Pra Oliveira1, Abm Fonseca2, E M Silva3, Tcl Coutinho1, M A Tostes1. 1. Department of Pediatric Dentistry, School of Dentistry, Fluminense Federal University, Rio de Janeiro, Brazil. 2. Department of Statistics, Fluminense Federal University, Rio de Janeiro, Brazil. 3. Analytical Laboratory of Restorative Biomaterials, School of Dentistry, Federal Fluminense University, Rio de Janeiro, Brazil.
Abstract
BACKGROUND: This study evaluated the effect of pastes containing casein phosphopeptide-amorphous calcium phosphate (CPP-ACP) with and without fluoride on enamel demineralization. METHODS: Human enamel blocks were used and after incipient caries-like lesions were formed, they were assigned to four groups: G1 - saliva; G2 - MI Paste (RecaldentTM ); G3 - MI Paste Plus (RecaldentTM 900 ppm as NaF); and G4 - Crest™ (1.100 ppm as NaF). The specimens were soaked in demineralizing solution for 6 hours and remineralized in artificial saliva for 18 hours alternatively for 10 days. The dentifrice was prepared with deionized water in a 1:3 proportion (w/w) or applied undiluted in the case of the CPP-ACP formula. Demineralized enamel changes were analysed by surface microhardness (SMH), 3D-profilometry and SEM. Data were analysed by non-parametric Kruskal-Wallis and Mann-Whitney test comparisons and one-way ANOVA and Tukey's HSD post hoc test (α = 0.05). RESULTS: The SMH values observed in the G2 (47.8 ± 28.5) and G3 (53.6 ± 27.6) groups were different from that of G4 (90.2 ± 17.1), which were significantly higher than that found in G1 (39.4 ± 14.2). The %SMH was significantly lower in G4 when compared to G1 (p < 0.001) and G3 (p < 0.05). CONCLUSIONS: MI Paste Plus demonstrated a greater protective effect against demineralization than MI Paste and showed smoother surfaces.
BACKGROUND: This study evaluated the effect of pastes containing casein phosphopeptide-amorphous calcium phosphate (CPP-ACP) with and without fluoride on enamel demineralization. METHODS:Human enamel blocks were used and after incipient caries-like lesions were formed, they were assigned to four groups: G1 - saliva; G2 - MI Paste (RecaldentTM ); G3 - MI Paste Plus (RecaldentTM 900 ppm as NaF); and G4 - Crest™ (1.100 ppm as NaF). The specimens were soaked in demineralizing solution for 6 hours and remineralized in artificial saliva for 18 hours alternatively for 10 days. The dentifrice was prepared with deionized water in a 1:3 proportion (w/w) or applied undiluted in the case of the CPP-ACP formula. Demineralized enamel changes were analysed by surface microhardness (SMH), 3D-profilometry and SEM. Data were analysed by non-parametric Kruskal-Wallis and Mann-Whitney test comparisons and one-way ANOVA and Tukey's HSD post hoc test (α = 0.05). RESULTS: The SMH values observed in the G2 (47.8 ± 28.5) and G3 (53.6 ± 27.6) groups were different from that of G4 (90.2 ± 17.1), which were significantly higher than that found in G1 (39.4 ± 14.2). The %SMH was significantly lower in G4 when compared to G1 (p < 0.001) and G3 (p < 0.05). CONCLUSIONS: MI Paste Plus demonstrated a greater protective effect against demineralization than MI Paste and showed smoother surfaces.