Nadia A Henriksen1, Joachim H Mortensen2, Lars T Sorensen3, Anne C Bay-Jensen2, Magnus S Ågren4, Lars N Jorgensen3, Morten A Karsdal2. 1. Digestive Disease Center, Bispebjerg Hospital, University of Copenhagen, Copenhagen, Denmark. Electronic address: nadiahenriksen@gmail.com. 2. Nordic Bioscience A/S, Herlev, Denmark. 3. Digestive Disease Center, Bispebjerg Hospital, University of Copenhagen, Copenhagen, Denmark. 4. Digestive Disease Center, Bispebjerg Hospital, University of Copenhagen, Copenhagen, Denmark; Copenhagen Wound Healing Center, Bispebjerg Hospital, University of Copenhagen, Copenhagen, Denmark.
Abstract
BACKGROUND: Disturbed metabolism in the extracellular matrix (ECM) contributes to formation of abdominal wall hernias. The aim of this study was to gain deeper insight into the ECM turnover in hernia patients by analyzing serum biomarkers specifically reflecting collagen synthesis and breakdown in the interstitial matrix (types I, III, and V collagens) and in the basement membrane (type IV collagen). MATERIAL AND METHODS: Patients with 3 different types of hernias were included: Primary unilateral inguinal hernia (n = 17), multiple hernias defined as ≥3 hernias (n = 21), and incisional hernia (n = 25). Patients without hernias scheduled to undergo elective operation for gallstones (n = 18) served as controls. Whole venous blood was collected preoperatively. Biomarkers for synthesis of interstitial matrix (PINP, Pro-C3, P5CP) and basement membrane (P4NP) as well as corresponding degradation (C1M, C3M, C5M, and C4M) were measured in serum by validated, solid-phase competitive assays. RESULTS: In inguinal hernia patients, the turnover of the interstitial matrix collagens type III (P < .042) and V (P < .001) was decreased compared with controls, whereas the turnover of the basement membrane collagen type IV was increased (P < .001). In incisional hernia patients, the turnover of type V collagen was decreased (P = .048) and the turnover of type IV collagen was increased compared with the hernia-free controls (P < .001). CONCLUSION: Hernia patients demonstrated systemically altered collagen metabolism. The serologic turnover profile of type IV collagens may predict the presence of inguinal and incisional hernia. Regulation of type IV collagen turnover may be crucial for hernia development.
BACKGROUND: Disturbed metabolism in the extracellular matrix (ECM) contributes to formation of abdominal wall hernias. The aim of this study was to gain deeper insight into the ECM turnover in herniapatients by analyzing serum biomarkers specifically reflecting collagen synthesis and breakdown in the interstitial matrix (types I, III, and V collagens) and in the basement membrane (type IV collagen). MATERIAL AND METHODS:Patients with 3 different types of hernias were included: Primary unilateral inguinal hernia (n = 17), multiple hernias defined as ≥3 hernias (n = 21), and incisional hernia (n = 25). Patients without hernias scheduled to undergo elective operation for gallstones (n = 18) served as controls. Whole venous blood was collected preoperatively. Biomarkers for synthesis of interstitial matrix (PINP, Pro-C3, P5CP) and basement membrane (P4NP) as well as corresponding degradation (C1M, C3M, C5M, and C4M) were measured in serum by validated, solid-phase competitive assays. RESULTS: In inguinal herniapatients, the turnover of the interstitial matrix collagens type III (P < .042) and V (P < .001) was decreased compared with controls, whereas the turnover of the basement membrane collagen type IV was increased (P < .001). In incisional herniapatients, the turnover of type V collagen was decreased (P = .048) and the turnover of type IV collagen was increased compared with the hernia-free controls (P < .001). CONCLUSION:Herniapatients demonstrated systemically altered collagen metabolism. The serologic turnover profile of type IV collagens may predict the presence of inguinal and incisional hernia. Regulation of type IV collagen turnover may be crucial for hernia development.
Authors: Jürgen Böhm; Frank Pianka; Nina Stüttgen; Junghyun Rho; Biljana Gigic; Yuzheng Zhang; Nina Habermann; Petra Schrotz-King; Clare Abbenhardt-Martin; Lin Zielske; Paul D Lampe; Alexis Ulrich; Markus K Diener; Cornelia M Ulrich Journal: Surgery Date: 2016-10-13 Impact factor: 3.982
Authors: J J Harlaar; H H Eker; K A Vakalopoulos; M C Cabezas; A C van der Ham; W W Vrijland; J Jeekel; J F Lange Journal: Hernia Date: 2017-04-12 Impact factor: 4.739