Dyspepsia is defined based on the recent Asian Consensus Report as a symptom or set of symptoms that is (are) considered to originate from the gastroduodenal region. In this consensus report, the dyspepsia symptoms are epigastric pain, epigastric burning, postprandial fullness, early satiation, and others, including bloating in the upper abdomen, nausea, vomiting and belching. Chronic dyspeptic symptoms can be continuous, intermittent, or recurrent. Most of the consensus members agreed that symptom duration of equal or more than three months might be enough for diagnosis of dyspepsia. Functional dyspepsia is defined as chronic dyspeptic symptoms in the absence of organic, systemic, or metabolic condition (s), which is (are) likely to explain the symptoms (16). In the western world, the reported prevalence of dyspepsia ranged 25-50% (17, 18). In the south-western United States, dyspepsia and H. pylori-related gastrointestinal diagnoses were remarkably common, with an annual incidence of 13 per 1000 individuals (15).

Alazmi et al. (12) demonstrated that the overall prevalence of patients with H. pylori infection and dyspepsia was 49.7% in Kuwait. In a systematic review by Amini et al. (19), the prevalence of dyspepsia in Iran varied between 2.2% to 29.9 %. Understanding the latest prevalence of H. pylori infection in patients with dyspepsia and determining its associations with endoscopic findings and pathologic changes in gastric mucosa can be useful for health-related purposes in every region and country. Some reports from Asia and Iran have demonstrated that the prevalence rate of H. pylori infection has declined rapidly in these regions (10, 11).

2. Objectives

This study was conducted to determine the prevalence rate of H. pylori infection among a population of adult patients with dyspepsia in Shiraz, south of Iran, using Gram staining, rapid urease test, and culture. We also hoped to find the rate of H. pylori infection in different endoscopic findings.

3. Patients and Methods

3.1. Study Population

After obtaining the approval of the university ethical committee (91-01-36-4703) as well as written informed consent from the patients, all the patients with dyspepsia attending Namazi Hospital in Shiraz; as a major referral center for gastrointestinal diseases in south of Iran, were recruited into the study between May and November 2012. The exclusion criteria for all the patients were age less than 18, history of H. pylori eradication, history of consumption of proton pump inhibitors (PPIs), H2-receptor antagonists, antacids or antibiotics within four weeks prior to the endoscopic evaluation, and history of gastric or esophageal surgery.

Diagnosis of dyspepsia was based on clinical findings. We defined dyspepsia if the patient had one or more of these symptoms with duration of three months or longer: postprandial fullness, early satiation, epigastric pain, epigastric burning, bloating in the upper abdomen, nausea, vomiting, and belching (16). Upper gastrointestinal endoscopy was performed for all the patients with dyspepsia as an objective assessment for the diagnosis of causes was also performed. We categorized our patients based on the endoscopic finding into four groups:

1- normal, 2- abnormal nonulcerative (any evidence of mucosal lesion without ulcer, e.g. erosion, erythema, nodularity, atrophy, white plaque, and petechiae), 3- ulcerative, and 4-combination of 2 and 3. Two antral biopsies were performed for all the included patients with normal and abnormal endoscopy results. Biopsy was also performed for any abnormal lesion. After the endoscopy, the biopsy samples were transferred to the lab under an appropriate condition for pathology evaluation. All the samples were evaluated by rapid urease test and Gram staining for the presence of H. pylori. If one of these test results was negative, the biopsy samples were gently cultured using standard methods (11). We defined H. pylori as positive if two of these test results (Gram staining, rapid urease test, and culture) were positive.

3.2. Statistical Analysis

All the data were expressed as mean ± standard error. Chi square test was used and significance was defined as a P value < 0.05. Data analysis was performed with SPSS software (version 19, SPSS Inc., Chicago, Illinois, USA).

4. Results

The endoscopic findings and the H. pylori test results of 631 patients with dyspepsia were evaluated retrospectively in this cross-sectional study. A total of 548 patients with a mean age of 40.38 ± 15.39 years old were enrolled in the study and 83 patients were excluded. Of the included patients, 238 (43.4%) were male and 310 (56.6%) were female. There was no association between sex and infection (P = 0.57). Of the 548 patients tested, 170 (31%) were positive and 378 (69%) were negative for H. pylori infection. H. pylori infection was observed in 26.1% of male and 34.8% of female patients (Table 1) and its prevalence increased with age. The overall prevalence of H. pylori infection had ha sharp increase from the middle-aged patients (5.3% in 30-34-year-old and 5.8% in 35-44-year-old patients) to 19.8% in those aged 45 or older (Table 1). As the endoscopy results indicated, 83 (48.8%) H. pylori-positive patients had normal or abnormal nonulcerative endoscopic findings (groups 1 and 2) and 87 (51.2%) had ulcer with or without concurrent abnormality (groups 3 and 4).

Table 1.

Prevalence and Patterns of Endoscopic Findings in H. pylori Infection

Variable	Patients (%)	H. pylori-Positive, No. (%)
Gender
Male	238 (43.4)	62 (26.1)
Female	310 (56.6)	108 (34.8)
Total	548 (100)	170 (31)
Age
< 35	211 (38.5)	29 (5.3)
35-44	145 (26.4)	32 (5.8)
> 45	192 (35.1)	109 (19.8)

5. Discussion

H. pylori infection is a major cause of gastroduodenal diseases; however, there have been different reports about the prevalence rate of this infection between countries (1, 10, 11). Several studies from Asia and Middle East including China, South Korea, Iran, and Kuwait have shown that the prevalence of H. pylori has declined in these areas in the recent years (10-13). In the present study, H. pylori positivity was 31% (Table 1), which was surprisingly lower than other local reports (2, 3, 11). In previous reports from Shiraz it was shown that more than 85% of Iranian adults carried IgG against H. pylori (2). In addition, histological evidence of H. pylori infection was recorded in 89.2% of biopsies in another report from north of Iran (3). Recently, in a survey conducted by Farshad et al. (11) in Iran, the prevalence of H. pylori in a total of 522 patients who underwent endoscopic evaluation was 47.9%.

Interestingly, the results of this study showed a lower prevalence of H. pylori in comparison with other reports from Asia and Middle East. Goh (14) reported that the overall prevalence rate of H. pylori in a Malaysian population with dyspepsia was 49.0%. In this study, two antral biopsies were performed for diagnosis of H. pylori, using a rapid urease test. In Alazmi et al. (12) research in Kuwait, an overall prevalence rate of 49.7% was reported in 362 patients with dyspepsia. In another report from India, Poddar and Yachha (6) demonstrated that almost 80% of the population in India was infected with H. pylori. Ahmed et al. (20) also showed that the overall prevalence of H. pylori in India was 80%. In this study, the diagnosis of H. pylori was established by polymerase chain reaction (PCR) amplification of the 16S rRNA gene of H. pylori. As expected, similar to several other studies (10-13) our research showed that the prevalence of H. pylori was declined.

According to the present study, the most common endoscopic abnormality in H. pylori-positive patients was ulcerative lesion, comprising groups 3 and 4 (51.2%) (Table 2). H. pylori infection can be responsible for gastroduodenal ulcer. This association has been shown in previous studies (14, 21). Goh (14) reported that H. pylori prevalence differed based on endoscopic findings. Similar results were reported in 1990 by Soll (21). Regarding the sex, this study showed that H. pylori positivity had predominance in females; however, no significant association between sex and infection rate was shown. A vast variety of results have been reported regarding the predominance of either males or females; but, it is generally concluded that there is no significant association between the infection rate of H. pylori and sex (1, 12, 20). There was a significant association between the H. pylori infection prevalence and age in our study, which was similar to some other researches (1, 12, 20). The findings of this cross-sectional study on dyspepsia showed a lower prevalence of H. pylori infection compared to other studies. Abnormal nonulcerative and ulcerative changes constituted the dominant findings in H. pylori-positive patients. It seems that this lower prevalence of H. pylori may be due to an improvement in the socioeconomic status of the Iranian population.

Table 2.

Pattern of Endoscopic Findings in H. pylori Infection

Variables	Patients, No. (%)
Endoscopic findings
Group 1 (normal)	21 (12.4)
Group 2 (abnormal nonulcerative)	62 (36.4)
Group 3 (ulcerative)	29 (17.1)
Group 4 (combination of groups 2 and 3)	58 (34.1)