C Lebrun1, G Forzy2, N Collongues3, M Cohen4, J de Seze3, P Hautecoeur2. 1. Service de neurologie, hôpital Pasteur, CHU de Nice, 30, voie Romaine, 06002 Nice, France. Electronic address: LEBRUN.c@chu-nice.fr. 2. Centre catholique de Lomme, université catholique de Lille, 56, rue du Port, 59046 Lille, France. 3. Neurologie, CHU de Strasbourg, 1, place de L'hôpital, BP 426, 67091 Strasbourg cedex, France. 4. Service de neurologie, hôpital Pasteur, CHU de Nice, 30, voie Romaine, 06002 Nice, France.
Abstract
BACKGROUND: Although radiologically isolated syndrome (RIS) is a newly defined entity, incidental findings of T2 hypersignals on brain MRI can lead to misdiagnosis or useless investigations. The detection of oligoclonal bands (OCBs) in cerebrospinal fluid (CSF) is a major indicator that helps in diagnosis of subclinical inflammatory disease of the central nervous system, but lumbar puncture still remains an invasive option. METHODS: We have prospectively included patients with RIS, have compared the results of CSF and tear OCB detection by isoelectric focusing (IEF) and assessed concordance between OCB detection in tears and in CSF. Tears were collected using a Schirmer strip. RESULTS: In 45 recruited RIS patients, OCBs were detected in CSF for 55% (25/45) and in tears for 50% (21/42) of samples. CONCLUSIONS: We suggest that tear OCB detection may replace CSF OCB detection as a diagnostic tool in patients with RIS and be useful in follow-up.
BACKGROUND: Although radiologically isolated syndrome (RIS) is a newly defined entity, incidental findings of T2 hypersignals on brain MRI can lead to misdiagnosis or useless investigations. The detection of oligoclonal bands (OCBs) in cerebrospinal fluid (CSF) is a major indicator that helps in diagnosis of subclinical inflammatory disease of the central nervous system, but lumbar puncture still remains an invasive option. METHODS: We have prospectively included patients with RIS, have compared the results of CSF and tear OCB detection by isoelectric focusing (IEF) and assessed concordance between OCB detection in tears and in CSF. Tears were collected using a Schirmer strip. RESULTS: In 45 recruited RIS patients, OCBs were detected in CSF for 55% (25/45) and in tears for 50% (21/42) of samples. CONCLUSIONS: We suggest that tear OCB detection may replace CSF OCB detection as a diagnostic tool in patients with RIS and be useful in follow-up.
Authors: Farah Haddad; Samuel Boudet; Laurent Peyrodie; Nicolas Vandenbroucke; Patrick Hautecoeur; Gérard Forzy Journal: Med Biol Eng Comput Date: 2020-02-24 Impact factor: 2.602
Authors: Martin W Hümmert; Ulrich Wurster; Lena Bönig; Philipp Schwenkenbecher; Kurt-Wolfram Sühs; Sascha Alvermann; Stefan Gingele; Thomas Skripuletz; Martin Stangel Journal: Front Immunol Date: 2019-05-17 Impact factor: 7.561