Alper Biler1, Sait Yucebilgin2, Fatih Sendag2, Levent Akman2, Ali Akdemir2, Utku Ates3, Yigit Uyanikgil3, Ozlem Yilmaz-Dilsiz3, Ebru Sezer4. 1. Fatsa Gynecology and Maternity Training and Research Hospital of TC Ministry of Health, Ordu, Turkey. 2. Department of Obstetrics and Gynecology, Faculty of Medicine, Ege University, Bornova, İzmir, Turkey. 3. Department of Histology and Embryology, Faculty of Medicine, Ege University, Bornova, İzmir, Turkey. 4. Department of Biochemistry, Faculty of Medicine, Ege University, Bornova, İzmir, Turkey.
Abstract
BACKGROUND: To determine the effects of different intraabdominal pressure (IAP) on the ovaries in a laparoscopic rat model. OBJECTIVES: The aim of the study was to determine the effects on the ovaries of different intraabdominal pressures (IAP) in laparoscopic surgery in a rat model. MATERIAL AND METHODS: Thirty-two post-pubertal nonpregnant Sprague-Dawley rats were divided randomly into four groups. In the control group, no intraabdominal pressure (IAP) was applied. In Group Pp10 and Group Pp15, an IAP of 10 and 15 mm Hg, respectively, were applied by carbon dioxide insufflation for 60 min, and a 30-min desufflation was carried out. In Group IPp15, a 15 mm Hg IAP was applied for 10 min, and then CO2 was desufflated for 10 min. After this ischemic preconditioning, IAP was established at 15 mm Hg for 60 min, after which CO2 was desufflated for 30 min. Erythrocyte and ovarian tissue malondialdehyde (MDA) and histopathologic damage scores were evaluated. RESULTS: In Groups Pp10 and Pp15, ovarian tissue MDA values were significantly increased compared to the control group. In Groups Pp10 and Pp15, erythrocyte MDA values were significantly increased when compared to Group IPp15 and the control group. Ovarian histopatological assesment scores were significantly higher in Group Pp15 than in Groups Pp10 and IPp15. CONCLUSIONS: Pneumoperitoneum causes injuries to abdominal organ such as the ovaries. The ischemic preconditioning method is more effective in reducing oxidative stress due to laparoscopic pneumoperitoneum than low-pressure pneumoperitoneum methods.
BACKGROUND: To determine the effects of different intraabdominal pressure (IAP) on the ovaries in a laparoscopic rat model. OBJECTIVES: The aim of the study was to determine the effects on the ovaries of different intraabdominal pressures (IAP) in laparoscopic surgery in a rat model. MATERIAL AND METHODS: Thirty-two post-pubertal nonpregnant Sprague-Dawley rats were divided randomly into four groups. In the control group, no intraabdominal pressure (IAP) was applied. In Group Pp10 and Group Pp15, an IAP of 10 and 15 mm Hg, respectively, were applied by carbon dioxide insufflation for 60 min, and a 30-min desufflation was carried out. In Group IPp15, a 15 mm Hg IAP was applied for 10 min, and then CO2 was desufflated for 10 min. After this ischemic preconditioning, IAP was established at 15 mm Hg for 60 min, after which CO2 was desufflated for 30 min. Erythrocyte and ovarian tissue malondialdehyde (MDA) and histopathologic damage scores were evaluated. RESULTS: In Groups Pp10 and Pp15, ovarian tissue MDA values were significantly increased compared to the control group. In Groups Pp10 and Pp15, erythrocyte MDA values were significantly increased when compared to Group IPp15 and the control group. Ovarian histopatological assesment scores were significantly higher in Group Pp15 than in Groups Pp10 and IPp15. CONCLUSIONS: Pneumoperitoneum causes injuries to abdominal organ such as the ovaries. The ischemic preconditioning method is more effective in reducing oxidative stress due to laparoscopic pneumoperitoneum than low-pressure pneumoperitoneum methods.