pH level as a marker for predicting death among patients with Vibrio vulnificus infection, South Korea, 2000-2011.

Vibrio vulnificus infection can progress to necrotizing fasciitis and death. To improve the likelihood of patient survival, an early prognosis of patient outcome is clinically important for emergency/trauma department doctors. To identify an accurate and simple predictor for death among V. vulnificus-infected persons, we reviewed clinical data for 34 patients at a hospital in South Korea during 2000-2011; of the patients, 16 (47%) died and 18 (53%) survived. For nonsurvivors, median time from hospital admission to death was 15 h (range 4-70). For predicting death, the areas under the receiver operating characteristic curves of the Acute Physiology and Chronic Health Evaluation (APACHE) II score and initial pH were 0.746 and 0.972, respectively (p = 0.005). An optimal cutoff pH of <7.35 had a sensitivity of 100% and specificity of 83%. Compared with the APACHE II score, the initial arterial blood pH level in V. vulnificus-infected patients was a more accurate predictive marker for death.

Vibrio vulnificus is a motile, halophilic, rod-shaped gram-negative pathogen that lives in estuarine environments (). During the last decade, the prevalence of V. vulnificus infection has increased worldwide (–). In the United States, the annual number of V. vulnificus infections reported to the 10-state Foodborne Disease Active Surveillance Network (http://www.cdc.gov/foodnet/) increased from 0.01 cases/100,000 persons in 1996 to 0.05 cases/100,000 persons in 2010 ().

V. vulnificus infections, which are mainly characterized by skin and soft-tissue infections or septicemia, can develop a fulminant course (,). The severe form of V. vulnificus soft-tissue infection, necrotizing fasciitis, often has adverse outcomes, including death. In such cases, death can occur within 48 h of hospital admission, especially if the infection is associated with the development of sepsis or septic shock, which increases the case-fatality rate to 26%–71% (–). For cases of V. vulnificus infection caused by the consumption of contaminated shellfish, the death rate is ≈53%, and the rate is higher (67%) among patients with liver disease (). The death rate may increase to 100% in patients with septicemia if treatment is delayed for 72 h after symptom onset ().

Surgery, including fasciotomy, debridement, and limb amputation, early in V. vulnificus infection has been advocated by some as a means for improving patient outcomes (,,). However, the proper timing for surgery is debatable: in the early stages of infection, patients may not be stable enough to be moved to the operating room, and patients may have little chance of surviving if surgery is delayed until a later stage of infection (). Because V. vulnificus infections can be life threatening, it is critical for emergency/trauma department doctors, including surgeons who make decisions regarding surgery for necrotizing fasciitis, to have early knowledge of a patient’s prognosis.

Some clinical data are available regarding the prognostic factors for patients with fatal V. vulnificus infection (,–). Higher Acute Physiology and Chronic Health Evaluation (APACHE) II and Mortality in Emergency Department Sepsis (MEDS) scores have been reported to be significant prognostic indicators for V. vulnificus–infected patients (,). However, the APACHE II and MEDS scoring systems might be too complex to use quickly in an emergency setting. Thus, to find a marker to predict the risk for death among V. vulnificus–infected patients, we investigated the initial clinical and laboratory data available in hospital emergency departments. The aims of our study were to describe the clinical outcomes of V. vulnificus–infected patients and identify an accurate and simple predictive marker for death.

Materials and Methods

Patients

For the study, we identified 34 V. vulnificus–infected patients (>18 years of age) who had been admitted during January 2000–December 2011 to Chosun University Hospital, a tertiary teaching hospital in Gwangju, South Korea. V. vulnificus infection was diagnosed on the basis of blood and/or wound culture results. Clinical isolates were identified by using a Vitek II automated system (bioMérieux, Marcy l’Étoile, France). The study was approved by the Ethics in Human Research Committee of Chosun University Hospital.

Data Collection and Definitions

Demographic information and data regarding patients’ clinical manifestations, laboratory variables, operations, antimicrobial drug regimens, underlying diseases, and time to death were obtained by chart review. In Chosun University Hospital, arterial blood gas analysis and blood cultures are routinely performed before starting empirical antimicrobial drug treatment in patients with signs or symptoms of infection.

The use of an appropriate antimicrobial drug regimen was noted if a patient had been administered ceftriaxone, cefotaxime, or ciprofloxacin alone or in combination with doxycycline. Chronic liver disease was noted if a patient had shown clinical or laboratory signs of chronic liver disease caused by alcoholism or by hepatitis B or C virus. Fasciotomy was noted if surgery had been performed for necrotizing fasciitis. Death was defined as dying while in the hospital.

Statistical Analyses

We compared demographic and clinical characteristics for patients who survived and those who did not by using t-tests for continuous variables and χ2 tests for categorical variables. For APACHE II scores and pH levels at admission, we calculated the areas under the receiver operating characteristic curves (AUROCs) for predicting death caused by V. vulnificus infection. Sensitivities, specificities, positive predictive values, negative predictive values, positive likelihood ratios (how much to increase the probability of survival if the test is positive), and negative likelihood ratios (how much to decrease the probability of survival if the test is negative) of various APACHE II scores and pH cutoff levels on admission were calculated (). The Youden index was used to determine the optimal cutoff value ().

Multivariate analyses were performed by using the Cox proportional hazards model; variables that showed a p value of <0.05 in univariate analyses were included in these analyses. Of the variables for arterial blood gas analysis, pH was the only variable included in the model, and the APACHE II score was excluded from the multivariate model because of collinearity with pH and creatinine. Survival curves were constructed by using the Kaplan–Meier method, and a log-rank test was used for comparison. Survival was measured from the time of hospital admission to the time of death. We evaluated death status as death occurring <120 h after admission; patients who lived >120 h after admission were not included in the death statistics. A 2-tailed p value of <0.05 was considered to indicate statistical significance. All statistical analyses were performed by using SAS software, version 8.2 (SAS Institute Inc., Cary, NC, USA).

Results

Clinical Outcomes for Patients with V. vulnificus Infection

Of the 34 patients with a diagnosis of V. vulnificus infection, 3 (9%) were female and 31 (91%) were male. A total of 25 patients had a history of consuming raw seafood, 5 had a wound exposure to seawater, and 4 were unaware of their exposure source. Of the 32 patients who had chronic liver disease, 27 (84%) had chronic alcoholism and 5 (16%) had hepatitis B virus infection.Of the 34 total patients, 16 (47%) died and 18 (53%) survived. The median time between arrival at the hospital and death was 15 h (range 4–70 h). For patients who survived and those who did not, the differences in underlying diseases, interval between symptom onset and hospital admission, initial appropriate antimicrobial drug treatment, and surgical treatment were not significant (Table 1).

Table 1

Demographic and clinical features for 34 patients in a study looking for predictors of death among persons with Vibrio vulnificus infection, South Korea, 2000–2011*

Variable	Nonsurvivors, n = 16†	Survivors, n = 18†	p value
Sex			0.346
M	15 (93.8)	15 (83.3)
F	1 (6.3)	3 (16.7)
Age, y	58 (43–74)	57 (45–67)	0.740
Interval, d, between symptom onset and hospital admission	3 (1–5)	3 (0–7)	0.304
Route of exposure
Consumption of seafood	11 (69)	14 (78)	0.565
Wound exposure to seawater	3 (19)	2 (11)	0.723
Unknown	2 (12)	2 (22)	0.632
Underlying condition
Chronic liver disease	15 (94)	17 (94)	0.872
Chronic alcoholism	13 (81)	14 (78)	0.803
Hepatitis B virus infection	2 (13)	3 (17)	0.732
Hepatitis C virus infection	0	0	NA
Signs and symptoms at hospital admission
Any gastrointestinal symptom	7 (54.8)	5 (27.8)	0.331
Any skin and soft tissue lesion	12 (75.0)	15 (83.3)	0.549
Bacteremia present	10 (63)	8 (44)	0.292
Surgery performed	7 (44)	8 (44)	0.967
Appropriate antimicrobial drug treatment received	13 (81)	14 (78)	0.810

*NA, not applicable.
†Except for age, data are numbers (%) or medians (range).

Predictive Markers for Death

Univariate analysis showed that activated partial thromboplastin time and levels of pH, partial pressure of oxygen, serum bicarbonate, blood urea nitrogen, creatinine, aspartate transaminase, and alanine transaminase were significantly different between patients who survived and those who did not survive (Table 2). In multivariate analysis, initial pH on arterial blood gas analysis and serum creatinine level were significant risk factors for death (Table 3).

Table 2

Laboratory results 34 patients in a study looking for predictors of death among persons with Vibrio vulnificus infection, South Korea, 2000–2011*

Variable	Reference values	Values for patients		p value
		Nonsurvivors, n = 16	Survivors, n = 18
Hematologic values
Leukocyte count/μL	4,000–8,000	6,721 (1,030–25,950)	9,687 (1,940–21,770)	0.167
Hemoglobin, g/dL	12.0–16.0	12.5 (7.3–17.8)	11.5 (8.2–15.5)	0.248
Platelets, 103/μL	150–400	62 (13–147)	81 (13–243)	0.330
PT, s	9.4–12.5	17.7 (10.5–30.4)	14.9 (10.2–19.5)	0.111
aPTT, s	28.0–44.0	48.5 (28.8–103.0)	37.4 (21.5–57.5)	0.040
Arterial blood gas analysis values
pH	7.350–7.450	7.08 (6.82–7.36)	7.41 (7.22–7.50)	<0.001
HCO3, mmol/L	21.0–28.0	8.4 (3.6–13.9)	18.6 (8.7–25.6)	<0.001
PO2, mm Hg	83.0–108.0	103.4 (69.2–157.0)	86.6 (52.3–162.8)	0.035
PCO2, mm Hg	35.0–45.0	26.7 (14.3–41.0)	28.8 (21.6–38.0)	0.314
Clinical chemistry values
Albumin, g/dL	3.50–5.20	2.63 (2.07–3.00)	2.82 (1.66–3.84)	0.201
BUN, mg/dL	8.0–20.0	31.8 (10.0–56.9)	22.6 (9.6–49.7)	0.043
Creatinine, mg/dL	0.5–1.3	3.59 (1.52–5.90)	1.76 (0.60–3.90)	<0.001
Glucose, mg/dL	60–109	134 (22–306)	141 (40–300)	0.764
Bilirubin, mg/dL	0.2–1.2	7.5 (0.5–48.3)	2.3 (0.5–4.1)	0.094
Liver enzyme values
AST, IU/L	5–40	443 (43–1,696)	111 (30–323)	0.006
ALT, IU/L	5–40	100 (22–290)	57 (17–133)	0.020
APACHE II score		18 (10–31)	14 (10–32)	0.036

*Data are expressed as median (range). ALT, alanine aminotransferase; APACHE, Acute Physiology and Chronic Health Evaluation; aPTT, activated partial thromboplastin time; AST, aspartate aminotransferase; BUN, blood urea nitrogen; HCO3, serum bicarbonate; PCO2, carbon dioxide partial pressure; PO2, partial pressure of oxygen; PT, prothrombin time.

Table 3

Prognostic factors for death, as determined by multivariate analysis, among patients with Vibrio vulnificus infection, South Korea, 2000–2011*

Variable	Relative risk (95% CI)	p value
pH (per 0.1 increase)	0.441 (0.305–0.637)	<0.001
Creatinine (per mg/dL increase)	2.114 (1.105–4.043)	0.023
BUN (per mg/dL increase)	1.046 (0.981–1.115)	0.171
AST (per IU/L increase)	0.998 (0.995–1.001)	0.221
ALT (per IU/L increase)	1.014 (0.991–1.039)	0.236
aPTT (/s increase)	0.983 (0.935–1.033)	0.496

*ALT, alanine aminotransferase; aPTT, activated partial thromboplastin time; AST, aspartate aminotransferase; BUN, blood urea nitrogen.

We found a significant difference in survival for patients with different arterial pH levels at hospital admission (Figure 1). No patients with a pH level of <7.2 at admission survived; all patients with a pH level of >7.35 survived (Figure 1, 2). An optimal pH cutoff level of <7.35 had a sensitivity of 100%, specificity of 83%, positive predictive value of 84%, negative predictive value of 100%, positive likelihood ratio of 6.0, and negative likelihood ratio of 0. An optimal APACHE II cutoff score of >14 had a sensitivity of 75%, specificity of 67%, positive predictive value of 67%, negative predictive value of 75%, positive likelihood ratio of 2.3, and negative likelihood ratio of 0.3 (Table 4). The AUROC of pH for prediction of death was 0.972 (95% CI 0.924–1.000), and the AUROC of the APACHE II score was 0.746 (95% CI 0.595–0.933) (p = 0.005) (Figure 3).

Figure 1

Survival curves of patients, by pH level at hospital admission, in a study investigating predictors of death among persons with Vibrio vulnificus infection, South Korea, 2000–2011.

Figure 2

Numbers of surviving and nonsurviving patients, by pH level at hospital admission, in a study investigating predictors of death among persons with Vibrio vulnificus infection, South Korea, 2000–2011.

Table 4

Predictors of death among patients with Vibrio vulnificus infection, South Korea, 2000–2011*

Predictor	Sensitivity, %		Predictive value, %			Likelihood ratio
		Specificity, %	Positive	Negative		Positive	Negative
pH level
<7.30	87.5	94.4	93.3	89.5		15.63	0.13
<7.35	100.0	83.3	84.2	100.0		5.99	0
<7.40	100.0	61.1	69.6	100.0		2.57	0
APACHE II score
>13	87.5	61.1	66.7	84.6		2.25	0.20
>14	75.0	66.7	66.7	75.0		2.25	0.37
>15	75.0	77.8	75.0	77.8		3.38	0.32

*APACHE, Acute Physiology and Chronic Health Evaluation.

Figure 3

Receiver-operating characteristic curves (AUROCs) for pH level and Acute Physiology and Chronic Health Evaluation (APACHE) II score in a study investigating predictors of death among patients with Vibrio vulnificus infection, South Korea, 2000–2011. AUROC (95% CIs): pH level, 0.972 (range 0.924–1.000); APACHE II score,0.746 (range 0.595–0.933) (p = 0.005).

Discussion

In this study, approximately half of the patients with V. vulnificus infection died, and death occurred <72 h after hospital admission. Patient survival differed significantly by pH level at hospital admission, and the initial pH level for patients was a more accurate predictive marker for death than was the APACHE II score.

Many studies have asserted that antimicrobial drugs should be immediately administered to patients with suspected V. vulnificus infection (,,,). Other studies suggest that a combination of antimicrobial drugs (i.e., third-generation cephalosporin, ciprofloxacin, and doxycycline) is the best treatment for V. vulnificus infection (,). In addition, surgical intervention is necessary to remove necrotic tissue and bacteria (). Survival of patients with necrotizing fasciitis has been shown to improve when adequate debridement and fasciotomy are performed early in infection (,,,).

However, in our study there was no difference in the time from symptom onset to hospital admission and the timing of the initiation of appropriate antimicrobial drug treatment and surgical treatment for patients who survived and those who did not. This lack of difference may partly be due to the fact that patients died too early after admission to benefit from antimicrobial drug treatment or surgery. The median time to death for nonsurviving patients in our study was 15 h, even though they arrived at the hospital relatively early after symptom onset. The time to death in our study seems to be shorter than that in another study, which showed 11 (73%) of the 15 deaths occurred <72 h after arrival at the hospital ().

That earlier study and another study reported that APACHE II and MEDS scores at hospital admission were useful prognostic indicators in primary septicemia or wound infections caused by V. vulnificus (,). In another study, the level of V. vulnificus DNA was substantially higher in nonsurvivors than in survivors of V. vulnificus septicemia, and the V. vulnificus DNA load correlated with the APACHE II score (). Our data also showed that the APACHE II score may be useful for prediction of death in V. vulnificus–infected patients, although the AUROC for the APACHE II score was significantly less than that for initial pH.

In previous reports, hemorrhagic bullae formation, necrotizing fasciitis, and septic shock were risk factors for death in patients with V. vulnificus infection (,–). Although in our study, no skin or soft tissue lesions were associated with death, the pH and creatinine levels at hospital admission were independently associated with death of V. vulnificus–infected patients. Low arterial pH level might result from lactic acidosis caused by marked tissue hypoperfusion in shock and damage to extremities by necrotizing fasciitis (,). The finding that high creatinine level was associated with death also supports the possibility that hypoperfusion or sepsis contributed to the death of patients in this study because azotemia might result from renal hypoperfusion or septic renal injury.

Our study did have some limitations because of its retrospective design and the relatively small number of study patients, which may hamper generalization of the results. In addition, because most patients in this study had chronic liver disease, the study findings might not be applicable to patients with various concurrent diseases other than chronic liver disease.

Despite these limitations, our study may have relevant clinical implications. Our data demonstrate that the pH level determined by arterial blood gas analysis at hospital admission is more accurate than the APACHE II score as a marker for predicting death in V. vulnificus–infected patients. Arterial blood gas levels are easy to determine, and results are quickly available; thus, this test is useful in emergency or trauma departments.

We propose that arterial pH level should be determined for patients with suspected V. vulnificus infection as soon as possible after hospital admission. When the arterial pH level is low (<7.2), patient outcome may be unsatisfactory, regardless of emergency surgery and appropriate antimicrobial drug therapy. When the pH level is not low, patient outcome may be excellent if the patient receives appropriate antimicrobial drug treatment and, if needed, surgery.